Life Sciences Commons^™

Genetic Transformation Among Azotobacter Species, Wayne H. Voth

Dissertations and Theses

Previous methods for genetic transformation in Azotobacter vinelandii have employed poorly defined genetic markers or crude DNA extracts. An improved transformation technique has been developed for use in Azotobacter. The technique was used to transform several strains of Azotobacter with DNA carrying a defined genetic marker. A method for isolating pure, high molecular weight, biologically active DNA from Azotobacter is also presented. Purity of the extracted DNA was determined by standard chemical assays. The molecular weight was determined by boundary sedimentation techniques to be 18.2 megadaltons. DNA was obtained from several mutant strains of Azotobacter. Biological activity of these samples …

Bird Use Of Revetted Riverbanks In The Willamette Valley, Clifford Brian Perry

Dissertations and Theses

Over 115 miles of rock revetments that serve to protect river banks have been constructed in the Willamette Basin. In this study the spring and summer bird use of Willamette Basin revetments is examined. Revetments that had not been recently cleared of most woody vegetation and blackberries were found to have significantly greater total and breeding bird use than cleared revetments. Possible factors affecting bird use, such as vegetation on revetments and vegetation adjacent to revetments are examined using linear regression analysis. The results of this study are compared with results of previous work in riparian forests along the Columbia …

Distribution And Behavior Study Of Diurnal Tree Squirrels In Portland, Oregon, With Emphasis On The Western Gray Squirrel (Sciurus Griseus Griseus Ord) And The Western Fox Squirrel (S. Niger Rufiventer E. Geoffroy St.-Hilaire), Ira Young Rice Iii

Dissertations and Theses

Populations of diurnal tree squirrels in the Portland area were located by mail surveys, personal interviews, and field inspections. Pine squirrels were found to reside in thickly wooded residential areas with dense understories and running water nearby. Western gray squirrels occupied habitats in relatively quiet residential neighborhoods, in areas well supplied with mast crops. Western fox squirrels were found to coexist with gray squirrels in their preferred habitat, and also to thrive in park settings with few mast trees and high activity and noise levels. In areas occupied by both fox and gray squirrels, dominant-subordinate relationships were noted. Each species …

Effects Of Types 1 And 2 Herpes Simplex Viruses On Several Fish Tissue Culture Systems, Raymond Charles Harry

Dissertations and Theses

The purpose of this investigation was to observe changes induced by types 1 and 2 herpes simplex viruses (HSV 1 and HSV 2) when these viruses were cultivated in fish cells. The possibility of attempting to use human strains of herpesviruses in order to transform normal fish tissue culture preparations was considered. Three different fish cell cultures were tested: CHSE-214 cell lines were derived from embryonic chinook salmon (Oncorhynchus tshawytscha), and STE-137 cell lines were obtained from embryonic steelhead trout (Salmo gairdneri). Cells isolated from immature ovaries of rainbow trout (Salmo gairdneri) were propagated …

Bat Homing, John Mark Perkins

Dissertations and Theses

A model proposed by Wilson & Findley (1972) to test for randomness in bat homing was applied to results of homing studies on big brown bats (Eptesticus fuscus) and, when possible, to published studies on homing by other bats.

Most applications of the model suggest randomness in bat homing. Ecological notes concerning parturition, parasites, populations, emergence time, flight behavior and morphology of the E. Fuescus colony are reported.

A Behavioral Study Of A Small Group Of Pikas (Ochotona Princeps) On Mt. Hood, Oregon, Nancy A. Ewen Frazier

Dissertations and Theses

Observations or a small group of pikas, (Ochotona princeps) occupying a southwest facing roadfill on Mt. Hood, Oregon (1518 m) were made during the fall or 1976. Territorial sizes of 305.5 m² and 121 m² were determined based on territorial displays. These territories were compared with those observed in other studies. The mean distance to the nearest neighbor was 15.1 m. A pair relationship between two of the pikas and a dominance order are discussed. The seasonal progression of the haypiles and the reactions of the pikas to other animals are also noted.

