Spiroplasma Lineolae Sp. Nov., From The Horsefly Tabanus Lineola (Diptera : Tabanidae), Frank E. French, Robert F. Whitcomb, Joseph G. Tully, Patricia Carle, Joseph M. Bové, Roberta B. Henegar, Jean R. Adams, Gail E. Gasparich, David L. Williamson
Sep 1997
Spiroplasma Lineolae Sp. Nov., From The Horsefly Tabanus Lineola (Diptera : Tabanidae), Frank E. French, Robert F. Whitcomb, Joseph G. Tully, Patricia Carle, Joseph M. Bové, Roberta B. Henegar, Jean R. Adams, Gail E. Gasparich, David L. Williamson
Gail Gasparich
Spiroplasma strain TALS-2T from the viscera of the striped horsefly, Tabanus lineola, collected in Georgia was serologically distinct from other Spiroplasma species, groups, putative groups, and subgroups. Light and electron microscopy of cells of strain TALS-2T revealed helical motile cells surrounded only by a single cytoplasmic membrane. The organism grew in M1D and SP-4 liquid media. Growth also occurred in 1% serum fraction medium and in conventional horse serum medium. Growth in liquid media was serum dependent. The strain passed through 220-nm filter pores, but was retained in filters with 100-nm pores. The optimum temperature for growth was 30°C. Multiplication …
Spiroplasma Chrysopicola Sp. Nov., Spiroplasma Gladiatoris Sp. Nov., Spiroplasma Helicoides Sp. Nov., And Spiroplasma Tabanidicola Sp. Nov., From Tabanid (Diptera: Tabanidae) Flies, Robert F. Whitcomb, Frank E. French, Joseph G. Tully, Gail E. Gasparich, David L. Rose, Patricia Carle, Joseph M. Bove, Roberta B. Henegar, Meghnad Konai, Kevin J. Hackett, Jean R. Adams, Truman B. Clark, David L. Williamson
Jun 1997
Spiroplasma Chrysopicola Sp. Nov., Spiroplasma Gladiatoris Sp. Nov., Spiroplasma Helicoides Sp. Nov., And Spiroplasma Tabanidicola Sp. Nov., From Tabanid (Diptera: Tabanidae) Flies, Robert F. Whitcomb, Frank E. French, Joseph G. Tully, Gail E. Gasparich, David L. Rose, Patricia Carle, Joseph M. Bove, Roberta B. Henegar, Meghnad Konai, Kevin J. Hackett, Jean R. Adams, Truman B. Clark, David L. Williamson
Gail Gasparich
Four spiroplasma strains, DF-1T, TG-1T, TABS-2T, and TAUS-1T, all of which were isolated from deerflies or horseflies (Diptera: Tabanidae), were serologically distinct from previously described spiroplasma species, groups, and subgroups. Strain DF-1Toriginated from a Maryland deerfly (Chrysops sp.); strain TG-1Twas isolated from a Maryland horsefly (Tabanus gladiator); strain TAUS-1Toriginated from a member of the Tabanus abdominalis-limbatinevris complex of horseflies collected in Maryland; and strain TABS-2Twas isolated from a horsefly (Tabanus abactor) collected in Oklahoma. Cells of all of the strains appeared to be helical and motile when they were examined by dark-field microscopy. Cells of strain DF-1Tgrowing in M1D medium …
The Spiroplasma Motility Inhibition Test, A New Method For Determining Intraspecific Variation Among Colorado Potato Beetle Spiroplasmas, Kevin J. Hackett, J. J. Lipa, G. E. Gasparich, D. E. Lynn, M. Konai, M. Camp, R. F. Whitcomb
Dec 1996
The Spiroplasma Motility Inhibition Test, A New Method For Determining Intraspecific Variation Among Colorado Potato Beetle Spiroplasmas, Kevin J. Hackett, J. J. Lipa, G. E. Gasparich, D. E. Lynn, M. Konai, M. Camp, R. F. Whitcomb
Gail Gasparich
The Colorado potato beetle, Leptinotarsa decemlineata, is a major holarctic pest of solanaceous crops. Presumably, this insect spread from Solanum species in central America to the Mexican plateau, and this was followed by multiple invasions of North America and Europe. Attempts are being made to control this beetle by using a genetically modified spiroplasma that occurs naturally in its gut. In the current study, spiroplasmas isolated from beetles collected in North America and Poland exhibited serologic (spiroplasma motility inhibition test) and genomic (restriction fragment length polymorphism) profiles that suggest that there were multiple spiroplasma introductions. Two serovars were identified; one …
