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The Dna Sequence Required For The Maximal Transactivation Of The Vp5 Gene Of Herpes Simplex Virus Type 1, Shin Chen
Dissertations and Theses
A regulatory element involved in the transcriptional activation of the major capsid protein (VP5) of herpes simplex virus type 1 was identified and characterized in this research project. Gel mobility shift assay with nuclear extracts from both uninfected and HSV-1 infected HeLa cells identified two major protein-DNA complexes involving the VP5 promoter. No viral specific complex found. DNase I and orthophenanthroline-Cu+ footprint analyses in the same laboratory revealed that the two complexes involve a single binding site, GGCCATCTTGAA, located between -64 and -75 bp relative to the VP5 cap site. To determine the function of this leaky-late binding site …
Studies On The Role Of Cellular Factor, Yy1, In Herpes Simplex Virus Type 1 Late Gene Expression, Xuehui Liu
Studies On The Role Of Cellular Factor, Yy1, In Herpes Simplex Virus Type 1 Late Gene Expression, Xuehui Liu
Dissertations and Theses
The herpes simplex virus 1 (HSVl) VP5 gene codes for the major viral capsid protein. Understanding of the mechanism of how the VP5 gene is regulated in host cells will help us to understand the molecular action of the HSV 1 life cycle and its interplay with the host cell gene expression machinery (transcription and translation). This may ultimately provide scientific bases for both better prevention and cure of HSV 1 caused diseases. Previous work from Dr. Robert L. Millette' s laboratory has indicated that a 164 base pair region of the VP5 promoter gene could activate the transcription of …