Life Sciences Commons^™

Use Of Molecular Probes To Assess Geographic Distribution Of Pfiesteria Species, Torstein Tengs

Dr. Torstein Tengs

We have developed multiple polymerase chain reaction (PCR)-based methods for the detection of Pfiesteria sp. in cultures and environmental samples. More than 2,100 water and sediment samples from estuarine sites of the U.S. Atlantic and gulf coasts were assayed for the presence of Pfiesteria piscicida Steidinger & Burkholder and Pfiesteria shumwayae Glasgow & Burkholder by PCR probing of extracted DNA. Positive results were found in about 3% of samples derived from routine monitoring of coastal waters and about 8% of sediments. The geographic range of both species was the same, ranging from New York to Texas. Pfiesteria spp. are likely …

Classification And Identification Of Pfiesteria And Pfiesteria-Like Species, Torstein Tengs

Dr. Torstein Tengs

Dinoflagellates can be classified both botanically and zoologically; however, they are typically put in the botanical division Pyrrhophyta. As a group they appear most related to the protistan ciliates and apicomplexans at the ultrastructure level. Within the Pyrrhophyta are both unarmored and armored forms of the dominant, motile flagellated stage. Unarmored dinoflagellates do not have thecal or wall plates arranged in specific series, whereas armored species have plates that vary in thickness but are specific in number and arrangement. In armored dinoflagellates, the plate pattern and tabulation is a diagnostic character at the family, subfamily, and even genus levels. In …

Influential Parameters Of Job Stress - A Regression Analysis Of Women Scientists In Indian Agricultural Research System, Aneeja Guttikonda

aneeja guttikonda

No abstract provided.

Invasion Terminology: Should Ecologists Define Their Terms Differently Than Others? No, Not If We Want To Be Of Any Help!, Mark Davis, K Thompson

Mark Davis

No abstract provided.

Visualizing Rhizosphere Chemistry Of Legumes With Mid-Infrared Synchrotron Radiation, Ted K. Raab, Michael C. Martin

Ted K. Raab

A bright synchrotron light source operated by the Lawrence Berkeley National Laboratory served as an external source for infrared (IR) microscopy of plant root microcosms. Mid-IR light from synchrotrons is 2-3 orders of magnitude brighter than conventional sources, providing contrast based on the chemical information in the reflected signal at a spatial resolution near the diffraction-limit of 3-10 microns. In an experiment using plant root microcosms fitted with zinc selenide IR-transmissive windows (50 mm x 20 mm x 1 mm), we describe chemical differences and similarities within the root zone of mung bean (Vigna radiata L.), grown with or without …

Method For Determining Oxidation Of Vegetable Oils By Near-Infrared Spectroscopy, Gulgun (Yildiz) Tiryaki

Dr. Gülgün YILDIZ TIRYAKI

No abstract provided.

Genetic Polymorphism In Gymnodinium Galatheanum Chloroplast Dna Sequences And Development Of A Molecular Detection Assay, Torstein Tengs

Dr. Torstein Tengs

Nuclear and chloroplast-encoded small subunit ribosomal DNA sequences were obtained from several strains of the toxic dinoflagellate Gymnodinium galatheanum . Phylogenetic analyses and comparison of sequences indicate that the chloroplast sequences show a higher degree of sequence divergence than the nuclear homologue. The chloroplast sequences were chosen as targets for the development of a 5'–3' exonuclease assay for detection of the organism. The assay has a very high degree of specificity and has been used to screen environmental water samples from a fish farm where the presence of this dinoflagellate species has previously been associated with fish kills. Various hypotheses …

Genetic Heterogeneity Of White-Tailed Deer: Management Lessons From A Long-Term Study, Michael H. Smith, James M. Novak, J. D. Peles, J. R. Purdue

James M. Novak

Genetic data from a long-term (16-year) study of white-tailed deer (Odocoileus virginianus) on the U.S. Department of Energy's Savannah River Site (SRS) were examined to evaluate spatial and temporal genetic heterogeneity in this species. Based on our analyses of the long-term data set, three major findings emerged, all of which have important implications for management of white-tailed deer: (1) There exists significant spatial genetic heterogeneity in white-tailed deer based on analyses of allozyme frequencies and mtDNA haplotypes. This heterogeneity exists on a much smaller spatial scale than would be expected for such a large and potentially mobile species as 0. …

N-Methyl-D-Aspartate Channel And Consciousness: From Signal Coincidence Detection To Quantum Computing, Armando F. Rocha, Alfredo Pereira Jr

Armando F Rocha

Research on Blindsight, Neglect/Extinction and Phantom limb syndromes, as well as electrical measurements of mammalian brain activity, have suggested the dependence of vivid perception on both incoming sensory information at primary sensory cortex and reentrant information from associative cortex. Coherence between incoming and reentrant signals seems to be a necessary condition for (conscious) perception. General reticular activating system and local electrical synchronization are some of the tools used by the brain to establish coarse coherence at the sensory cortex, upon which biochemical processes are coordinated. Besides electrical synchrony and chemical modulation at the synapse, a central mechanism supporting such a …

Ecocomposite Materials, David A. Bainbridge

David A Bainbridge

Ecocomposite materials combine fibers, resins and natural materials for added strength and durability. These materials can be fully and easily recycled by nature.

