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Analysis Of Starch Branching Enzyme 1 (Sbe1) Gene In Maize, Abiskar Gyawali
Analysis Of Starch Branching Enzyme 1 (Sbe1) Gene In Maize, Abiskar Gyawali
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A previous study revealed a quantitative trait locus (QTL) in maize on the short arm of chromosome 5 (5S) for endosperm amylose content. In that original study, both low and high amylose parental lines, H99ae and GEMS-0067 respectively, were homozygous recessive for amylose extender 1 (ae1) and the polymorphism responsible for this QTL was additive (semi-dominant). Located within the QTL interval is starch branching enzyme 1 (sbe1), which makes it a candidate gene. In order to test whether a polymorphism in sbe1 is the source of this QTL, we crossed a plant homozygous for ae1 and sbe1-Mu onto a …