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Full-Text Articles in Life Sciences

Behavioural Responses Of Stable Flies To Cattle Manure Slurry Associated Odourants, K. Tangtrakulwanich, T. Albuquerque, Gary Brewer, Fred Baxendale, Ludek Zurek, Daniel Miller, David Taylor, Kristina Friesen, Junwei Zhu Feb 2015

Behavioural Responses Of Stable Flies To Cattle Manure Slurry Associated Odourants, K. Tangtrakulwanich, T. Albuquerque, Gary Brewer, Fred Baxendale, Ludek Zurek, Daniel Miller, David Taylor, Kristina Friesen, Junwei Zhu

David B. Taylor

Stable flies (Stomoxys calcitrans [Diptera: Muscidae] L.) are blood-feeding synanthropic pests, which cause significant economic losses in livestock. Stable fly antennae contain olfactory sensilla responsive to host and host environment-associated odours. Field observation indicated that the abundance of stable flies increased significantly in grasslands or crop fields when cattle manure slurry was applied. Major volatile compounds emanating from manure slurry were collected and identified. Behavioural responses of stable flies to those compounds were investigated in laboratory bioassays and field-trapping studies. Results from olfactometer assays revealed that phenol, p-cresol and m-cresol were attractive to adult stable flies. When tested individually, attraction …


Comparisons Of Antifeedancy And Spatial Repellency Of Three Natural Product Repellents Against Horn Flies, Haematobia Irritans (Diptera:Muscidae), Junwei Zhu, Gary Brewer, David Boxler, Kristina Friesen, David Taylor Dec 2014

Comparisons Of Antifeedancy And Spatial Repellency Of Three Natural Product Repellents Against Horn Flies, Haematobia Irritans (Diptera:Muscidae), Junwei Zhu, Gary Brewer, David Boxler, Kristina Friesen, David Taylor

David B. Taylor

BACKGROUND: Horn flies are among the most important biting fly pests of cattle in the United States. Horn fly management is largely dependent upon pesticides, which ultimately leads to the rapid development of insecticide resistance. Alternative control strategies, including repellents, have shown promising results in reducing fly biting. In the present study, we examined the efficacy and longevity of recently identified natural product repellents against horn flies. RESULTS: Catnip oil, geraniol and C8910 acids reduced horn fly feeding in a laboratory bioassay and also exhibited spatial repellency in the olfactometer. Residual activity was observed for up to 3 days in …


Stable Fly Control In Cattle Winter Feeding Sites With Novaluron, 2013, David B. Taylor, Kristina A. Friesen, Jerry J. Zhu Dec 2013

Stable Fly Control In Cattle Winter Feeding Sites With Novaluron, 2013, David B. Taylor, Kristina A. Friesen, Jerry J. Zhu

David B. Taylor

No abstract provided.


Spatial–Temporal Dynamics Of Stable Fly (Diptera: Muscidae) Trap Catches In Eastern Nebraska, David B. Taylor, Kristina Friesen, Jerry J. Zhu May 2013

Spatial–Temporal Dynamics Of Stable Fly (Diptera: Muscidae) Trap Catches In Eastern Nebraska, David B. Taylor, Kristina Friesen, Jerry J. Zhu

David B. Taylor

Spatial and temporal relationships among catches of adult stable flies, Stomoxys calcitrans (L.), on sticky traps in eastern Nebraska were evaluated. Twenty-five alsynite sticky traps were placed in a 5 by 5 grid with 1.6-km intervals in a mixed agricultural environment from 2003 to 2011. Denser grids of 45-90 traps were implemented for varying lengths of time during the course of the study. More than two million stable flies were collected over 9 yr. Seasonal abundances based upon total collections from the primary grid of 25 traps were bimodal most years with population peaks in June and September or October. …


Efficacy And Longevity Of Newly Developed Catnip Oil Microcapsules Against Stable Fly Oviposition And Larval Growth, J. J. Zhu, Brian Wienhold, J. J. Wehrle, D. Davis, H. Chen, David B. Taylor, Kristina Friesen, L. Zurek Dec 2012

