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Assessing Nuclear And Mitochondrial Dna Sequence Variation Within Steinernema (Rhabditida: Steinernematidae), Allen L. Szalanski, David B. Taylor, Peter G. Mullin
Assessing Nuclear And Mitochondrial Dna Sequence Variation Within Steinernema (Rhabditida: Steinernematidae), Allen L. Szalanski, David B. Taylor, Peter G. Mullin
David B. Taylor
DNA sequence analysis was used to characterize the nuclear ribosomal DNA ITS1 region and a portion of the COII and 16S rDNA genes of the mitochondrial genome from Steinernema entomopathogenic nematodes. Nuclear ITS1 nucleotide divergence among seven Steinernema spp. ranged from 6 to 22%, and mtDNA divergence among five species ranged from 12 to 20%. No intraspecific variation was observed among three S. feltiae strains. Phylogenetic analysis of both nuclear and mitochondrial DNA sequences confirms the existing morphological relationships of several Steinernema species. Both the rDNA ITS1 and mtDNA sequences were useful for resolving relationships among Steinernema taxa.
Susceptibility Of House Fly (Diptera: Muscidae) Larvae To Entomopathogenic Nematodes (Rhabditida: Heterorhabditidae, Steinernematidae, David B. Taylor, Allen L. Szalanski, Byron J. Adams, Richard D. Peterson Ii
Susceptibility Of House Fly (Diptera: Muscidae) Larvae To Entomopathogenic Nematodes (Rhabditida: Heterorhabditidae, Steinernematidae, David B. Taylor, Allen L. Szalanski, Byron J. Adams, Richard D. Peterson Ii
David B. Taylor
The potential for entomopathogenic nematodes to control flies in cattle feedlots was determined by screening 40 strains representing 8 species of Heterorhabditis Poinar and 5 species of Steinernema Travassos for virulence toward 3rd-instar house flies (maggots), Musca domestica L. None of the 22 strains of Heterorhabditis infecting maggots caused significant levels of mortality in a filter paper assay. Ten strains of Steinernema infected maggots, of which 7 strains--4 S. carpocapsa (Weiser), 2 S. feltiae (Filipjev), and 1 S. scapterisci (Nguyen & Smart) caused significant mortality. Ten Heterorhabditis strains and 10 Steinernema strains successllly reproduced for 22 generations in maggots. No …
Virulence And Persistance Of Steinernema Feltiae Towards House Fly, Musca Domestica, In Bovine Manure., Brian P. Clark, David B. Taylor, Allen L. Szalanski, Tomas O. Powers
Virulence And Persistance Of Steinernema Feltiae Towards House Fly, Musca Domestica, In Bovine Manure., Brian P. Clark, David B. Taylor, Allen L. Szalanski, Tomas O. Powers
David B. Taylor
The virulence of Steinernema feltiae strain SN towards house fly, Musca domestica, and nematode persistence in the bovine manure environment was examined. Nematodes were applied at the 95% LD rate of 1x106 nematodes per m2 to thirty two one liter containers containing 150 g of manure. Another thirty two containers without nematodes was the control. At weekly intervals, 100 third instar M. domestica larvae were added to four of the treated and four of the control containers. Survival was determined by adult house fly emergence. There was a twenty percent weekly decline in the infectivity of the …