Open Access. Powered by Scholars. Published by Universities.®

Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

SelectedWorks

Selected Works

2002

Discipline
Keyword
Publication

Articles 1 - 27 of 27

Full-Text Articles in Life Sciences

Kryptoperidinium Foliaceum Blooms In South Carolina: A Multi–Analytical Approach To Identification, Torstein Tengs Dec 2002

Kryptoperidinium Foliaceum Blooms In South Carolina: A Multi–Analytical Approach To Identification, Torstein Tengs

Dr. Torstein Tengs

Observations following the discovery of Kryptoperidinium foliaceum blooms in South Carolina (SC), USA, suggest that a multi-analytical approach, using a standard, minimal set of criteria, should be adopted for determining dinoflagellate species identity and taxonomic placement. A combination of morphological, molecular, and biochemical analyses were used to determine the identity of this “red tide” dinoflagellate, first documented in SC waters in the spring of 1998. Results from thecal plate tabulations (based on scanning electron and epifluorescence microscopy), gene sequence data, species-specific PCR probe assays, and microalgal pigment profiles were analyzed and compared to reference cultures of K. foliaceum. Comparative data …


Monitoring Pv In Corn And Soybean Oils By Nir Spectroscopy, Gülgün (Yildiz) Tiryaki Nov 2002

Monitoring Pv In Corn And Soybean Oils By Nir Spectroscopy, Gülgün (Yildiz) Tiryaki

Dr. Gülgün YILDIZ TIRYAKI

Gülgün Yildiz a, b, Randy L. Wehling*,a, and Susan L. Cuppett a a: Department of Food Science and Technology, University of Nebraska, Lincoln, Nebraska 68583-0919, and b: Olive Culture Research Institute, Universite Cd. No. 43, Bornova/Izmir, Turkey 3500. NIR spectroscopy was used successfully in our laboratory to monitor oxidation levels in vegetable oils. Calibration models were developed to measure PV in both soy and corn oils, using partial least squares (PLS) regression and forward stepwise multiple linear regression, from NIR transmission spectra. PV can be measured successfully in both corn and soy oils using a single calibration. The most successful …


Cutting Edge: Persistent Viral Infection Prevents Tolerance Induction And Escapes Immune Control Following Cd28/Cd40 Blockade-Based Regimen, Thandi M. Onami, M. A. Williams, A. B. Adams, M. M. Durham, T. C. Pearson, R. Ahmed, C. P. Larsen Nov 2002

Cutting Edge: Persistent Viral Infection Prevents Tolerance Induction And Escapes Immune Control Following Cd28/Cd40 Blockade-Based Regimen, Thandi M. Onami, M. A. Williams, A. B. Adams, M. M. Durham, T. C. Pearson, R. Ahmed, C. P. Larsen

Thandi M. Onami

A continuing concern with CD28 and/or CD40 blockade-based strategies to induce tolerance and mixed chimerism is their potential to disrupt protective immunity to preexisting infections. In this report, we find that preexisting persistent infection with lymphocytic choriomeningitis virus (LCMV) clone 13 prevents the induction of tolerance, mixed chimerism, and donor-reactive T cell deletion. Mice continue to be refractory to tolerance induction even after viremia has been resolved and virus is present only at very low levels in peripheral tissues. Conversely, we find that the full tolerance regimen, or costimulation blockade alone, specifically inhibits already ongoing antiviral immune responses, leading to …


Short Communication: Relationship Between Body Growth And Mammary Development In Dairy Heifers, L F. Silva, M J. Vandehaar, Brian K. Whitlock, R P. Radcliff, H A. Tucker Oct 2002

Short Communication: Relationship Between Body Growth And Mammary Development In Dairy Heifers, L F. Silva, M J. Vandehaar, Brian K. Whitlock, R P. Radcliff, H A. Tucker

Brian K Whitlock, PhD, DVM, DACT

Our objective was to determine if prepubertal rate of body weight (BW) gain, independent of diet, was related to mammary development of dairy heifers. Data from two studies recently conducted at Michigan State University were used to identify factors, within a dietary treatment group, that would account for variation in first lactation milk production or amount of mammary parenchymal DNA at the time of puberty. Factors analyzed for variation in milk production during first lactation were: postpartum BW, prepubertal BW gain, gestational BW gain, postpartum BW gain, body condition score (BCS) at breeding, and BCS at calving. Factors analyzed for …


Pmr6, A Pectate Lyase–Like Gene Required For Powdery Mildew Susceptibility In Arabidopsis, John P. Vogel, Ted K. Raab, Celine Schiff, Shauna C. Somerville Sep 2002

