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Investigation Of Murine Spleen As A Niche For Hematopoiesis., Jonathan Tan, Helen O'Neill
Investigation Of Murine Spleen As A Niche For Hematopoiesis., Jonathan Tan, Helen O'Neill
Jonathan Tan
Background Spleen as a lymphoid tissue is specialized for monitoring blood and mounting immunity against blood-borne antigens. Antigen-presenting cells present in spleen commonly develop from bone marrow-derived precursors that enter blood circulation. However, a distinct splenic myeloid antigen-presenting cell subset described in this laboratory, namely “dendritic-like cells” (L-DC), has been hypothesized not to share a bone marrow origin. Methods In this study, the presence of endogenous hematopoietic progenitors in spleen was investigated by transplanting intact spleen into allotype-distinct recipients and monitoring development of progeny cells in grafted tissues. Results Successful engraftment of donor spleens was achieved for up to 4 …
Investigation Of Murine Spleen As A Niche For Hematopoiesis., Jonathan Tan, Helen O'Neill
Investigation Of Murine Spleen As A Niche For Hematopoiesis., Jonathan Tan, Helen O'Neill
Helen O'Neill
Background Spleen as a lymphoid tissue is specialized for monitoring blood and mounting immunity against blood-borne antigens. Antigen-presenting cells present in spleen commonly develop from bone marrow-derived precursors that enter blood circulation. However, a distinct splenic myeloid antigen-presenting cell subset described in this laboratory, namely “dendritic-like cells” (L-DC), has been hypothesized not to share a bone marrow origin. Methods In this study, the presence of endogenous hematopoietic progenitors in spleen was investigated by transplanting intact spleen into allotype-distinct recipients and monitoring development of progeny cells in grafted tissues. Results Successful engraftment of donor spleens was achieved for up to 4 …
Regulatory Elements Of Xenopus Col2a1 Drive Cartilaginous Gene Expression In Transgenic Frogs, Ryan Kerney, Brian K. Hall, James Hanken
Regulatory Elements Of Xenopus Col2a1 Drive Cartilaginous Gene Expression In Transgenic Frogs, Ryan Kerney, Brian K. Hall, James Hanken
Ryan Kerney
This study characterizes regulatory elements of collagen 2α1 (col2a1) in Xenopus that enable transgene expression in cartilage-forming chondrocytes. The reporters described in this study drive strong cartilage-specific gene expression, which will be a valuable tool for further investigations of Xenopus skeletal development. While endogenous col2a1 mRNA is expressed in many embryonic tissues, its expression becomes restricted to tadpole and adult chondrocytes. This chondrocyte-specific expression is recapitulated by col2a1 reporter constructs, which were tested through I-SceI meganuclease-mediated transgenesis. These constructs contain a portion of the Xenopus tropicalis col2a1 intron, which aligns to a cartilage-specific intronic enhancer that has been well characterized …