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Lactobacilli Inhibit Shigella Dysenteriae 1 Induced Pro-Inflammatory Response And Cytotoxicity In Host Cells Via Impediment Of Shigella-Host Interactions, Murali Mr, Moorthy G, Niranjali Devaraj S

Murali Malliga Raman

OBJECTIVE: Shigella dysenteriae Type 1 dysentery is a major cause of morbidity and mortality in children from less developed and developing countries. The present study explores the hypothesis that lactobacilli protect the host cell during S. dysenteriae Type 1 infection and its mechanism of action. METHODS: Caco-2 cells incubated for 1h with Lactobacillus rhamnosus or Lactobacillus acidophilus at the multiplicity of infection of 100, either alone or in combination followed by addition of Shigella at the same multiplicity of infection for 5h served as treatment groups. Cells incubated with Shigella without lactobacilli addition served as infected cells. At the end …