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Micropropagation Of Mangifera Indica L. Cv. Kurakkan Through Somatic Embryogenesis, Rajesh Pati
Micropropagation Of Mangifera Indica L. Cv. Kurakkan Through Somatic Embryogenesis, Rajesh Pati
Rajesh Pati
Nucellar embryogenesis was induced in Mangifera indica L. cv. Kurakkan, a polyembryonic salt tolerant, dwarfing rootstock. Nucellus tissue excised from 3.5 cm long fruits developed pro-embryonic callus in 19 days of inoculation on modified MS medium supplemented with 4.52μM 2,4- D, 0.05% malt extract and 13.78μM spermidine. Somatic embryogenesis exhibited high frequency (158.33 embryos). However, all the differentiated embryos proliferated on medium having low level of sucrose (4% w/v) and auxin (2.26μM 2,4-D). Most of the proembryonic calli converted into heart shaped and cotyledonary embryos by reducing temperature to 15oC. Somatic embryos were matured on modified MS medium fortified with …