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Pfcg2, A Plasmodium Falciparum Protein Peripherally Associated With The Parasitophorous Vacuolar Membrane, Is Expressed In The Period Of Maximum Hemoglobin Uptake And Digestion By Trophozoites, Roland A. Cooper, Janni Papakrivos, Kristen D. Lane, Hisashi Fujioka, Klaus Lingelbach, Thomas E. Wellems
Pfcg2, A Plasmodium Falciparum Protein Peripherally Associated With The Parasitophorous Vacuolar Membrane, Is Expressed In The Period Of Maximum Hemoglobin Uptake And Digestion By Trophozoites, Roland A. Cooper, Janni Papakrivos, Kristen D. Lane, Hisashi Fujioka, Klaus Lingelbach, Thomas E. Wellems
Roland A. Cooper
A Plasmodium falciparum gene closely linked to the chloroquine resistance locus encodes PfCG2, a predicted 320-330kDa protein. In the parasitized erythrocyte, PfCG2 expression rises sharply in the trophozoite stage and is detected in electron-dense patches along the parasitophorous vacuolar membrane (PVM), in the cytoplasm and in the digestive vacuole (DV). Results of extraction and partitioning experiments show that PfCG2 is a peripheral membrane protein. Exposure of trophozoite-infected erythrocytes to trypsin-containing buffer after streptolysin O permeabilization indicates that PfCG2 is exposed to the erythrocyte cytosol at the outer face of the PVM. PfCG2 is highly susceptible to hydrolysis by aspartic and …