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Full-Text Articles in Life Sciences

Reverse Genetic Analysis Of Ehd1 For An Association Between Flowering Time And Seed Dormancy In Rice (Oryza Sativa L.), Yeter Karakoc Jan 2020

Reverse Genetic Analysis Of Ehd1 For An Association Between Flowering Time And Seed Dormancy In Rice (Oryza Sativa L.), Yeter Karakoc

Electronic Theses and Dissertations

This research aimed to understand the evolutionary mechanism of seed dormancy and flowering time and to provide basic knowledge for the manipulation of germination features in crop breeding. Flowering time and seed dormancy are two adaptive traits for flowering plants. Seed dormancy (SD) helps regulate the timing of germination to promote the survival of seed-bearing plants in adverse environments. Cereal crops and weedy/wild relatives, such as Asian cultivated rice and weedy rice (Oryza sativa L.), have weak and strong seed dormancy, respectively. Thus, weedy and cultivated rice has been used as a model system to investigate mechanisms of seed dormancy …


A Seed Dormancy Gene-Mediated Biotechnology To Mitigate Transgene Flow Into Weedy Rice, Luai Nahar Muhammad Jan 2019

A Seed Dormancy Gene-Mediated Biotechnology To Mitigate Transgene Flow Into Weedy Rice, Luai Nahar Muhammad

Electronic Theses and Dissertations

Transgene flows into wild/weedy relatives may cause ecological and economic problems. Seed dormancy is an adaptive trait that distributes germination over time and promotes persistence of weeds in agroecosystems. Silencing natural genes controlling seed dormancy (SD) could promote germination and reduce weed adaptability. The goal of this project was to develop a transgenic mitigation (TM) technology by linking to a primary transgene with an SD gene-silencing structure, as a tandem construct for transformation, to reduce the risk of gene flow into weed populations. In this research, the Bar (Bialaphos) gene for resistance to the glufosinate herbicide was used as a …


Map-Based Cloning And Molecular Characterization Of The Seed Dormancy 10 Locus In Rice (Oryza Sativa L.), Wirat Pipatpongpinyo Jan 2018

Map-Based Cloning And Molecular Characterization Of The Seed Dormancy 10 Locus In Rice (Oryza Sativa L.), Wirat Pipatpongpinyo

Electronic Theses and Dissertations

Seed dormancy is a key adaptive trait of both ecological and agricultural importance. Although many quantitative trait loci (QTL) have been associated with seed dormancy in cereal crops or model plants, a majority of them remain unknown for molecular identities and functions. Cereal crops and wild/weedy relatives usually have weak and strong seed dormancy, respectively, such as Asian cultivated rice vs. weedy rice (Oryza sativa L.). Previous research identified a set of seed dormancy QTL, including qSD10, in single plant-derived BC1F2 and BC1F3 populations from the backcross between the weedy rice line SS18-2 …


Genetic Component Effects Of Two Loci On Seed Dormancy, Awn, Low-Temperature Germination, Plant Height, And Flowering Time In Rice (Oryza Sativa L.), Ugur Korkmaz Jan 2018

Genetic Component Effects Of Two Loci On Seed Dormancy, Awn, Low-Temperature Germination, Plant Height, And Flowering Time In Rice (Oryza Sativa L.), Ugur Korkmaz

Electronic Theses and Dissertations

Seed dormancy (SD) is an adaptive trait of both ecological and agricultural importance. Cereal crops have been selected for reduced SD to promote germination, which also caused the pre-harvest sprouting (PHS) problem in crop production. The previous research identified a set of quantitative trait loci (QTL) for SD, including qSD1- 2 and qSD8, in the conspecific weedy and cultivated rice (Oryza sativa). qSD1-2 carries a dormancy-enhancing allele from the cultivated rice and has a pleiotropic effect on plant height. qSD8 carries a dormancy-enhancing allele from the weedy rice and tightly links to qAL8 for awn length. The …


Silencing Seed Dormancy Genes To Mitigate Risk Of Transgene Flow To Weedy Rice, Alexander Wireko Kena Jan 2017

Silencing Seed Dormancy Genes To Mitigate Risk Of Transgene Flow To Weedy Rice, Alexander Wireko Kena

Electronic Theses and Dissertations

The flow of fitness-enhancing transgenes from genetically modified crops into wild/weed relatives may cause serious ecological and economic consequences. Seed dormancy (SD) is a key adaptive trait that distributes germination over time, resulting in weed persistence in agroecosystems. Thus, silencing major genes controlling SD would reduce the adaptive fitness of weeds. SD-enhancing genes cloned from weedy rice include SD7-1, SD7-2, SD12a, SD12b, and SD12c. The goal of this study was to develop a transgenic mitigation (TM) strategy using SD gene-silencing structures as mitigating factors to reduce the risk of transgene flow to wild/weed populations. TM vector constructs consisted of the …