Life Sciences Commons^™

Fgf2-Induced Effects On Transcriptome Associated With Regeneration Competence In Adult Human Fibroblasts, Olga Kashpur, David Lapointe, Sakthikumar Ambady, Elizabeth Ryder, Tanja Dominko

Sakthikumar Ambady

BACKGROUND: Adult human fibroblasts grown in low oxygen and with FGF2 supplementation have the capacity to tip the healing outcome of skeletal muscle injury - by favoring regeneration response in vivo over scar formation. Here, we compare the transcriptomes of control adult human dermal fibroblasts and induced regeneration-competent (iRC) fibroblasts to identify transcriptional changes that may be related to their regeneration competence. RESULTS: We identified a unique gene-expression profile that characterizes FGF2-induced iRC fibroblast phenotype. Significantly differentially expressed genes due to FGF2 treatment were identified and analyzed to determine overrepresented Gene Ontology terms. Genes belonging to extracellular matrix components, adhesion …

Task-Specific And General Cognitive Effects In Chiari Malformation Type I, Philip Allen, James Houston, Joshua Pollock, Christopher Buzzelli, Xuan Li, A. Harrington, Bryn Martin, Francis Loth, Mei-Ching Lien, Jahangir Maleki, Mark Luciano

Philip A Allen

Our objective was to use episodic memory and executive function tests to determine whether or not Chiari Malformation Type I (CM) patients experience cognitive dysfunction.

Biocompatible Flavone-Based Fluorogenic Probes For Quick Wash-Free Mitochondria! Imaging In Living Cells, Bin Liu, Mickey Shah, Ge Zhang, Qin Liu, Yi Pang

Yi Pang

Mitochondria, vital organelles existing in almost all eukaryotic cells, play a crucial role in energy metabolism and apoptosis of aerobic organisms. In this work, we report two new flavone-based fluorescent probes, MC-Mito1 and MC-Mito2, for monitoring mitochondria in living cells. These two probes exhibit remarkably low toxicity, good cell permeability, and high specificity; these probes complement the existing library of mitochondrial imaging agents. The new dyes give nearly no background fluorescence, and their application does not require tedious postwashing after cell staining. The appreciable tolerance of MC-Mito2 encourages a broader range of biological applications for understanding the cell degeneration and …

Biocompatible Flavone-Based Fluorogenic Probes For Quick Wash-Free Mitochondria! Imaging In Living Cells, Bin Liu, Mickey Shah, Ge Zhang, Qin Liu, Yi Pang

Ge Zhang

Mitochondria, vital organelles existing in almost all eukaryotic cells, play a crucial role in energy metabolism and apoptosis of aerobic organisms. In this work, we report two new flavone-based fluorescent probes, MC-Mito1 and MC-Mito2, for monitoring mitochondria in living cells. These two probes exhibit remarkably low toxicity, good cell permeability, and high specificity; these probes complement the existing library of mitochondrial imaging agents. The new dyes give nearly no background fluorescence, and their application does not require tedious postwashing after cell staining. The appreciable tolerance of MC-Mito2 encourages a broader range of biological applications for understanding the cell degeneration and …

Magnetic Labeling Of Bm-Msc-Derived Smcs Maintains Their Pro-Elastogenic Trophic Effects On Aneurysmal Smcs, G. Swaminathan, B. Sivaraman, I. Stoilov, Mickey Shah, Ge Zhang, R.P. Mecham, A. Ramamurthi

Ge Zhang

No abstract provided.

Three-Dimensional Confocal Microscopy Indentation Method For Hydrogel Elasticity Measurement, Donghee Lee, Md Mahmudur Rahman, You Zhou, Sangjin Ryu

Md Mahmudur Rahman

No abstract provided.

Hemo: A Sustainable Multi-Objective Evolutionary Optimization Framework, Jianjun Hu, K. Seo, Z. Fan, R. Rosenberg, E. Goodman

Jianjun Hu

No abstract provided.

Improving Protein Localization Prediction Using Amino Acid Group Based Physichemical Encoding, Jianjun Hu, F. Zhang

Jianjun Hu

No abstract provided.

Scored Protein-Protein Interaction To Predict Subcellular Localizations For Yeast Using Diffusion Kernel, A. Mondal, Jianjun Hu

Jianjun Hu

No abstract provided.

Robust And Efficient Genetic Algorithms With Hierarchical Niching And A Sustainable Evolutionary Computation Model, Jianjun Hu, E. Goodman

Jianjun Hu

No abstract provided.

A Novel Mutation Of Cyld In A Chinese Family With Multiple Familial Trichoepithelioma, Z. Ying, H. Ma, Y. Liu, S. Xiao, Y. Wang, Guo-Xiang Wang

Dr. Guo-Xiang Wang

Trichoepithelioma is a benign cutaneous tumour that originates from hair follicles and occurs either as a sporadic non-familial or a multiple-familial type. Recently, several mutations in the cylindromatosis (CYLD) gene have been reported in multiple familial trichoepithelioma (MFT).