Efficacy And Longevity Of Newly Developed Catnip Oil Microcapsules Against Stable Fly Oviposition And Larval Growth, J. J. Zhu, Brian Wienhold, J. J. Wehrle, D. Davis, H. Chen, David B. Taylor, Kristina Friesen, L. Zurek

David B. Taylor

The stable fly, Stomoxys calcitrans (Diptera: Muscidae), is one of the most important pests of cattle and costs U.S. cattle producers billions of dollars in losses annually. In this study, the efficacy of catnip oil encapsulated in gelatin in oviposition deterrence and larval growth inhibition in stable flies was examined under laboratory conditions. More than 98% inhibition of stable fly larval growth and female oviposition was observed in larval and oviposition media treated with encapsulated catnip oil (0.5 g). Further, dose–response tests showed that as little as 0.1 g of encapsulated catnip oil provided >85% oviposition deterrence. The release of …


Population Genetics And Gene Variation Of Stable Fly Populations (Diptera: Muscidae) In Nebraska, Allen L. Szalanski, David B. Taylor, Richard D. Peterson Ii Feb 2012

Population Genetics And Gene Variation Of Stable Fly Populations (Diptera: Muscidae) In Nebraska, Allen L. Szalanski, David B. Taylor, Richard D. Peterson Ii

David B. Taylor

Genetic variation in stable fly, Stomoxys calcitrans (L.),populations from Nebraska, Canada, and Texas was sampled. Four of 12 allozyme loci were polymorphic, with an average of 1.7 alleles per locus. Observed and expected heterozygosities were 0.086 and 0.070,respectively. Nei's genetic distance between populations averaged 0.001 and ranged from 0.000 to 0.005. Wright's F statistics revealed greater variation within than among populations. Allele frequencies were homogeneous among temporal samples from a single population. Polymerasechain reaction—restriction fragment length polymorphism (PCR—RFLP) analysis of 6.4 kb of the mitochondrial DNA genome with 16 restriction enzymes revealed no variation in stable fly populations from Canada, …


Sugar Feeding In Adult Stable Flies, David Taylor, Dennis R. Berkebile Feb 2012

Sugar Feeding In Adult Stable Flies, David Taylor, Dennis R. Berkebile

David B. Taylor

Adult stable flies (Stomoxys calcitrans L.) are known to feed readily on sugars in the laboratory. However, little is known concerning the extent of stable fly sugar feeding in wild populations. We examined the frequency of sugar feeding in stable flies collected on Alsynite sticky traps in rural and urban environments. In addition, stable flies were visually examined to determine whether blood was present in the gut. In laboratory studies, sugars were detectable with the anthrone technique in stable flies for ~3 d after being imbibed, and blood could be visually detected in the gut for 24-48 h after feeding. …


Economic Impact Of Stable Flies (Diptera: Muscidae) On Cattle Production., David Taylor, Roger Moon, Darrell Mark Dec 2011

Economic Impact Of Stable Flies (Diptera: Muscidae) On Cattle Production., David Taylor, Roger Moon, Darrell Mark

David B. Taylor

Stable flies, Stomoxys calcitrans (L.), are among the most damaging arthropod pests of cattle worldwide. The last estimate of their economic impact on United States cattle production was published 20 yr ago and placed losses at $608 million. Subsequently, several studies of effects of stable flies on beef cattle weight gain and feed efficiency have been published, and stable flies have become increasingly recognized as pests of cattle on pasture and range. We analyzed published studies and developed yield-loss functions to relate stable fly infestation levels to cattle productivity, and then estimated the economic impact of stable flies on cattle …


Substrate Properties Of Stable Fly (Diptera: Muscidae) Developmental Sites Associated With Round Bale Hay Feeding Sites In Eastern Nebraska, Brian J. Wienhold, David B. Taylor Dec 2011

