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Articles 1 - 21 of 21
Full-Text Articles in Life Sciences
Protein Recycling In Bering Sea Algal Incubations, Eli K. Moore, H. Rodger Harvey, Jessica F. Faux, David R. Goodlett, Brook L. Nunn
Protein Recycling In Bering Sea Algal Incubations, Eli K. Moore, H. Rodger Harvey, Jessica F. Faux, David R. Goodlett, Brook L. Nunn
OES Faculty Publications
Protein present in phytoplankton represents a large fraction of the organic nitrogen and carbon transported from its synthesis in surface waters to marine sediments. Yet relatively little is known about the longevity of identifiable protein in situ, or the potential modifications to proteins that occur during bloom termination, protein recycling and degradation. To address this knowledge gap, diatom-dominated phytoplankton was collected during the Bering Sea spring blooms of 2009 and 2010, and incubated under darkness in separate shipboard degradation experiments spanning 11 and 53 d, respectively. In each experiment, the protein distribution was monited over time using shotgun proteomics, …
Predation Thresholds In Marine Microbial Communities Applied To Environments With Low Prey Abundances, Bonnie Bailey
Predation Thresholds In Marine Microbial Communities Applied To Environments With Low Prey Abundances, Bonnie Bailey
OES Theses and Dissertations
Prokaryotes (Bacteria and Archaea) comprise the largest component of biomass in the world's oceans. Their abundances are controlled by resource availability, viral infections and protist grazing. Many pico- and nano-eukaryotic predators grow almost as quickly as their prey, and greatly increase in numbers as soon as their prey do, leading in tum to depletion in prokaryotes. It is still unclear however, as to what extent microbial predators are able to feed in low prey environments, most prominently in the largest biome on Earth, the deep sea (below l 000 m depth). It has been hypothesized that in low prey environments, …
A Comprehensive Evaluation Of C25 Highly Branched Isoprenoid Alkenes Of Marine Diatoms As Proxies For Sea Ice Extent In The Arctic Ocean, Tetiana Muniak
A Comprehensive Evaluation Of C25 Highly Branched Isoprenoid Alkenes Of Marine Diatoms As Proxies For Sea Ice Extent In The Arctic Ocean, Tetiana Muniak
OES Theses and Dissertations
Sea Ice extent is one of the major factors regulating carbon cycling and ecosystem function in the modern Arctic Ocean. It is an essential component of climate models and is crucial for the evaluation of various oceanographic processes that influence a particular region. Yet it is also one of the most difficult attributes of the ocean with respect to our ability for its accurate reconstruction from paleo records. The lack of the detailed records prior to satellite information has encouraged the development of new proxy records for the reconstruction of past
sea ice conditions. In recent years, a new monounsaturated …
Spatial And Temporal Variability In Net Community Metabolism In The Middle Atlantic Bight, Georges Bank, And Gulf Of Maine: Observations Of Net Heterotrophic Phases In The Highly Productive Coastal Zone, Cory James Staryk
OES Theses and Dissertations
Net community metabolism (NCM), the balance between primary production and community respiration, was measured during two cruises, June 2011 and August 2012, in the Middle Atlantic Bight, Georges Bank and Gulf of Maine. Net heterotrophy was observed in Georges Bank and Gulf of Maine during the June 2011 cruise. During the August 2012 cruise, net heterotrophy was observed in the Middle Atlantic Bight and net autotrophy was observed in Georges Bank and the Gulf of Maine. Dark community respiration increased (DCR) with biomass and temperature. Gross community production (GCP) also increased with Chl a, bicarbonate uptake, and temperature. Net …
Phytoplankton In Virginia Lakes And Reservoirs: Part Ii, Harold G. Marshall
Phytoplankton In Virginia Lakes And Reservoirs: Part Ii, Harold G. Marshall
Virginia Journal of Science
Phytoplankton composition from 16 Virginia lakes and reservoirs are discussed with emphasis on the dominant taxa, algal bloom producers, and potentially harmful species at these locations. This is a companion study to the more comprehensive publication regarding Virginia freshwater phytoplankton by Marshall (2013).
