Open Access. Powered by Scholars. Published by Universities.®
- Keyword
Articles 1 - 8 of 8
Full-Text Articles in Life Sciences
Biosorption Of Heavy Metals By Bacillus Thuringiensis Strain Osm29 Originating From Industrial Effluent Contaminated North Indian Soil, Mohammad Oves
Biosorption Of Heavy Metals By Bacillus Thuringiensis Strain Osm29 Originating From Industrial Effluent Contaminated North Indian Soil, Mohammad Oves
Mohammad Oves
No abstract provided.
Pseudomonas Fluorescens Hk44: Lessons Learned From A Model Whole-Cell Bioreporter With A Broad Application History, Josef Trögl, Archana Chauhan, Steven Ripp, Alice C. Layton, Gabriela Kuncová, Gary S. Sayler
Pseudomonas Fluorescens Hk44: Lessons Learned From A Model Whole-Cell Bioreporter With A Broad Application History, Josef Trögl, Archana Chauhan, Steven Ripp, Alice C. Layton, Gabriela Kuncová, Gary S. Sayler
Gary S. Sayler
Initially described in 1990, Pseudomonas fluorescens HK44 served as the first whole-cell bioreporter genetically endowed with a bioluminescent (luxCDABE) phenotype directly linked to a catabolic (naphthalene degradative) pathway. HK44 was the first genetically engineered microorganism to be released in the field to monitor bioremediation potential. Subsequent to that release, strain HK44 had been introduced into other solids (soils, sands), liquid (water, wastewater), and volatile environments. In these matrices, it has functioned as one of the best characterized chemically-responsive environmental bioreporters and as a model organism for understanding bacterial colonization and transport, cell immobilization strategies, and the kinetics of cellular bioluminescent …
Hslic Fall 2012 Scholarship Committee Report, Ann Jordan
Hslic Fall 2012 Scholarship Committee Report, Ann Jordan
Ann Jordan
No abstract provided.
Desiccation Induces Viable But Non-Culturable Cells In Sinorhizobium Meliloti 1021, Jan A.C. Vriezen Dr., Frans J. Debruijn Dr., Klaus R. Nusslein Dr.
Desiccation Induces Viable But Non-Culturable Cells In Sinorhizobium Meliloti 1021, Jan A.C. Vriezen Dr., Frans J. Debruijn Dr., Klaus R. Nusslein Dr.
Jan A.C. Vriezen Dr.
Sinorhizobium meliloti is a microorganism commercially used in the production of e.g. Medicago sativa seed inocula. Many inocula are powder-based and production includes a drying step. Although S. meliloti survives drying well, the quality of the inocula is reduced during this process. In this study we determined survival during desiccation of the commercial strains 102F84 and 102F85 as well as the model strain USDA1021. The survival of S. meliloti 1021 was estimated during nine weeks at 22% relative humidity. We found that after an initial rapid decline of colony forming units, the decline slowed to a steady 10-fold reduction in …
Differential Expression Of Gastric Muc5ac In Colonic Epithelial Cells: Tff-3 Wired Il1Β/Akt Crosstalk Induced Mucosal Immune Response Against Shigella Dysenteriae Infection, Raja Sb, Murali Mr, Devaraj H, Devaraj Sn
Differential Expression Of Gastric Muc5ac In Colonic Epithelial Cells: Tff-3 Wired Il1Β/Akt Crosstalk Induced Mucosal Immune Response Against Shigella Dysenteriae Infection, Raja Sb, Murali Mr, Devaraj H, Devaraj Sn
Murali Malliga Raman
An understanding of the signaling mechanism(s) that regulate the differential expression of gastric mucin MUC5AC in colonic epithelial cells would contribute significantly to investigations of its role in colonic mucosa infected with the bacterial pathogen Shigella dysenteriae. Here we show that S. dysenteriae-Sinduced expression of interleukin-1β upregulates MUC2 expression and the differential expression of MUC5AC. Differential expression of MUC5AC involves crosstalk between interleukin-1β and Akt, whereby the trefoil factor family peptide TFF3 activates Akt by phosphorylation of EGFR. TFF3 also downregulates E-cadherin expression, causing accumulation of β-catenin in the cytosol. Phosphorylation of GSK-3β (inactivated) by activated Akt inhibits ubiquitylation of …