Task-Specific And General Cognitive Effects In Chiari Malformation Type I, Philip Allen, James Houston, Joshua Pollock, Christopher Buzzelli, Xuan Li, A. Harrington, Bryn Martin, Francis Loth, Mei-Ching Lien, Jahangir Maleki, Mark Luciano

Dr. Bryn Martin

Our objective was to use episodic memory and executive function tests to determine whether or not Chiari Malformation Type I (CM) patients experience cognitive dysfunction.

Reduction Of Water Surface Tension Significantly Impacts Gecko Adhesion Underwater, Peter Niewiarowski, Ali Dhinojwala, Alyssa Stark, Brandon Mcclung

Dr. Peter H. Niewiarowski

The gecko adhesive system is dependent on weak van der Waals interactions that are multiplied across thousands of fine hair-like structures (setae) on geckos' toe pads. Due to the requirements of van der Waals forces, we expect that any interruption between the setae and substrate, such as a water layer, will compromise adhesion. Our recent results suggest, however, that the air layer (plastron) surrounding the superhydrophobic toe pads aid in expelling water at the contact interface and create strong shear adhesion in water when in contact with hydrophobic surfaces. To test the function of the air plastron, we reduced the …

The Role Of Surface Chemistry In Adhesion And Wetting Of Gecko Toe Pads, Ali Dhinojwala, Peter Niewiarowski, Ila Badge, Alyssa Stark, Eva Paoloni

Dr. Peter H. Niewiarowski

An array of micron-sized setal hairs offers geckos a unique ability to walk on vertical surfaces using van der Waals interactions. Although many studies have focused on the role of surface morphology of the hairs, very little is known about the role of surface chemistry on wetting and adhesion. We expect that both surface chemistry and morphology are important, not only to achieve optimum dry adhesion but also for increased efficiency in self-cleaning of water and adhesion under wet conditions. Here, we used a plasma-based vapor deposition process to coat the hairy patterns on gecko toe pad sheds with polar …

Books And Our Human Stories, Paul Benson

Paul H. Benson

An essay on the impact of the works in the Imprints and Impressions: Milestones in Human Progress, an exhibition of rare books from the collection of Stuart Rose. Exhibition was held Sept. 29-Nov. 9, 2014, at the University of Dayton.

Effect Of Topology On The Adhesive Forces Between Electrospun Polymer Fibers Using A T-Peel Test, Florencia Ballarin, Todd Blackledge, Nicole Capitos Davis, Partrica Frontini, Gustavo Abraham, Shing-Chung Wong

Todd A. Blackledge

Electrospinning provides an effective methodology to obtain high aspect ratio polymer fibers for biomimetic applications. In this article, we evaluate the effect of topology on adhesion between aligned fibers. Polycaprolactone is electrospun using two different setups: (i) a tip collector and (ii) a flat collector. The tip collector enables the fibers to self-align. When a fiber reaches the tip collector, the next fiber is repelled by the charge they carry, forcing the fibers to deposit in a parallel arrangement. The flat collector allows the fibers to deposit at random. The adhesion between the fiber mats is measured using a T-peel …

Educational Data Mining: An Advance For Intelligent Systems In Education, Ryan Baker

Ryan S.J.d. Baker

Computer-based technologies have transformed the way we live, work, socialize, play, and learn. Today, the use of data collected through these technologies is supporting a second-round of transformation in all of these areas. Over the last decades, the methods of data mining and analytics have transformed field after field. Scientific fields such as physics, biology, and climate science have leveraged these methods to manage and make discoveries in previously unimaginably large datasets. The first journal devoted to data mining and analytics methods in biology, Computers in Biology and Medicine, began publication as long ago as the 1970s. In the mid-1990s …

Open Access To Stm Information: Trends, Models, And Strategies For Libraries (Review), Steven Van Tuyl

Steven I Van Tuyl

No abstract provided.

Proteomic Analysis Of 17Β-Estradiol Degradation By Stenotrophomonas Maltophilia, Zhongtian Li

Z Li

Microbial degradation plays a critical role in determining the environmental fate of steroid hormones, such as 17β-estradiol (E2). The molecular mechanisms governing the microbial transformation of E2 and its primary degradation intermediate, estrone (E1), are largely unknown. The objective of this study was to identify metabolism pathways that might be involved in microbial estrogen degradation. To achieve the objective, Stenotrophomonas maltophilia strain ZL1 was used as a model estrogen degrading bacterium and its protein expression level during E2/E1 degradation was studied using quantitative proteomics. During an E2 degradation experiment, strain ZL1 first converted E2 to E1 stoichiometrically. At 16 h …