Comparison Of Two Separation Methods For Biological Particles: Field-Flow Fractionation (Fff) And Sucrose Density Gradients, Cody E. Garrison
Comparison Of Two Separation Methods For Biological Particles: Field-Flow Fractionation (Fff) And Sucrose Density Gradients, Cody E. Garrison
OES Theses and Dissertations
Sucrose gradient centrifugation and Field-flow fractionation (FFF) are two different particle separation methods that overcome the problems of similar size microorganisms clumping together during standard filtration methods. FFF separates particles based on size and density via the parabolic velocity profile of laminar flow in a ribbon-like channel. The sucrose method separates particles via centrifugation in a density gradient. Both techniques worked well in separating eukaryotic from prokaryotic microbes, with the preferred method depending on the type and relative abundance of organisms to be separated. Minicells were separated from mother cells in transformed Escherichia coli cultures, heterotrophic flagellates (e.g., Diplonema papillatum …
Growth And Nitrogen Uptake Kinetics In Cultured Procentrum Donghaiense, Zhangxi Hu, Shunshan Duan, Margaret R. Mulholland
Growth And Nitrogen Uptake Kinetics In Cultured Procentrum Donghaiense, Zhangxi Hu, Shunshan Duan, Margaret R. Mulholland
OES Faculty Publications
We compared growth kinetics of Prorocentrum donghaiense cultures on different nitrogen (N) compounds including nitrate, ammonium urea, glutamic acid, dialanine, and cynate. P. donghaiense exhibited standard Monod-type growth kinetics over a range of nitrogen concentrations for all N. Compounds tested. Cultures grown on glu and urea had the highest maximum growth rates. However, cultures grown on cyanate had lower half saturation constants. Nitrogen uptake kinetics were measured in nitrate-deplete and replete batch cultures of P. donghaiense. In nitrate-deplete batch cultures, P. donghaiense exhibited Michaelis-Menten type uptake kinetics for nitrate, ammonium, urea and algal amino acids; uptake was saturated at …
Estimating Sustainable Harvests Of Eastern Oysters, Crassostrea Virginica, Thomas M. Soniat, Nathan Cooper, Eric N. Powell, John M. Klinck, Mahdi Abdelguerfi, Shengru Tu, Roger Mann, Patrick D. Banks
Estimating Sustainable Harvests Of Eastern Oysters, Crassostrea Virginica, Thomas M. Soniat, Nathan Cooper, Eric N. Powell, John M. Klinck, Mahdi Abdelguerfi, Shengru Tu, Roger Mann, Patrick D. Banks
CCPO Publications
Sustainability of a fishery is traditionally and typically considered achieved if the exploited population does not decline in numbers or biomass over time as a result of fishing relative to biological reference point goals. Oysters, however, exhibit atypical population dynamics compared with many other commercial species. The population dynamics often display extreme natural interannual variation in numbers and biomass, and oysters create their own habitat--the reef itself. With the worldwide decline of oyster reef habitat and the oyster fisheries dependent thereon, the maintenance of shell has received renewed attention as essential to population sustainability. We apply a shell budget model …
Diuron From Maui Sugarcane Field Runoff Is Potentially Harmful To Local Coral Reefs, Thomas C. Royer, Patricia A. Tester, Thomas N. Stewart
Diuron From Maui Sugarcane Field Runoff Is Potentially Harmful To Local Coral Reefs, Thomas C. Royer, Patricia A. Tester, Thomas N. Stewart
CCPO Publications
In a grassroots effort to identify stressors influencing the health of Maui’s coral reefs, samples for the pre- and post-emergent herbicide, Diuron, were taken in July–August 2013, prior to, during, and after the passage of Tropical Storm Flossie on the south central coast of Maui, Hawaii. Diuron is routinely used on sugarcane fields adjacent to Hawaiian coastal waters and is a component of the anti-fouling paint used on small boats. It inhibits photosynthesis of terrestrial plants at concentrations as low as 0.1 parts per billion and can be lethal to endosymbiotic dinoflagellates in corals at concentrations less than 3 parts …
Effects Of Predation Upon The Long-Spined Sea Urchin Diadema Antillarum By The Spotted Spiny Lobster Panulirus Guttatus, Meredith D. Kintzing, Mark J. Butler Iv
Effects Of Predation Upon The Long-Spined Sea Urchin Diadema Antillarum By The Spotted Spiny Lobster Panulirus Guttatus, Meredith D. Kintzing, Mark J. Butler Iv
Biological Sciences Faculty Publications
