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Differential Expression Of Gastric Muc5ac In Colonic Epithelial Cells: Tff-3 Wired Il1Β/Akt Crosstalk Induced Mucosal Immune Response Against Shigella Dysenteriae Infection, Raja Sb, Murali Mr, Devaraj H, Devaraj Sn
Differential Expression Of Gastric Muc5ac In Colonic Epithelial Cells: Tff-3 Wired Il1Β/Akt Crosstalk Induced Mucosal Immune Response Against Shigella Dysenteriae Infection, Raja Sb, Murali Mr, Devaraj H, Devaraj Sn
Murali Malliga Raman
An understanding of the signaling mechanism(s) that regulate the differential expression of gastric mucin MUC5AC in colonic epithelial cells would contribute significantly to investigations of its role in colonic mucosa infected with the bacterial pathogen Shigella dysenteriae. Here we show that S. dysenteriae-Sinduced expression of interleukin-1β upregulates MUC2 expression and the differential expression of MUC5AC. Differential expression of MUC5AC involves crosstalk between interleukin-1β and Akt, whereby the trefoil factor family peptide TFF3 activates Akt by phosphorylation of EGFR. TFF3 also downregulates E-cadherin expression, causing accumulation of β-catenin in the cytosol. Phosphorylation of GSK-3β (inactivated) by activated Akt inhibits ubiquitylation of …
Lactobacilli Inhibit Shigella Dysenteriae 1 Induced Pro-Inflammatory Response And Cytotoxicity In Host Cells Via Impediment Of Shigella-Host Interactions, Murali Mr, Moorthy G, Niranjali Devaraj S
Lactobacilli Inhibit Shigella Dysenteriae 1 Induced Pro-Inflammatory Response And Cytotoxicity In Host Cells Via Impediment Of Shigella-Host Interactions, Murali Mr, Moorthy G, Niranjali Devaraj S
Murali Malliga Raman
OBJECTIVE: Shigella dysenteriae Type 1 dysentery is a major cause of morbidity and mortality in children from less developed and developing countries. The present study explores the hypothesis that lactobacilli protect the host cell during S. dysenteriae Type 1 infection and its mechanism of action. METHODS: Caco-2 cells incubated for 1h with Lactobacillus rhamnosus or Lactobacillus acidophilus at the multiplicity of infection of 100, either alone or in combination followed by addition of Shigella at the same multiplicity of infection for 5h served as treatment groups. Cells incubated with Shigella without lactobacilli addition served as infected cells. At the end …