Life Sciences Commons^™

Lack Of Replication Disruption Following H2o2-Induced Damage In Escherichia Coli, Sierra S. Schmidt, Justin Courcelle, Charmain T. Courcelle

University Honors Theses

Hydrogen peroxide (H₂O₂) toxicity has long been thought to be predominantly due to oxidative DNA damage that can disrupt DNA replication and result in lethality. Curiously and contrary to this view, it is also well established that the glycosylases responsible for repairing oxidized-base damage are as resistant as wild-type cells when treated with H₂O₂. The observation raises the possibility that H₂O₂-induced DNA damage does not disrupt or prevent replication. To examine this possibility, I examined the sensitivity of recF mutants to H₂O₂. RecF is …