Sea urchins, important herbivores in marine ecosystems, are strongly impacted by both the direct and indirect effects of predation, and the long-spined sea urchin Diadema antillarum is no exception. Once abundant on Caribbean coral reefs, D. antillarum populations were decimated by disease in the early 1980s, and only where their natural predators have been over-fished has D. antillarum recovery been observed. Spiny lobsters (Palinuridae) are predators of sea urchins, and although there are several species of spiny lobster in the Caribbean, only the spotted spiny lobster Panulirus guttatus is restricted to coral reefs where D. antillarum dwells. We investigated the …
So, You Want To Use Next Generation Sequencing In Marine Systems? Insight From The Pan Pacific Advanced Studies Institute, D. A. Willette, F. W. Allendorf, P. H. Barber, D. J. Barshis, K. E. Carpenter, E. D. Crandall, W. A. Cresko, I. Fernandez-Silva, M. V. Matz, E. Meyer, M. D. Santos, L. W. Seeb, J. E. Seeb
So, You Want To Use Next Generation Sequencing In Marine Systems? Insight From The Pan Pacific Advanced Studies Institute, D. A. Willette, F. W. Allendorf, P. H. Barber, D. J. Barshis, K. E. Carpenter, E. D. Crandall, W. A. Cresko, I. Fernandez-Silva, M. V. Matz, E. Meyer, M. D. Santos, L. W. Seeb, J. E. Seeb
Biological Sciences Faculty Publications
The emerging field of next-generation sequencing (NGS) is rapidly expanding capabilities for cutting edge genomic research, with applications that can help meet marine conservation challenges of food security, biodiversity loss, and climate change. Navigating the use of these tools, however, is complex at best. Furthermore, applications of marine genomic questions are limited in developing nations where both marine biodiversity and threats to marine biodiversity are most concentrated. This is particularly true in Southeast Asia. The first Pan-Pacific Advanced Studies Institute (PacASI) entitled "Genomic Applications to Marine Science and Resource Management in Southeast Asia" was held in July 2012 in Dumaguete, …
Reproductive Phase Determination In Male Meagre (Argyrosomus Regius, Sciaenidae): Testis Development And Histologic Corroboration Of A Gross Anatomical Scale, Nuno Prista, Leonel Gordo, Jose L. Costa, Maria J. Costa, Cynthia M. Jones
Reproductive Phase Determination In Male Meagre (Argyrosomus Regius, Sciaenidae): Testis Development And Histologic Corroboration Of A Gross Anatomical Scale, Nuno Prista, Leonel Gordo, Jose L. Costa, Maria J. Costa, Cynthia M. Jones
OES Faculty Publications
Reproductive stage determination of male gonads has received sparse attention in fish biology literature with few studies detailing the building of gross anatomical-and histologic scales. The meagre (Argyrosomus regius) is one of the world's largest sciaenids and supports a significant regional fishery in European and North African waters whose reproductive patterns are yet to be fully investigated. In the present study, we derive a macroscopic grading system for meagre testis using semi-quantitative graphs that feature the testis variability along the species size range and time of the year. We then describe the histological stages and reproductive phases of …
Lack Of Equivalence In The Elemental And Stable Isotope Chemistry Within The Sagittal Otolith Pair Of The Summer Flounder, Paralichthys Dentatus, Antranik Kajajian, Jason J. Schaffler, Cynthia M. Jones
Lack Of Equivalence In The Elemental And Stable Isotope Chemistry Within The Sagittal Otolith Pair Of The Summer Flounder, Paralichthys Dentatus, Antranik Kajajian, Jason J. Schaffler, Cynthia M. Jones
OES Faculty Publications
In fish that are not bilaterally symmetrical, the left and right sagittae are often not symmetrical, exhibiting divergent growth patterns and mass, and may have differences in chemical composition. We investigated this in the asymmetrical summer flounder Paralichthys dentatus, collected from different nursery habitats along the US east coast. Significant differences were detected in otolith mass, δ13C, δ18O, Li:Ca, Mg:Ca, and Sr:Ca, and overall chemical signatures. These results refute the hypothesis of left-right equivalence that is prevalent for bilaterally symmetrical fishes. We tested whether a specific side was better suited for classification. The best models …