Self-Assembling Short Oligopeptides And The Promotion Of Angiogenesis, Alisha Sarang-Sieminski, Daria Narmoneva, Olumuyiwa Oni, Shugang Zhang, Jonathan Gertler, Roger Kamm, Richard Lee

Alisha L. Sarang-Sieminski

Because an adequate blood supply to and within tissues is an essential factor for successful tissue regeneration, promoting a functional microvasculature is a crucial factor for biomaterials. In this study, we demonstrate that short self-assembling peptides form scaffolds that provide an angiogenic environment promoting long-term cell survival and capillary-like network formation in three-dimensional cultures of human microvascular endothelial cells. Our data show that, in contrast to collagen type I, the peptide scaffold inhibits endothelial cell apoptosis in the absence of added angiogenic factors, accompanied by enhanced gene expression of the angiogenic factor VEGF. In addition, our results suggest that the …

Salmon Fibrin Supports An Increased Number Of Sprouts And Decreased Degradation While Maintaining Sprout Length Relative To Human Fibrin In An In Vitro Angiogenesis Model, Alisha Sarang-Sieminski, Keith Gooch

Alisha L. Sarang-Sieminski

Salmon-derived fibrin has been proposed as a preferred alternative to human or bovine fibrin because of its reduced potential for disease transmission. Here we evaluate salmon fibrin as an alternative ECM support for therapeutic angiogenesis applications, such as vascularizing engineered tissues. Human umbilical vein endothelial cells (HUVEC) seeded on gelatin beads and suspended in either salmon or human fibrin sprouted and formed capillary-like structures. Sprout length was generally increased with the addition of bFGF and VEGF and further increased with the addition of phorbol myristate acetate (PMA). The number of sprouts per bead was increased 61-188% in salmon fibrin relative …

Removing 17Β-Estradiol From Drinking Water In A Biologically Active Carbon (Bac) Reactor Modified From A Granular Activated Carbon (Gac) Reactor, Zhongtian Li

Z Li

Estrogenic compounds in drinking water sources pose potential threats to human health. Treatment technologies are needed to effectively remove these compounds for the production of safe drinking water. In this study, GAC adsorption was first tested for its ability to remove a model estrogenic compound, 17β-estradiol (E2). Although GAC showed a relatively high adsorption capacity for E2 in isotherm experiments, it appeared to have a long mass transfer zone in a GAC column reactor, causing an early leakage of E2 in the effluent. With an influent E2 concentration of 20 μg/L, the GAC reactor was able to bring down effluent …

Forisome Performance In Artificial Sieve Tubes, Michael Knoblauch, Mike Stubenrauch, Aart J.E. Van Bel, Winfried S. Peters

Winfried S. Peters

2011 Scholars And Artists Bibliography, Mark Tebeau

Mark Tebeau

This bibliography was created for the annual Friends of the Michael Schwartz Library Scholars and Artists Reception, recognizing scholarly and creative achievements of Cleveland State University faculty, staff and emeriti. Mark Tebeau was the guest speaker

Toxicity Of Cdse Nanoparticles In Caco-2 Cell Cultures, Lin Wang, Dattatri Nagesha, Selvapraba Selvarasah, Mehmet Dokmeci, Rebecca Carrier

Mehmet R. Dokmeci

Background
Potential routes of nanomaterial exposure include inhalation, dermal contact, and ingestion. Toxicology of inhalation of ultra-fine particles has been extensively studied; however, risks of nanomaterial exposure via ingestion are currently almost unknown. Using enterocyte-like Caco-2 cells as a small intestine epithelial model, the possible toxicity of CdSe quantum dot (QD) exposure via ingestion was investigated. Effect of simulated gastric fluid treatment on CdSe QD cytotoxicity was also studied.

Results
Commercially available CdSe QDs, which have a ZnS shell and poly-ethylene glycol (PEG) coating, and in-house prepared surfactant coated CdSe QDs were dosed to Caco-2 cells. Cell viability and attachment …

Münch, Morphology, Microfluidics – Our Structural Problem With The Phloem [Review Article], Michael Knoblauch, Winfried S. Peters

Winfried S. Peters

Anisotropic Contraction In Forisomes: Simple Models Won't Fit, Winfried Peters, Michael Knoblauch, Stephen Warmann, William Pickard, Amy Shen

Winfried S. Peters

Tailed Forisomes Of Canavalia Gladiata: A New Model To Study Ca2+-Driven Protein Contractility, Winfried Peters, Michael Knoblauch, Stephen Warmann, Reinhard Schnetter, Amy Shen, William Pickard

Winfried S. Peters

The Geometry Of The Forisome–Sieve Element–Sieve Plate Complex In The Phloem Of Vicia Faba L. Leaflets, Winfried Peters, Aart Van Bel, Michael Knoblauch

Winfried S. Peters

Forisome Based Biomimetic Smart Materials, Amy Shen, Benjamin Hamlington, Michael Knoblauch, Winfried Peters, William Pickard

Winfried S. Peters