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Full-Text Articles in Life Sciences
Silencing Mist1 Gene Expression Is Essential For Recovery From Acute Pancreatitis, Anju Karki, Sean E. Humphrey, Rebecca E. Steele, David A. Hess, Elizabeth J. Taparowsky, Stephen F. Konieczny
Silencing Mist1 Gene Expression Is Essential For Recovery From Acute Pancreatitis, Anju Karki, Sean E. Humphrey, Rebecca E. Steele, David A. Hess, Elizabeth J. Taparowsky, Stephen F. Konieczny
Department of Biological Sciences Faculty Publications
Acinar cells of the exocrine pancreas are tasked with synthesizing, packaging and secreting vast quantities of pro-digestive enzymes to maintain proper metabolic homeostasis for the organism. Because the synthesis of high levels of hydrolases is potentially dangerous, the pancreas is prone to acute pancreatitis (AP), a disease that targets acinar cells, leading to acinar-ductal metaplasia (ADM), inflammation and fibrosis-events that can transition into the earliest stages of pancreatic ductal adenocarcinoma. Despite a wealth of information concerning the broad phenotype associated with pancreatitis, little is understood regarding specific transcriptional regulatory networks that are susceptible to AP and the role these networks …
On The Origin Of Protein Superfamilies And Superfolds, Abram Magner, Wojciech Szpankowski, Daisuke Kihara
On The Origin Of Protein Superfamilies And Superfolds, Abram Magner, Wojciech Szpankowski, Daisuke Kihara
Department of Biological Sciences Faculty Publications
Distributions of protein families and folds in genomes are highly skewed, having a small number of prevalent superfamiles/superfolds and a large number of families/folds of a small size. Why are the distributions of protein families and folds skewed? Why are there only a limited number of protein families? Here, we employ an information theoretic approach to investigate the protein sequence-structure relationship that leads to the skewed distributions. We consider that protein sequences and folds constitute an information theoretic channel and computed the most efficient distribution of sequences that code all protein folds. The identified distributions of sequences and folds are …
Expression And Misexpression Of The Mir-183 Family In The Developing Hearing Organ Of The Chicken, Kaidi D. Zhang, Michelle L. Stoller, Donna M. Fekete
Expression And Misexpression Of The Mir-183 Family In The Developing Hearing Organ Of The Chicken, Kaidi D. Zhang, Michelle L. Stoller, Donna M. Fekete
Department of Biological Sciences Faculty Publications
The miR-183 family consists of 3 related microRNAs (miR-183, miR-96, miR-182) that are required to complete maturation of primary sensory cells in the mammalian inner ear. Because the level of these microRNAs is not uniform across hair cell subtypes in the murine cochlea, the question arises as to whether hair cell phenotypes are influenced by microRNA expression levels. To address this, we used the chicken embryo to study expression and misexpression of this gene family. By in situ hybridization, expression of all 3 microRNAs is robust in immature hair cells of both auditory and vestibular organs and is present in …
Rna-Guided Crispr-Cas Technologies For Genome-Scale Investigation Of Disease Processes, Sean E. Humphrey, Andrea L. Kasinski
Rna-Guided Crispr-Cas Technologies For Genome-Scale Investigation Of Disease Processes, Sean E. Humphrey, Andrea L. Kasinski
Department of Biological Sciences Faculty Publications
From its discovery as an adaptive bacterial and archaea immune system, the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas system has quickly been developed into a powerful and groundbreaking programmable nuclease technology for the global and precise editing of the genome in cells. This system allows for comprehensive unbiased functional studies and is already advancing the field by revealing genes that have previously unknown roles in disease processes. In this review, we examine and compare recently developed CRISPR-Cas platforms for global genome editing and examine the advancements these platforms have made in guide RNA design, guide RNA/Cas9 interaction, on-target specificity, …