Behavioral Temperature Regulation In The Turtle Pseudemys Scripta Elegans, Michael H. Johnston

Dissertations and Theses

A group of red-eared turtles (Pseudemys scripta elegans) was acclimated to 18-20˚C and artificial photo-period for 21 days. The animals were then individually placed in a thermal gradient and their selected temperatures were continuously recorded for 24 hours. A second group of turtles was acclimatized to 7.5-11.0˚C and natural photoperiods for 28 days, and subsequently tested in the same manner as the first group. Lastly, the turtles in the first group, after being acclimated to 3.0˚C and the previous artificial photoperiod for 14 days, were retested in the gradient.

The Fatty Acid Composition Of Some North American Dwarf Mistletoe, Hwa Chen

Dissertations and Theses

Fatty acid composition of five dwarf mistletoe species was analysed by Gas Liquid Chromatography. Because of the limitations of available equipment, only aerial shoots were analysed. Palmitic, oleic, linoleic and arachidic acids were the major components of total lipids. The sum of these acids accounted for 85-95 percent of total lipids present in the tissue. There was no apparent difference between species. The ratio of saturated acids to unsaturated acids in summer collections was higher than that of fall collections. Temperature may play an important role in controlling of these ratios. Lipid content varied from 3.25% to 8.5% on a …

A Study Of Home Range In Two Neotoma Fuscipes Colonies In Klamath County, Oregon, Brent D. Frazier

Dissertations and Theses

Home ranges of dusky-footed wood rats (Neotoma fuscipes) were determined by the exclusive boundary strip method in two colonies 630 m apart in Klamath County, Oregon. The mean home range area was 1800 - 1900 m² . Densities or trees and shrubs were determined on selected home ranges and densities of wood rat houses and nests were determined on all home ranges. No significant correlation was found between home range areas and densities of various trees and shrubs, home range areas and densities of woodrat houses and nests, or home range areas and weights of woodrats in …

The Effect Of Declining Timber Supplies And Productivity Increases In The Forest Products Industries Upon Employment In Douglas County, Oregon, Patrick L. Burden

Dissertations and Theses

This thesis examines some of the factors that will have significant impact upon employment in Douglas County, Oregon to the year 2000.

The major question this research attempts to answer is: Given a continuation of current policies and programs, what will future employment levels in the forest product industries and total employment levels in all industries in Douglas County be to the year 2000.

Localization Of Neurosecretory Cells Within The Cerebral Ganglia Of Amphiphorus Imparispinosus Griffin, 1898 (Hoplonemertea) And Their Possible Regulatory Role In The Annual Reproductive Cycle, Gail H. Jacobson

Dissertations and Theses

Nemerteans are common invertebrates along coastlines in both temperate and arctic climates throughout the world. Amphiphorus imparispinosus is a carnivorous member of this phylum that is found abundantly in the intertidal areas of the Oregon coast. As in many other invertebrate phyla, endocrine integration within the nemerteans is thought to be accomplished by neurosecretory mechanisms. In Amphiphorus the presence of these neurosecretory systems has not been previously shown. This project was undertaken to determine if neurosecretory cells are present within the central nervous system of this species and. further, to examine seasonal changes in the number and appearance of these …

The Dispersal And Dispersion Patterns Of Hydra Fusca In A Limited Environment, Faith E. Ruffing

Dissertations and Theses

The dispersal and dispersion patterns of Hydra fusca were examined. Hydra were placed in petri dishes at various densities. The water in the dishes was swirled forcing the animals to the center. The location of each animal was marked at time intervals thereafter. Analyses of the dispersal rates and dispersion patterns were made. Hydra dispersed from a central release point at a non-random rate. There was rapid movement from the center followed by a minimal daily movement. This eventually resulted in a uniform dispersion pattern at high densities in a limited environment. There was a relationship between the ratio of …

Purification And Properties Of Lysozyme From Pseudomonas Aeruginosa Bacteriophage 7v, Lynne Vernice Mcfarland

Dissertations and Theses

A lysozyme from Bacteriophage 7v was purified 7.7 fold over the original lysates of the bacteriophage 7v and Pseudomonas aeruginosa PS-7. This purification process includes ultracentrifugation, ammonium sulfate precipitation, dialysis, and fractionation in a Sephadex G-150 column. The phage lysozyme exhibits a greater specificity when assayed with P. aeruginosa cells as a substrate, but still is capable of acting on the standard lysozyme Micrococcus lysodeikticus substrate. The pH optimum, heat inactivation range, and action on other bacteria is described. The molecular weight was found to be 14,300. The values of this 7v phage lysozyme are in close agreement with values …