Pmr6, A Pectate Lyase–Like Gene Required For Powdery Mildew Susceptibility In Arabidopsis, John P. Vogel, Ted K. Raab, Celine Schiff, Shauna C. Somerville

Ted K. Raab

The plant genes required for the growth and reproduction of plant pathogens are largely unknown. In an effort to identify these genes, we isolated Arabidopsis mutants that do not support the normal growth of the powdery mildew pathogen Erysiphe cichoracearum. Here, we report on the cloning and characterization of one of these genes, PMR6. PMR6 encodes a pectate lyase-like protein with a novel C-terminal domain. Consistent with its predicted gene function, mutations in PMR6 alter the composition of the plant cell wall, as shown by Fourier transform infrared spectroscopy. pmr6-mediated resistance requires neither salicylic acid nor the ability to perceive …


Molecular Cloning And Characterization Of A Novel Mouse Macrophage C-Type Lectin, Mmgl2, Which Has A Distinct Carbohydrate Specificity From Mmgl1, Thandi M. Onami, M. Tsuiji, M. Fujimori, Y. Ohashi, N. Higashi, S. M. Hendrick, T. Irimura Aug 2002

Molecular Cloning And Characterization Of A Novel Mouse Macrophage C-Type Lectin, Mmgl2, Which Has A Distinct Carbohydrate Specificity From Mmgl1, Thandi M. Onami, M. Tsuiji, M. Fujimori, Y. Ohashi, N. Higashi, S. M. Hendrick, T. Irimura

Thandi M. Onami

A novel mouse macrophage galactose-type C-type lectin 2 (mMGL2) was identified by BLAST analysis of expressed sequence tags. The sequence of mMGL2 is highly homologous to the mMGL, which should now be called mMGL1. The open reading frame of mMGL2 contains a sequence corresponding to a type II transmembrane protein with 332 amino acids having a single extracellular C-type lectin domain. The 3'-untranslated region included long terminal repeats of mouse early transposon. The Mgl2 gene was cloned from a 129/SvJ mouse genomic library and sequenced. The gene spans 7,136 base pairs and consists of 10 exons, which is similar to …


No Difference Found In Ribosomal Dna Sequences From Physiologically Diverse Clones Of Karenia Brevis (Dinophyceae) From The Gulf Of Mexico, Torstein Tengs Jul 2002

No Difference Found In Ribosomal Dna Sequences From Physiologically Diverse Clones Of Karenia Brevis (Dinophyceae) From The Gulf Of Mexico, Torstein Tengs

Dr. Torstein Tengs

Maximum growth rate and toxin content were significantly different among five strains of Karenia brevis isolated from Texas and Florida when grown under identical culture conditions. Sequence analysis of the 18S rRNA gene and internal transcribed spacer (ITS) regions revealed, however, that all five strains were identical. Consequently, a clear genetic basis for physiological variability among various geographical isolates of K. brevis from the Gulf of Mexico could not be assessed using these genetic markers. Both the ITS and 18S rRNA regions may be useful in species-specific probe selection. At the intra-specific level, however, an alternative marker will be needed …


Generation Of Mice Deficient For Macrophage Galactose- And N-Acetylgalactosamine-Specific Lectin: Limited Role In Lymphoid And Erythroid Homeostasis And Evidence For Multiple Lectins, Thandi M. Onami, M. Y. Lin, D. M. Page, S. A. Reynolds, C. D. Katayama, J. D. Marth, T. Irimura, A. Varki, N. Varki, S. M. Hedrick Jul 2002

Generation Of Mice Deficient For Macrophage Galactose- And N-Acetylgalactosamine-Specific Lectin: Limited Role In Lymphoid And Erythroid Homeostasis And Evidence For Multiple Lectins, Thandi M. Onami, M. Y. Lin, D. M. Page, S. A. Reynolds, C. D. Katayama, J. D. Marth, T. Irimura, A. Varki, N. Varki, S. M. Hedrick

Thandi M. Onami

Macrophage receptors function in pattern recognition for the induction of innate immunity, in cellular communication to mediate the regulation of adaptive immune responses, and in the clearance of some glycosylated cells or glycoproteins from the circulation. They also function in homeostasis by initiating the engulfment of apoptotic cells. Evidence has suggested that macrophage receptors function to recognize cells that are destined for programmed cell death but not yet overtly apoptotic. We have examined the function of a macrophage receptor specific for unsialylated glycoproteins, known as the mouse macrophage galactose- and N-acetylgalactosamine-specific lectin (mMGL) (Ii et al., J. Biol. Chem. 265:11295-11298, …


