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Are Semen Quality Parameters Sufficient For Biomonitoring Spermatozoa Dna Integrity And Oxidatively Damaged Dna, Hueiwang Anna Jeng, Ruei-Nian Li, Wen-Yi Lin
Are Semen Quality Parameters Sufficient For Biomonitoring Spermatozoa Dna Integrity And Oxidatively Damaged Dna, Hueiwang Anna Jeng, Ruei-Nian Li, Wen-Yi Lin
Community & Environmental Health Faculty Publications
The present study aimed to investigate the relationship between semen quality parameters and DNA integrity, and determine whether semen quality parameters could serve as a reliable biomarker for monitoring sperm DNA damage. Conventional semen parameters from a total of 202 male human subjects were analyzed. DNA fragmentation and 8-oxo-7,8-dihydro-2′- deoxyguanosine (8-oxoGuo) were used to assess sperm DNA integrity. DNA fragmentation was analyzed by the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and sperm chromatin structure assay (SCSA), while 8-oxodGuo was quantified by the liquid chromatography/tandem mass spectrometry (LC-MS/MS) coupled with an on-line solid phase system. The levels of …