Krill, Climate, And Contrasting Future Scenarios For Arctic And Antarctic Fisheries, Margaret M. Mcbride, Padmini Dalpadado, Kenneth F. Drinkwater, Olav Rune Godø, Alistair J. Hobday, Anne B. Hollowed, Trond Kristiansen, Eugene J. Murphy, Patrick H. Ressler, Sam Subbey, Eileen E. Hofmann, Harald Loeng
Krill, Climate, And Contrasting Future Scenarios For Arctic And Antarctic Fisheries, Margaret M. Mcbride, Padmini Dalpadado, Kenneth F. Drinkwater, Olav Rune Godø, Alistair J. Hobday, Anne B. Hollowed, Trond Kristiansen, Eugene J. Murphy, Patrick H. Ressler, Sam Subbey, Eileen E. Hofmann, Harald Loeng
CCPO Publications
Arctic and Antarcticmarine systems have incommon high latitudes, large seasonal changes in light levels, cold air and sea temperatures, and sea ice. In other ways, however, they are strikingly different, including their: age, extent, geological structure, ice stability, and foodweb structure. Both regions contain very rapidly warming areas and climate impacts have been reported, as have dramatic future projections. However, the combined effects of a changing climate on oceanographic processes and foodweb dynamics are likely to influence their future fisheries in very different ways. Differences in the life-history strategies of the key zooplankton species (Antarctic krill in the Southern Ocean …
Emergence Of Algal Blooms: The Effects Of Short-Term Variability In Water Quality On Phytoplankton Abundance, Diversity, And Community Composition In A Tidal Estuary, Todd A. Egerton, Ryan E. Morse, Harold G. Marshall, Margaret R. Mulholland
Emergence Of Algal Blooms: The Effects Of Short-Term Variability In Water Quality On Phytoplankton Abundance, Diversity, And Community Composition In A Tidal Estuary, Todd A. Egerton, Ryan E. Morse, Harold G. Marshall, Margaret R. Mulholland
OES Faculty Publications
Algal blooms are dynamic phenomena, often attributed to environmental parameters that vary on short timescales (e.g., hours to days). Phytoplankton monitoring programs are largely designed to examine long-term trends and interannual variability. In order to better understand and evaluate the relationships between water quality variables and the genesis of algal blooms, daily samples were collected over a 34 day period in the eutrophic Lafayette River, a tidal tributary within Chesapeake Bay's estuarine complex, during spring 2006. During this period two distinct algal blooms occurred; the first was a cryptomonad bloom and this was followed by a bloom of the mixotrophic …
Detection Of Seagrass Scars Using Sparse Coding And Morphological Filter, Ender Oguslu, Sertan Erkanli, Victoria J. Hill, W. Paul Bissett, Richard C. Zimmerman, Jiang Li, Charles R. Bostater Jr. (Ed.), Stelios P. Mertikas (Ed.), Xavier Neyt (Ed.)
Detection Of Seagrass Scars Using Sparse Coding And Morphological Filter, Ender Oguslu, Sertan Erkanli, Victoria J. Hill, W. Paul Bissett, Richard C. Zimmerman, Jiang Li, Charles R. Bostater Jr. (Ed.), Stelios P. Mertikas (Ed.), Xavier Neyt (Ed.)
OES Faculty Publications
We present a two-step algorithm for the detection of seafloor propeller seagrass scars in shallow water using panchromatic images. The first step is to classify image pixels into scar and non-scar categories based on a sparse coding algorithm. The first step produces an initial scar map in which false positive scar pixels may be present. In the second step, local orientation of each detected scar pixel is computed using the morphological directional profile, which is defined as outputs of a directional filter with a varying orientation parameter. The profile is then utilized to eliminate false positives and generate the final …
Evolution Of The Freshwater Sardinella, Sardinella Tawilis (Clupeiformes: Clupeidae), In Taal Lake, Philippines And Identification Of Iits Marine Sister-Species, Sardinella Hualiensis, Demian Willette, Kent E. Carpenter, Mudjekeewis Santos
Evolution Of The Freshwater Sardinella, Sardinella Tawilis (Clupeiformes: Clupeidae), In Taal Lake, Philippines And Identification Of Iits Marine Sister-Species, Sardinella Hualiensis, Demian Willette, Kent E. Carpenter, Mudjekeewis Santos
Biological Sciences Faculty Publications
We identify the sister species of the world's only freshwater sardinella, Sardinella tawilis (Herre, 1927) of Taal Lake, Philippines as the morphologically-similar marine Taiwanese sardinella Sardinella hualiensis (Chu and Tsai, 1958). Evidence of incomplete lineage sorting and a species tree derived from three mitochondrial genes and one nuclear gene indicate that S. tawilis diverged from S. hualiensis in the late Pleistocene. Neutrality tests, mismatch distribution analysis, sequence diversity indices, and species tree analysis indicate populations of both species have long been stable and that the divergence between these two lineages occurred prior to the putative 18th century formation of Taal …