Effect Of Dietary Protein On Prepubertal Mammary Development In Rapidly Growing Dairy Heifers, Brian K. Whitlock, M J. Vandehaar, L F. Silva, H A. Tucker Jun 2002

Effect Of Dietary Protein On Prepubertal Mammary Development In Rapidly Growing Dairy Heifers, Brian K. Whitlock, M J. Vandehaar, L F. Silva, H A. Tucker

Brian K Whitlock, PhD, DVM, DACT

The objective was to determine whether increased dietary protein would enhance mammary development in prepubertal heifers fed for rapid body growth (1.2 kg/d). Fifty-four Holstein heifers (weighing approximately 134 kg) were assigned to one of three treatments. Heifers were fed a total mixed ration with metabolizable energy at 2.85 Mcal/kg and metabolizable protein at low, standard, or high concentrations (37, 41, or 44 g/Mcal of metabolizable energy, respectively) from 3.5 mo of age until slaughter at approximately 46 d after puberty. Heifers fed low, standard, and high protein gained 1130, 1170, and 1180 g/d, respectively. Dietary protein did not affect …


Dynamic Regulation Of T Cell Immunity By Cd43, Thandi M. Onami, L. E. Harrington, M. A. Williams, M. Galvan, C. P. Larsen, T. C. Pearson, N. Manjunath, L. G. Baum, B. D. Pearce, R. Ahmed Jun 2002

Dynamic Regulation Of T Cell Immunity By Cd43, Thandi M. Onami, L. E. Harrington, M. A. Williams, M. Galvan, C. P. Larsen, T. C. Pearson, N. Manjunath, L. G. Baum, B. D. Pearce, R. Ahmed

Thandi M. Onami

During a viral response, Ag-specific effector T cells show dramatically increased binding by the mAb 1B11 and the lectin peanut agglutinin (PNA). We investigated the contribution of CD43 expression to 1B11 and PNA binding as well as its role in generation and maintenance of a CD8 T cell response. Analysis of CD43(-/-) mice revealed no increased 1B11 binding and reduced PNA binding on virus-specific CD8 T cells from -/- mice compared with +/+ mice. Furthermore, we examined the role of CD43 in the kinetics of an immune response. We show that CD43 expression modestly effects generation of a primary virus-specific …


Problems With Developmental Stability In Two Rodent Species From Chornobyl, Taras K. Oleksyk, Michael H. Smith, Sergiy P. Gashchak, James M. Novak, James R. Purdue Jan 2002

Problems With Developmental Stability In Two Rodent Species From Chornobyl, Taras K. Oleksyk, Michael H. Smith, Sergiy P. Gashchak, James M. Novak, James R. Purdue

James M. Novak

Changes in developmental patterns are some of the most important effects that may be observed at radioactively contaminated sites like those at Chornobyl. Developmental instability may arise from the interactions between an organism's genotype and its environment and be manifested as deviant morphology. Fluctuating asymmetry (FA) is a measure of deviations from the expected bilateral symmetry of the body. Our objective was to test for differences in FA in two rodent species (Apodemus flavicollis and Clethrionomys glareolus) that live in habitats suirounding the failed Chornobyl reactor. Rodents were collected from four different locations (two contaminated and two reference sites), and …


Scarification Technique Affects Germination Of Stylisma Pickeringii (Patterson Bindweed ), An Illinois Endangered Plant, Henry R. Owen, Brent L. Todd, Janice M. Coons Jan 2002

Scarification Technique Affects Germination Of Stylisma Pickeringii (Patterson Bindweed ), An Illinois Endangered Plant, Henry R. Owen, Brent L. Todd, Janice M. Coons

Henry R. Owen

The seed coat of Stylistna pickeringii (Torr.) Gray var. pattersoni (Patterson bindweed), an endangered species of Illinois sand prairies, inhibits water uptake and seed germination. The purpose of this research was to find an effective and efficient way to scarify seeds of S. pickeringii to aid reintroduction into its natural habitat. Seeds were collected from sandy areas close to the Illinois River near Snicarte (Mason Co.), Illinois during the summers of 1998 and 1999. Experiments were conducted to determine the best scarification techniques (basal cut, sandpaper shakes, sulfuric acid, sand shakes and sonication). Initially, each technique was evaluated by scarifYing …


Cloning Of Plants, Henry R. Owen Jan 2002

Cloning Of Plants, Henry R. Owen

Henry R. Owen

No abstract provided.


