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Capsular Serotyping Of Pasteurella Multocida From Various Animal Hosts - A Comparison Of Phenotypic And Genotypic Methods, Thong Kwai Lin
Capsular Serotyping Of Pasteurella Multocida From Various Animal Hosts - A Comparison Of Phenotypic And Genotypic Methods, Thong Kwai Lin
Thong Kwai Lin
One hundred and fourteen strains of Pasteurella multocida were isolated from different domestic animals species (cattle, buffalo, sheep, goat, pig, rabbit, dog, cat), avian species (chicken, duck, turkey) and wild animals (deer, tiger, orang utan, marmoset). The serogroups of P multocida were determined by both conventional capsular serotyping and a multiplex PCR assay targeting specific capsular genes. Based on the conventional serotyping method, the 114 strains of P multocida were subtyped into 55 species-specific (untypeable strains) P. multocida, 15 serogroup A, 23 serogroup B and 21 serogroup D. Based on the multiplex PCR assay on the specific capsular genes associated …
Antimicrobial Susceptibility Profiling And Genomic Diversity Of Multidrug-Resistant Acinetobacter Baumannii Isolates From A Teaching Hospital In Malaysia, Thong Kwai Lin
Thong Kwai Lin
The resistance phenotypes and genomic diversity of 185 Acinetobacter baumannii isolates obtained from the intensive care unit (ICU) of a local teaching hospital in Kuala Lumpur from 2006 to 2009 were determined using antimicrobial susceptibility testing and pulsed-field gel electrophoresis (PFGE). Antibiogram analyses showed that the isolates were fully resistant to beta-lactam antimicrobials and had high resistance rates to the other antimicrobial agents tested. However, the isolates were susceptible to polymyxin B. Resistance to cefoperazone/sulbactam was only detected in strains isolated from 2007 to 2009. Some environmental isolates and an isolate from the hands of a healthcare worker (HCW) had …
Further Evaluation Of A Multiplex Pcr For Differentiation Of Salmonella Paratyphi A From Other Salmonellae, Thong Kwai Lin
Further Evaluation Of A Multiplex Pcr For Differentiation Of Salmonella Paratyphi A From Other Salmonellae, Thong Kwai Lin
Thong Kwai Lin
Salmonella enterica serovar Paratyphi A is a causative agent of paratyphoid fever. The clinical syndrome caused by paratyphoid fever overlaps with other febrile illnesses and cannot be distinguished from typhoid fever. Conventional methods used for diagnosis are time consuming, costly, and labor-intensive. We evaluated the specificity, sensitivity, and application of a multiplex polymerase chain reaction (PCR) previously developed by the method (Ou, H.Y, Teh, C.S.J., Thong, K.L., et al., J. Mol. Diagn., 9, 624-630, 2007) using 6 S. Paratyphi A, 22 S. Typhi, and 85 other Salmonella serovars as well as 36 non-Salmonella strains. The detection limit of the multiplex …