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Life Sciences Commons

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Nova Southeastern University

Oceanography and Atmospheric Sciences and Meteorology

HCNSO Student Theses and Dissertations

16S

Publication Year

Articles 1 - 4 of 4

Full-Text Articles in Life Sciences

The Role Of Bacterial Symbionts And Bioluminescence In The Pyrosome, Pyrosoma Atlanticum, Alexis Berger May 2020

The Role Of Bacterial Symbionts And Bioluminescence In The Pyrosome, Pyrosoma Atlanticum, Alexis Berger

HCNSO Student Theses and Dissertations

The pelagic tunicate, Pyrosoma atlanticum, is known for its brilliant bioluminescence, but the mechanism causing this bioluminescence has not been fully characterized. This study identifies the bacterial bioluminescent symbionts of P. atlanticum collected in the northern Gulf of Mexico using various methods such as electron microscopy, light microscopy, and molecular genetics. The bacteria are localized within a specific pyrosome light organ. Bioluminescent symbiotic bacteria of Vibrionaceae composed >50% of taxa in tunicate samples (n=13), which was shown by utilizing current molecular genetics methodologies. While searching for bacterial lux genes in 2 tunicate samples, we also serendipitously generated a draft …


Characterization Of Bacterial Communities In Biscayne Bay Through Genomic Analysis, Eric Fortman Dec 2019

Characterization Of Bacterial Communities In Biscayne Bay Through Genomic Analysis, Eric Fortman

HCNSO Student Theses and Dissertations

Biscayne Bay is a shallow oligotrophic estuary in Southeast Florida. Channelization of rivers, and dredging of canals has greatly altered the historical flow of fresh water into the bay. This, coupled with the rise of a sprawling urban & suburban development, has greatly increased the nutrient load in the bay. This study examined the bacterial community at 14 stations throughout Biscayne Bay —6 stations were located at the mouths of canals; 1 upstream-canal station; 6 stations in the center of the bay; and one ocean influenced station, located near the entrance to the bay. One liter, surface water samples were …


Inferred Function And Dynamics Of Microbial Communities From The Northern Gulf Of Mexico, Deepesh Tourani Nov 2019

Inferred Function And Dynamics Of Microbial Communities From The Northern Gulf Of Mexico, Deepesh Tourani

HCNSO Student Theses and Dissertations

Microbial communities, or microbiomes, are the major drivers of global biogeochemical cycles, acting as primary producers and decomposers across the water column in the oceans. Thus, they reflect changes in physicochemical properties and nutrient composition of the ocean. However, this correlation between ecological changes and the function of marine microbiomes is poorly understood. Large-scale oceanic events such as the bottom-water oxygen-depleted zone (i.e., “dead zone”) and the Deepwater Horizon oil spill in the Gulf of Mexico (GoM) render the ecosystem fragile. These events decrease survival rates of pelagic and coastal macrofauna and affect the biodiversity of the region. As part …


Fine-Grained Bacterial Compositional Analsysis Of The Port Everglades Inlet (Broward County, Fl) Microbiome Using High Throughput Dna Sequencing, Lauren M. O'Connell Oct 2015

Fine-Grained Bacterial Compositional Analsysis Of The Port Everglades Inlet (Broward County, Fl) Microbiome Using High Throughput Dna Sequencing, Lauren M. O'Connell

HCNSO Student Theses and Dissertations

Port Everglades Inlet is one of the busiest ports in the country and is a point source of pollution to surrounding beaches and offshore corals from heavy boat traffic and urban runoff. Understanding fluctuations of bacterioplankton communities in major port inlets is important due to their impacts on surrounding marine environments. To understand annual microbial fluctuations, the 16s rRNA V4 hypervariable region was sequenced using Illumina high-throughput DNA sequencing technology. Surface samples were taken weekly for one year to generate baseline fluctuations in the microbial community. Total reads of 1.4 million were generated with a final count of 16,384 Operational …