Real–Time Pcr Monitoring Of Estuarine Water Samples For Pfiesteria Piscicida, A Dinoflagellate Associated With Fish Kills And Human Illness, Torstein Tengs

Dr. Torstein Tengs

No abstract.

Teaching The Human Dimensions Of Care In The Clinical Settings, William T. Branch Jr. Md, Macp

William T.Branch Jr.MD

Despite repeated calls to emphasize the humanistic dimensions of care during medical education, these are few known techniques for effective teaching of humanism. We describe the barriers that inhibit humanistic teaching and suggest pragmatic teaching methods to overcome such barriers and teach humanistic care in clinical settings. We began by asking participants at a conference on patient-physician communications sponsored by the American Academy on Physician and Patient in June 1998, "What can we do in the patient's presence to improve and teach the human dimensions of care? Please provide one or more examples of approaches you found to be effective." …

The Use Of Red Mangrove As Nesting Substrate For Snail Kites In Florida, Victoria Dreitz, James Duberstein

Victoria Dreitz

No abstract provided.

Population Viability Of The Gulf Of Mexico Sturgeon In The Suwannee River, Florida, William Pine, Michael Allen, Victoria Dreitz

Victoria Dreitz

No abstract provided.

Vasorelaxant Effects Of Ethyl Cinnamate Isolated From Kaempferia Galanga On Smooth Muscles Of The Rat Aorta, Mohd Rais Mustafa, Rozana Othman, Halijah Ibrahim, Mustafa Ali Mohd, Khalijah Awang, Anwar-Ul Hassan Gilani

Mohd Rais Mustafa

From the rhizomes of Kaempferia galanga, ethyl cinnamate (EC) was isolated and its vasorelaxant effect was examined on the rat aorta. EC inhibited the tonic contractions induced by high K+ and phenylephrine (PE) in a concentration-dependent manner, with respective IC50 values of 0.30 ± 0.05 mM and 0.38 ± 0.04 mM. The relaxant effect against PE-induced contractions was greater in the presence of endothelium. Pre-treatment of the aorta with methylene blue and indomethacin significantly reduced the relaxant effect. These results suggest that the inhibitory effects of EC may involve inhibition of Ca2+ influx into vascular cells and release of nitric …

The Cognitive And Motivational Effects Of Imagery Training: A Matter Of Perspective, Jennifer L. Cumming, Diane M. Ste-Marie

Jennifer Cumming

No abstract provided.

El Laboratorio De Cádiz Del Instituto De Investigaciones Pesqueras : ”Arena” Investigadora Del Csic Entre 1957 Y 1966., Enrique Wulff

Enrique Wulff

The aim of this communication is to detail some main images projected by CSIC from its laboratuory in Cádiz, as critical information points from the past. 1957-1966 is the studied period, its fondational time. The indexation of the scientific problems related with the activity of the laboratory makes use of the calculation of transition probabilities. Catch percentages and bathymetric distributions are emoployed to approach the structure of the oceanographic campaigns results. The quantitative enumeration of observed organs and mathematical procedures provide information on the observations in the tunny fisheries. Multivariant bibliometry (factorial and cluster analysis, multiple regression) , and coauthor …

The Unusual Life History Of Alseis Blackiana: A Shade-Persistent Pioneer Tree?, Jason D. Nason, J. W. Dalling, K. Winter, S. P. Hubbell, D. A. Murawski, J. L. Hamrick

Jason D Nason

Classifications of tree species into regeneration guilds or functional groups are often based on a limited number of life history characteristics indicative of requirements for early establishment, with the assumption that these traits are correlated with growth requirements of larger individuals. We tested the validity of this assumption for Alseis blackiana, a common canopy tree in central Panama, by examining its seed germination, seedling growth, and sapling distribution, growth, and mortality. We found that the early life history of Alseis is characteristic of pioneer species: the seed germination rate was much higher in forest gaps than in the forest understory, …

Public Access And Use Of Electronically Archived Data: Ethical Considerations, Mark Davis, D Tilman, S Hobbie, Cl Lehman, Et Al.

Mark Davis

No abstract provided.

Abnormal Expression Of P27kip1 Protein In Levator Ani Muscle Of Aging Women With Pelvic Floor Disorders – A Relationship To The Cellular Differentiation And Degeneration, Antonin Bukovsky, Pleas Copas, Michael R. Caudle, Maria Cekanova Ms, Rndr, Phd, Tamara Dassanayake, Bridgett Asbury, Stuart E. Van Meter, Robert F. Elder, Jeffrey B. Brown, Stephanie B. Cross

Maria Cekanova MS, RNDr, PhD

Background Pelvic floor disorders affect almost 50% of aging women. An important role in the pelvic floor support belongs to the levator ani muscle. The p27/kip1 (p27) protein, multifunctional cyclin-dependent kinase inhibitor, shows changing expression in differentiating skeletal muscle cells during development, and relatively high levels of p27 RNA were detected in the normal human skeletal muscles.

Methods Biopsy samples of levator ani muscle were obtained from 22 symptomatic patients with stress urinary incontinence, pelvic organ prolapse, and overlaps (age range 38–74), and nine asymptomatic women (age 31–49). Cryostat sections were investigated for p27 protein expression and type I (slow …

Protein Targeting And Translocation, Barry D. Bruce, D.A. Phoenix

Barry D. Bruce

No abstract provided.

The Ethics Of Sport: A Reader, Chad R. Carlson

Chad Carlson

No abstract provided.