Substrate Properties Of Stable Fly (Diptera: Muscidae) Developmental Sites Associated With Round Bale Hay Feeding Sites In Eastern Nebraska, Brian J. Wienhold, David B. Taylor

David B. Taylor

Residues at sites where stationary feeders were used to provide hay as supplemental forage for cattle during the winter are developmental substrates for immature stable flies, Stomoxys calcitrans (L.), in the central United States. Spatial patterns in physical (substrate depth, temperature, water content), chemical (pH, electrical conductivity [EClab], total nitrogen [N] and carbon [C], ammoniacal nitrogen [NH4-N], extractable phosphorus [P]), and biological (microbial respiration rate) substrate properties for two feeding sites were estimated and the correlations between these properties and adult emergence were characterized. Hay feeding sites had a circular footprint with residues extending ≈7m from …


Dewatered Sewage Biosolids Provide A Productive Larval Habitat For Stable Flies And House Flies (Diptera: Muscidae), Carl W. Doud, David B. Taylor, Ludek Zurek Dec 2011

Dewatered Sewage Biosolids Provide A Productive Larval Habitat For Stable Flies And House Flies (Diptera: Muscidae), Carl W. Doud, David B. Taylor, Ludek Zurek

David B. Taylor

Species diversity and seasonal abundance of muscoid flies (Diptera: Muscidae) developing in biosolid cake (dewatered biosolids) stored at a wastewater treatment facility in northeastern Kansas were evaluated. Emergence traps were deployed 19 May through 20 October 2009 (22 wk) and 27 May through 18 November 2010 (25 wk). In total, 11,349 muscoid flies were collected emerging from the biosolid cake. Stable flies (Stomoxys calcitrans (L.)) and house flies (Musca domestica (L.)), represented 80 and 18% of the muscoid flies, respectively. An estimated 550 stable flies and 220 house flies per square-meter of surface area developed in the biosolid …


Efficacy Of Cyromazine To Control Immature Stable Flies (Diptera: Muscidae) Developing In Winter Hay Feeding Sites, David B. Taylor, Kristina Friesen, Jerry J. Zhu, Kai Sievert Dec 2011

Efficacy Of Cyromazine To Control Immature Stable Flies (Diptera: Muscidae) Developing In Winter Hay Feeding Sites, David B. Taylor, Kristina Friesen, Jerry J. Zhu, Kai Sievert

David B. Taylor

Hay mixed with manure and urine residues at sites where hay has been provided as supplemental winter feed for cattle provide an excellent substrate for the development of immature stable flies, Stomoxys calcitrans (L.). Such sites are primary sources of early summer stable flies in the central United States and no effective measures are currently available to control fly development in them. A single application of granular cyromazine in May provided 97% reduction in the number of adult stable flies emerging from hay feeding sites. Stable fly control did not decline during the 12 wk season. A small decline in …


Response Of Growing Calves To Stable Flies., Lisa A. Schole, David B. Taylor, Dennis R. Brink Dec 2010

Response Of Growing Calves To Stable Flies., Lisa A. Schole, David B. Taylor, Dennis R. Brink

David B. Taylor

No abstract provided.


Phenology Of Stable Fly (Diptera: Muscidae) Larvae In Round Bale Hay Feeding Sites In Eastern Nebraska., David B. Taylor, Dennis R. Berkebile Dec 2010

Phenology Of Stable Fly (Diptera: Muscidae) Larvae In Round Bale Hay Feeding Sites In Eastern Nebraska., David B. Taylor, Dennis R. Berkebile

David B. Taylor

The temporal and spatial patterns of adult stable fly, Stomoxys calcitrans (L.), emergence from six sites where large round baled hay had been provided to pasture cattle as winter feed were studied using emergence traps. The substrate at these sites, consisting of waste hay mixed with bovine manure and urine, provided an excellent developmental habitat for immature stable flies. Stable flies were the most frequently collected fly emerging from these sites with a yearly average of 1,581 emerging per square meter. Stable fly emergence from these sites began in early May (235 annual accumulated Day-Degree 10°C [DD10]), peaked …