Language Plasticity Revealed By Electroencephalogram Mapping, Armando F. Rocha, Flávia B. Foz Jan 2002

Language Plasticity Revealed By Electroencephalogram Mapping, Armando F. Rocha, Flávia B. Foz

Armando F Rocha

Reasoning is the result of the computations made by intelligent systems, for instance those in the brain. It is not an abstract concept because calculations performed by computations are very concrete transactions among the different central processing unit components. Entropy measurements are proposed here to disclose the plasticity of the cerebral processing associated with language comprehension in video game playing. It is also assumed that entropy may be evaluated from the correlation coefficients obtained for the game event-related activity calculated for the different electroencephalogram derivations in the 10/20 system. The brain mapping derived from these entropy measurements clearly demonstrates the …


Self Reliant Agriculture For Arid Lands, David A. Bainbridge Jan 2002

Self Reliant Agriculture For Arid Lands, David A. Bainbridge

David A Bainbridge

More than a billion people face the challenge of supporting themselves in the world's arid lands. Much can be improved by refining and adopting the best traditional practices and crops from around the world. The lessons learned can also help develop new and improved agronomic practices and crops.


Discovery Of The Toxic Dinoflagellate Pfiesteria In Northern European Waters, Torstein Tengs Jan 2002

Discovery Of The Toxic Dinoflagellate Pfiesteria In Northern European Waters, Torstein Tengs

Dr. Torstein Tengs

Several dinoflagellate strains of the genus Pfiesteria were isolated by culturing techniques from sediment samples taken in the Oslofjord region of Norway. Pfiesteria piscicida, well known as a fish killer from the Atlantic coast of America, was identified by genetic methods and light microscopy. The related species Pfiesteria shumwayae was attracted from the sediment by the presence of fish, and has proved toxic. This present survey demonstrates the wide distribution of these potentially harmful species, but so far they have not been connected with fish kills in Europe.


Galactose-Binding Lectin From The Seeds Of Champedak (Artocarpus Integer): Sequences Of Its Subunits And Interactions With Human Serum O-Glycosylated Glycoproteins, Onn Haji Hashim Jan 2002

Galactose-Binding Lectin From The Seeds Of Champedak (Artocarpus Integer): Sequences Of Its Subunits And Interactions With Human Serum O-Glycosylated Glycoproteins, Onn Haji Hashim

Onn Haji Hashim

Our group has previously reported the isolation, partial characterisation, and application of a Galbeta1-3GalNAc- and IgA1-reactive lectin from the seeds of champedak (Artocarpus integer). In the present study, we have subjected the purified lectin to reverse-phase high performance liquid chromatography and sequenced its subunits. Determination of the N-terminal sequence of the first 47 residues of the large subunit demonstrated at least 95% homology to the N-terminal sequence of the alpha chains of a few other galactose-binding Artocarpus lectins, The two smaller subunits of the lectin, each comprised of 21 amino acid residues, demonstrated minor sequence variability. Their sequences were generally …


Influence Of An Extreme High Water Event On Survival, Reproduction, And Distribution Of Snail Kites In Florida, Usa, Robert Bennetts, Wiley Kitchens, Victoria Dreitz Jan 2002

Influence Of An Extreme High Water Event On Survival, Reproduction, And Distribution Of Snail Kites In Florida, Usa, Robert Bennetts, Wiley Kitchens, Victoria Dreitz

Victoria Dreitz

No abstract provided.


Evaluation Of Antemortem Sampling To Estimate Chronic Wasting Disease Prevalence In Free-Ranging Mule Deer, Lisa Wolfe, Mary Conner, T Baker, Victoria Dreitz, Kenneth Burnham, Elizabeth Williams, N T. Hobbs, Michael Miller Jan 2002

Evaluation Of Antemortem Sampling To Estimate Chronic Wasting Disease Prevalence In Free-Ranging Mule Deer, Lisa Wolfe, Mary Conner, T Baker, Victoria Dreitz, Kenneth Burnham, Elizabeth Williams, N T. Hobbs, Michael Miller

Victoria Dreitz

No abstract provided.


