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Defining Gene Expression In Normal Human Epidermal Keratinocytes And Melanocytes: A Prerequisite For Understanding Hyperplastic And Neoplastic Pathology, Erika De Wyllie Billick
Defining Gene Expression In Normal Human Epidermal Keratinocytes And Melanocytes: A Prerequisite For Understanding Hyperplastic And Neoplastic Pathology, Erika De Wyllie Billick
Student Theses and Dissertations
To define pathologic alterations, a reference of "normal" cells is needed in order to interpret genomic methods that study gene expression of melanocytes and keratinocytes in growth-activated or neoplastic skin diseases. Historically, mRNAs isolated from cultured epidermal keratinocytes or melanocytes are used to define normal gene expression patterns. In this study, we profiled global gene expression in human epidermal keratinocytes on Affymetrix U133 plus 2.0 arrays from three different "normal" sources: 1) cultured keratinocytes, 2) FACS keratinocytes from dispase-separated epidermis, and 3) laser-capture microdissected (LCM) epidermis. For melanocytes, the precursor cell of melanoma, the attempt was made to isolate a …