Anaerobic Oxidation Of Methane At Different Temperature Regimes In Guaymas Basin Hydrothermal Sediments, Jennifer F. Biddle, Zena Cardman, Howard Mendlovitz, Daniel B. Albert, Karen G. Lloyd, Karen Lloyd, Antje Boetius, Andreas Teske
Anaerobic Oxidation Of Methane At Different Temperature Regimes In Guaymas Basin Hydrothermal Sediments, Jennifer F. Biddle, Zena Cardman, Howard Mendlovitz, Daniel B. Albert, Karen G. Lloyd, Karen Lloyd, Antje Boetius, Andreas Teske
Karen Lloyd
Anaerobic oxidation of methane (AOM) was investigated in hydrothermal sediments of Guaymas Basin based on d13C signatures of CH4, dissolved inorganic carbon and porewater concentration profiles of CH4 and sulfate. Cool, warm and hot in-situ temperature regimes (15–20 1C, 30–35 1C and 70–95 1C) were selected from hydrothermal locations in Guaymas Basin to compare AOM geochemistry and 16S ribosomal RNA (rRNA), mcrA and dsrAB genes of the microbial communities. 16S rRNA gene clone libraries from the cool and hot AOM cores yielded similar archaeal types such as Miscellaneous Crenarchaeotal Group, Thermoproteales and anaerobic methane-oxidizing archaea (ANME)-1; some of the ANME-1 …
Archaea Of The Miscellaneous Crenarchaeotal Group (Mcg) Are Abundant, Diverse, And Widespread In Marine Sediments, Kyoko Kubo, Karen Lloyd, Jennifer F. Biddle, Rudolf Amann, Andreas Teske, Katrin Knittel
Archaea Of The Miscellaneous Crenarchaeotal Group (Mcg) Are Abundant, Diverse, And Widespread In Marine Sediments, Kyoko Kubo, Karen Lloyd, Jennifer F. Biddle, Rudolf Amann, Andreas Teske, Katrin Knittel
Karen Lloyd
Members of the highly diverse Miscellaneous Crenarchaeotal Group (MCG) are globally distributed in various marine and continental habitats. In this study, we applied a polyphasic approach (rRNA slot blot hybridization, quantitative PCR (qPCR) and catalyzed reporter deposition FISH) using newly developed probes and primers for the in situ detection and quantification of MCG crenarchaeota in diverse types of marine sediments and microbial mats. In general, abundance of MCG (cocci, 0.4 lm) relative to other archaea was highest (12–100%) in anoxic, low-energy environments characterized by deeper sulfate depletion and lower microbial respiration rates (P¼0.06 for slot blot and P¼0.05 for qPCR). …
Ameliorating Risk: Culturable And Metagenomic Monitoring Of The 14 Year Decline Of A Genetically Engineered Microorganism At A Bioremediation Field Site, A. C. Layton, A. E. Smartt, A. Chauhan, S. Ripp, D. E. Williams, W. Burton, S. Moser, J. Phillips, A. V. Palumbo, Gary S. Sayler
Ameliorating Risk: Culturable And Metagenomic Monitoring Of The 14 Year Decline Of A Genetically Engineered Microorganism At A Bioremediation Field Site, A. C. Layton, A. E. Smartt, A. Chauhan, S. Ripp, D. E. Williams, W. Burton, S. Moser, J. Phillips, A. V. Palumbo, Gary S. Sayler
Gary S. Sayler
In 1996, the first EPA sanctioned release of a recombinant microbe (Pseudomonas fluorescens HK44) into the subsurface soil environment was initiated in a replicated semi-contained array of soil lysimeters. With an aim to access the survivability/environmental fate of HK44, soil sampling was performed 14 years post release. Although after extensive sampling culturable HK44 cells were not found, qPCR and metagenomic analyses indicated that genetic signatures of HK44 cells still persisted in the soils, with genes diagnostic for the bioluminescent transposon carried by strain HK44 (luxA and tetA) being found at low concentrations (< 5000 copies/g). Additionally, metagenome analysis of lysimeter 2 using amplicon pyrosequencing showed that Burkholderia was more abundant in the sample extracted before storage at 4°C than after storage at 4°C (79% and 5.6% Burkholderia sequences, respectively). http://www.omicsonline.org/2155-6199/2155-6199-S1-009.php?aid=5830 doi:10.4172/2155-6199.S1-009