Athletes' Use Of Imagery In The Off-Season, Jennifer Cumming, Craig Hall Jan 2002

Athletes' Use Of Imagery In The Off-Season, Jennifer Cumming, Craig Hall

Jennifer Cumming

The purpose of this study was to examine the influence of competitive level on an athletes’ use of imagery in the off-season, and to examine whether their use of imagery was related to their physical and technical preparation. Three hundred and twenty-four regional, provincial, and national level athletes were recruited to participate in this study from 10 different sports. Participants were asked to complete a modified version of the Sport Imagery Questionnaire (SIQ; Hall, Mack, Paivio, & Hausenblas, 1998) that was designed to reflect an athletes’ use of imagery in the off-season. MANOVAs indicated that competitive level differences existed in …


Foraging Ecology And Depredation Management Of Great Blue Herons At Mississippi Catfish Farms, James F. Glahn, Brian S. Dorr, John B. Harrell, Lester Khoo Jan 2002

Foraging Ecology And Depredation Management Of Great Blue Herons At Mississippi Catfish Farms, James F. Glahn, Brian S. Dorr, John B. Harrell, Lester Khoo

Brian S Dorr

No abstract provided.


Newcomers "Invade" The Field Of Invasion Ecology: Question The Field's Future, Mark Davis, K Thompson Jan 2002

Newcomers "Invade" The Field Of Invasion Ecology: Question The Field's Future, Mark Davis, K Thompson

Mark Davis

No abstract provided.


A Cladistic Analysis Of The Cyphophthalmid Genera (Opiliones, Cyphophthalmi), Sarah Boyer, G. Giribet Jan 2002

A Cladistic Analysis Of The Cyphophthalmid Genera (Opiliones, Cyphophthalmi), Sarah Boyer, G. Giribet

Sarah Boyer

No abstract provided.


Variability Of Placental Expression Of Cyclin E Low Molecular Weight Variants, A Bukovsky, Maria Cekanova Ms, Rndr, Phd, Mr Caudle, J Wimalasena, Js Foster, Ja Keenan, Rf Elder Jan 2002

Variability Of Placental Expression Of Cyclin E Low Molecular Weight Variants, A Bukovsky, Maria Cekanova Ms, Rndr, Phd, Mr Caudle, J Wimalasena, Js Foster, Ja Keenan, Rf Elder

Maria Cekanova MS, RNDr, PhD

No abstract provided.


Ethological Causes And Consequences Of The Stress Response, Neil Greenberg, James A. Carr, Cliff H. Summers Jan 2002

Ethological Causes And Consequences Of The Stress Response, Neil Greenberg, James A. Carr, Cliff H. Summers

Neil Greenberg

Stress involves real or perceived changes within an organism or in the environment that activate an organism’s attempts to cope by means of evolutionarily ancient neural and endocrine mechanisms. Responses to acute stressors involve catecholamines released in varying proportion at different sites in the sympathetic and central nervous systems. These responses may interact with and be complemented by intrinsic rhythms and responses to chronic or intermittent stressors involving the hypothalamic-pituitary-adrenal axis. Varying patterns of responses to stressors are also affected by an animal=s assessment of their prospects for successful coping. Subsequent central and systemic consequences of the stress response include …


Ethological Aspects Of Stress In A Model Lizard, Anolis Carolinensis, Neil Greenberg Jan 2002

Ethological Aspects Of Stress In A Model Lizard, Anolis Carolinensis, Neil Greenberg

Neil Greenberg

Research on the stress response in reptiles can provide a useful comparative perspective for understanding how the constituent elements of the response can be put into service of diverse behavioral adaptations. A summary of the neural and endocrine causes and consequences of specific behavioral patterns seen in the small diurnal lizard, Anolis carolinensis, has provided a model for the exploration of the dynamics of autonomic and neurohormonal contributions to adaptive behavior. In this species, changes in body color provide indices of the flux of circulating stress relevant hormones, and are seen in situations from spontaneous exploration through agonistic behavior. Furthermore, …


Adaptive Functions Of The Corpus Striatum: The Past And Future Of The R-Complex, Neil Greenberg Jan 2002

Adaptive Functions Of The Corpus Striatum: The Past And Future Of The R-Complex, Neil Greenberg

Neil Greenberg

The basal ganglia is emerging from the shadow cast by the most conspicuous clinical expression of its dysfunction: motor disorders.What is revealed is the nexus of a widely distributed system which functions in integrating action with cognition, motivation, and affect. Prominent among non-motor functions are striatal involvement in building up of sequences of behavior into meaningful, goal-directed patterns and repertoires and the selection of appropriate learned or innate sequences in concert with their possible predictive control. Further, striatum seems involved in declarative and strategic memory (involving intentional recollection and the management of retrieved memories, respectively). Findings from reptile experiments indicate …