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Evidence Of Plasmotomy In Blastocystis Hominis, Tan Tian Chye
Evidence Of Plasmotomy In Blastocystis Hominis, Tan Tian Chye
Tan Tian Chye
Blastocystis hominis has been regarded as an enigmatic parasite as many aspects of its basic biology remain uncertain. Many reproductive processes have been suggested for the organism; however, to date, only the binary fission has been proven. Plasmotomy is one of the modes of reproduction previously suggested to be seen in in vitro cultures. The present study provides trichrome and acridine orange staining evidence for the existence of nucleic acid suggestive of division of nucleus into multinucleate forms with the respective cytoplasm dividing giving rise to two or three progeny B. hominis. Transmission electron micrographs further confirmed that these daughter …
Amoeboid Form Of Blastocystis Hominis - A Detailed Ultrastructural Insight, Tan Tian Chye
Amoeboid Form Of Blastocystis Hominis - A Detailed Ultrastructural Insight, Tan Tian Chye
Tan Tian Chye
The amoeboid form of Blastocystis hominis has been reported infrequently, and its morphological descriptions have yielded conflicting and confusing reports. In the present study, we used the amoeboid forms seen predominantly in symptomatic patients infected with Blastocystis to provide detailed descriptions on the fine surface structure and intracellular morphology. Scanning electron microscopy revealed the irregular shape of the amoeboid form, with an intercalated fibrillar structure and a highly convoluted surface with deep indentations and projected pseudopodia. Transmission electron microscopy showed the existence of two types of amoeboid forms of B. hominis in in vitro culture, one with a large central …
Predominance Of Amoeboid Forms Of Blastocystis Hominis In Isolates From Symptomatic Patients, Tan Tian Chye
Predominance Of Amoeboid Forms Of Blastocystis Hominis In Isolates From Symptomatic Patients, Tan Tian Chye
Tan Tian Chye
Blastocystis hominis is one of the most common human parasites that inhabit the intestinal tract. Conflicting reports continue to exist regarding the existence and the functional role of the amoeboid forms in the life cycle of the parasite. The present study investigates the presence of these forms in 20 isolates obtained from ten symptomatic and asymptomatic patients respectively. A total of 10,000 parasite cells per ml from each isolate were inoculated into three culture tubes each containing 3 ml of Jones' medium supplemented with 10% horse serum, incubated at 37 degrees C. The contents were examined daily for 10 days. …