Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 4 of 4
Full-Text Articles in Life Sciences
Engineering For Expression Of The Cold Regulated Barley Protein Hvcr21 In E. Coli, Kathleen Graupman
Engineering For Expression Of The Cold Regulated Barley Protein Hvcr21 In E. Coli, Kathleen Graupman
The Journal of Undergraduate Research
HVCR21 is a barley protein known to be cold regulated at the mRNA level. However, its function as well as the affect of low temperature on its translation are unknown. The purpose of this project was to engineer E. coli to express recombinant HVCR21. PGR primers were designed for the 5' and 3' ends of the HVCR21 coding region. The primers were also designed with a 5' SacI restriction site and a 3' Pst I restriction site. After ligation of the PCR product into the pCR4-TOPO vector, bacteria were transformed and plated and the successful transformant verified by PCR. The …
A Comparison Of Methods For On-Farm Determination Of Failure Of Passive Transfer Of Immunoglobulin To Dairy Calves, Ann Harvey
The Journal of Undergraduate Research
Two commercially available, qualitative, on-farm test kits (Midland BioProducts Inc., Boone, lA), utilizing either serum or whole blood to evaluate failure of passive transfer (FPT) of immunoglobulins, were evaluated using 38 Holstein calves. Results from the kits were compared with refractometry determination of serum proteins and radial immunodiffusion determinations specific for IgG (RID; Triple J Farms, Bellingham,WA). Blood samples were collected immediately following birth before first colostrum feeding and at 48 h. At birth, serum protein concentrations averaged 4.52 g/dl and IgG averaged 8.6 mg/dl, respectively, for refractometer and RID. Forty eight hours after feeding colostrum, serum protein concentrations averaged …
The Journal Of Undergraduate Research: Volume 01
The Journal Of Undergraduate Research: Volume 01
The Journal of Undergraduate Research
This is the complete issue of the South Dakota State University Journal of Undergraduate Research, Volume 1.
Gene Family Encoding Hordeum Vulgare Translation Elongation Factor 1 Alpha (Ef-1a): Assignment Based On Bioinformatic Analysis Of Cdna Sequences, Jackson Moeller
Gene Family Encoding Hordeum Vulgare Translation Elongation Factor 1 Alpha (Ef-1a): Assignment Based On Bioinformatic Analysis Of Cdna Sequences, Jackson Moeller
The Journal of Undergraduate Research
Search of international DNA database, Genbank, produced eight barley cDNA EFlα sequences with unique accession numbers. The length of these sequences varied from an incomplete 502 bp to a full length 1646 bp encoding a 447 amino acid protein. The knowledge that there are four EF-lα gene family members in barley led us to query whether all four genes were represented in the group of eight database submitted sequences. Also of great importance was the correct assignment of gene family member to its mRNA and protein expression profiles. Before we could begin to address the expression studies, we undertook the …