Dispersal Of Stable Flies (Diptera: Muscidae) From Larval Developmental Sites., David B. Taylor, Roger D. Moon, John B. Campbell, Dennis R. Berkebile, Philip J. Scholl, Alberto B. Broce, Jerome A. Hogsette Dec 2009

Dispersal Of Stable Flies (Diptera: Muscidae) From Larval Developmental Sites., David B. Taylor, Roger D. Moon, John B. Campbell, Dennis R. Berkebile, Philip J. Scholl, Alberto B. Broce, Jerome A. Hogsette

David B. Taylor

Seven mark-recapture studies were conducted over 3 yr to assess dispersal of newly emerging adult stable flies, Stomoxys calcitrans L., from larval development sites in a mixed agricultural environment in northeastern Nebraska. Infested hay debris piles were marked by dusting their surfaces with fluorescent pigments, adults were captured with surrounding grids of Alsynite sticky traps, and specimens were dissected to determine feeding histories and reproductive age. Distances and directions of 3,889 marked specimens indicated males and females dispersed equally and in all directions. Midguts of males and females were equally likely to contain blood-meal remnants. Percentage with blood remnants and …


A New Method For Collecting Clean Stable Fly (Diptera: Muscidae) Pupae Of Known Age., Dennis Berkebile, David Taylor, Anthony Weinhold Dec 2008

A New Method For Collecting Clean Stable Fly (Diptera: Muscidae) Pupae Of Known Age., Dennis Berkebile, David Taylor, Anthony Weinhold

David B. Taylor

Stable flies, Stomoxys calcitrans L., are important pests of confined and pasture cattle. They have been reared in the laboratory to study their biology and to test new methods of control. Research on rearing modifications has concentrated on developing larval diets from materials locally abundant. Under current protocols, pupae form in the medium. Aggregations of pupae were located and removed, often with a considerable amount of extraneous material. Various methods have been developed to separate the pupae from waste material. We describe a method by which wandering larvae are enticed to leave the medium prior to pupariation. The larvae were …


Stable Fly Population Dynamics In Eastern Nebraska In Relation To Climatic Variables., David B. Taylor, Dennis R. Berkebile, Philip J. Scholl Dec 2006

Stable Fly Population Dynamics In Eastern Nebraska In Relation To Climatic Variables., David B. Taylor, Dennis R. Berkebile, Philip J. Scholl

David B. Taylor

Stable flies, Stomoxys calcitrans (L.), are among the most economically important arthropod pests of livestock in North America. In this study, we monitored the seasonal dynamics of a stable fly population in eastern Nebraska for 5 yr. Models based upon temperature and precipitation were developed to determine the affects of these variables on population levels as well as to project population trends. Stable flies appear in eastern Nebraska in late March to early April, and they build to a peak population during the last week of June and first week of July. In most years, the population decreases in midsummer, …


Comparative Efficiency Of Six Stable Fly Traps., David B. Taylor, Dennis R. Berkebile Dec 2005

Comparative Efficiency Of Six Stable Fly Traps., David B. Taylor, Dennis R. Berkebile

David B. Taylor

Five adhesive traps and the Nzi cloth-target trap were compared to determine their trapping efficiency and biases for stable flies, Stomoxys calcitrans (L.) (Diptera: Muscidae). Two configurations of the BiteFree prototype trap, constructed of polyethylene terephthalate, were most efficient for trapping stable flies, whereas the EZ trap was least efficient. The two Alsynite traps, Broce and Olson, were intermediate to the BiteFree prototype and EZ traps. All adhesive traps collected a ratio of approximately two males for each female. Approximately 50% of the flies collected on the adhesive traps, both male and female, were blood fed, and 20% were vitellogenic. …


Genetics Of The Stable Fly., David B. Taylor Dec 1994

Genetics Of The Stable Fly., David B. Taylor

David B. Taylor

No abstract provided.