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Cytopathology Of Barley And Corn Leaf Tissue Infected With The Nd18 Strain Of Barley Stripe Mosaic Virus, Charles R. Mcmullen
Cytopathology Of Barley And Corn Leaf Tissue Infected With The Nd18 Strain Of Barley Stripe Mosaic Virus, Charles R. Mcmullen
Electronic Theses and Dissertations
The distribution of the ND18 strain of barley stripe mosaic virus (BSMV) in barley (Hordeum vulgare L.) and corn (Zea mays L.) leaf tissue was studied with light and electron microscopes. Virions were detected in the cytoplasm of barley mesophyll cells, subsidiary, guard and epidermal cells, vascular parenchyma cells, sieve and tracheary elements. Nuclear virus was found in barley mesophyll cells. Ultrastructural cytopathological changes were greatest in the inoculated leaf of barley which contained fewer virus particles than either primary acute or chronically infected tissues. Primary acute and chronically infected barley tissues contained intracellular virions scattered in the cytoplasm, in …
A Comparative Study Of Ribonuclease From Two Winter Barley Cultivars Based Upon Separation, Purification And Partial Characterization Of Rna-Degrading Enzymes, Raymond Sze-Chung Wong
A Comparative Study Of Ribonuclease From Two Winter Barley Cultivars Based Upon Separation, Purification And Partial Characterization Of Rna-Degrading Enzymes, Raymond Sze-Chung Wong
Electronic Theses and Dissertations
The differences in the response to KCl of the RNA-degrading enzymes of two barley cultivars, “Dicktoo” (a hardy type) and "Tennessee Winter” (a non-hardy type), were investigated. Two types of enzymes, ribonuclease (RNase) and nuclease, were separated from crude extracts by disc electrophoresis. The electrophoretic gels were assayed for RNA-degrading activity in the presence and absence of EDTA (EDTA is a nuclease inhibitor). By means of density gradient isoelectric focusing, the nuclease preparation from Tennessee Winter was shown to have an isoelectric point between 5.3 and 5.5, while the nuclease preparation from Dicktoo has three isoelectric points of 4.9, 5.5 …