Life Sciences Commons^™

Prokaryotic Diversity Of The Wastewater Outfalls, Reefs, And Inlets Of Broward County, Alexandra Mandina Campbell

HCNSO Student Theses and Dissertations

We applied culture-independent, next-generation sequencing (NGS) high throughput pyrosequencing, to characterize the microbial communities associated with near shore seawater in Broward County, FL. These waters flow over coral reef communities, which are part of the Florida reef tract, and are close to shore where bathers frequent. Through a close partnership with the NOAA FACE program, 38 total seawater samples were taken from 6 distinct locales -the Port Everglades and Hillsboro Inlets, Hollywood and Broward wastewater outfalls, and the associated reef waters-over the course of one year. Tagged 16S rRNA amplicons were used to generate longitudinal taxonomic profiles of marine bacteria …