The Impact Of Multiple Nursery Areas On The Population Structure Of Atlantic Menhaden, Brevooria Tyrannus, Kristen A. Anstead
The Impact Of Multiple Nursery Areas On The Population Structure Of Atlantic Menhaden, Brevooria Tyrannus, Kristen A. Anstead
OES Theses and Dissertations
Understanding the population structure and patterns of connectivity in marine fishes is essential when making predictions about a species' resiliency and persistence in an increasingly changing environment. The Atlantic Menhaden Brevoortia tyrannus is a clupeid that plays a critical role in the marine food web and supports one of the largest fisheries on the US East Coast. In addition to a decrease in overall numbers and spawning stock biomass, recruitment levels have remained low since the 1990s. Menhaden use numerous estuaries along the Atlantic coast for juvenile development before recruiting to the adult population and the contribution of each of …
Phytoplankton And Nutrient Dynamics In A Tidally Dominated Eutrophic Estuary: Daily Variability And Controls On Bloom Formation, Ryan E. Morse, Margaret R. Mulholland, Todd A. Egerton, Harold G. Marshall
Phytoplankton And Nutrient Dynamics In A Tidally Dominated Eutrophic Estuary: Daily Variability And Controls On Bloom Formation, Ryan E. Morse, Margaret R. Mulholland, Todd A. Egerton, Harold G. Marshall
OES Faculty Publications
To better understand nutrient dynamics and factors that promote the initiation of algal blooms, the Lafayette River, a tidal subestuary of Chesapeake Bay that experiences seasonal algal blooms, was sampled daily for a period of 54 d in the fall of 2005. Three phytoplankton blooms (chl a concentrations exceeding twice the average of monthly measurements from 2000 to 2009) occurred during this period: a mixed bloom of Akashiwo sanguinea and Gymnodinium sp., a monospecific Skeletonema costatum bloom, and a monospecific Gymnodinium sp. bloom. Over the sampling period, nutrient concentrations increased following precipitation events and were elevated between bloom periods but …
Reproductive Phase Determination In Male Meagre (Argyrosomus Regius, Sciaenidae): Testis Development And Histologic Corroboration Of A Gross Anatomical Scale, Nuno Prista, Leonel Gordo, José Lino Costa, Maria José Costa, Cynthia Jones
Reproductive Phase Determination In Male Meagre (Argyrosomus Regius, Sciaenidae): Testis Development And Histologic Corroboration Of A Gross Anatomical Scale, Nuno Prista, Leonel Gordo, José Lino Costa, Maria José Costa, Cynthia Jones
OES Faculty Publications
Reproductive stage determination of male gonads has received sparse attention in fish biology literature with few studies detailing the building of gross anatomical- and histologic scales. The meagre (Argyrosomus regius) is one of the world's largest sciaenids and supports a significant regional fishery in European and North African waters whose reproductive patterns are yet to be fully investigated. In the present study, we derive a macroscopic grading system for meagre testis using semi-quantitative graphs that feature the testis variability along the species size range and time of the year. We then describe the histological stages and reproductive phases of male …
Can We Predict The Future: Juvenile Finfish And Their Seagrass Nurseries In The Chesapeake Bay, Cynthia M. Jones
Can We Predict The Future: Juvenile Finfish And Their Seagrass Nurseries In The Chesapeake Bay, Cynthia M. Jones
OES Faculty Publications
The importance of estuarine seagrass beds as nurseries for juvenile fish has become a universal paradigm, especially for estuaries that are as important as the Chesapeake Bay. Yet, scientific tests of this hypothesis were equivocal depending on species, location, and metrics. Moreover, seagrasses themselves are under threat and one-third of seagrasses have disappeared worldwide with 65 of their losses occurring in estuaries. Although there have been extensive studies of seagrasses in the Chesapeake Bay, surprisingly few studies have quantified the relationship between seagrass as nurseries for finfish in the Bay. Of the few studies that have directly evaluated the use …