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Full-Text Articles in Life Sciences
The Role Of Skeletal Muscle-Synthesized Brain Derived Neurotrophic Factor In The Maintenance Of Motor Neuron Mitochondrial Populations, Mikel Cawley
All NMU Master's Theses
Mitochondria are essential for the high energy demands of the neuromuscular junction and, as a consequence, leave motorneurons susceptible to dysfunction. A potential origin of progressive pathology may be a reduction in brain-derived neurotrophic-factor (BDNF) signaling at the motor unit. We have shown that mice deficient in skeletal muscle-synthesized BDNF (msBDNF) demonstrate progressive motorneuron and muscle pathology at 120d. We hypothesize mitochondrial populations will be altered in motorneurons of msBDNF deficient-mice. At 117d, msBDNF deficient-mice received intramuscular injections of MitoTracker™ dye targeting the right gastrocnemius muscle. At 120d experimental groups underwent a gastrocnemius harvest or a sciatic nerve ligation protocol …
Serum-Based Biomarkers And Magnetic Resonance Imaging Following Mild Traumatic Brain Injury In Collegiate Athletes Post Return-To-Play, Taylor R. Susa
Serum-Based Biomarkers And Magnetic Resonance Imaging Following Mild Traumatic Brain Injury In Collegiate Athletes Post Return-To-Play, Taylor R. Susa
All NMU Master's Theses
Recently there has been an increase in the use of MRI (Magnetic Resonance Imaging), to measure the effects of traumatic brain injury (TBI). Proteins such as BDNF, S100B, UCH-L1, and Tau have been found to have altered levels in blood serum after TBI. However, there is limited knowledge about the relationship between serum-based and MRI-based biomarkers in concussed athletes post return-to-play. This study aimed to bridge this gap by collecting serum samples from 42 participants across two groups. The first group (n = 21) consisted of recently cleared to return-to-play collegiate athletes after experiencing a sports-related concussion. The second group …
The Characterization Of Behavioral Abnormalities In Bdnf Loxp Transgenic Mice, Ryan Brandt
The Characterization Of Behavioral Abnormalities In Bdnf Loxp Transgenic Mice, Ryan Brandt
All NMU Master's Theses
BDNF homozygous floxed mice (BDNF lox+/+) are a transgenic mouse strain used to study the neurotrophin brain-derived neurotrophic factor (BDNF) through Cre-Lox recombination when crossed with the appropriate Cre-expressing strain. BDNF lox+/+ mice contain two artificially inserted LoxP sites located upstream and downstream from the BDNF coding region. This strain was originally described as physiologically normal and fertile by Rios et al., (2001). However, current literature lacks sufficient characterization and description of its behavioral phenotype. We utilized a three-stage behavioral protocol which included home cage monitoring observations, open-field, tail suspension, and acoustic PPI to provide a detailed behavioral phenotype …
Characterization Of The Roles Of Muscle-Synthesized Brain-Derived Neurotrophic Factor And Presynaptic Tyrosine Receptor Kinase B In Motor Neuron Axonal Transport, Luke A. Vanosdol
Characterization Of The Roles Of Muscle-Synthesized Brain-Derived Neurotrophic Factor And Presynaptic Tyrosine Receptor Kinase B In Motor Neuron Axonal Transport, Luke A. Vanosdol
All NMU Master's Theses
Brain-derived neurotrophic factor (BDNF) is a small, diffusible protein essential for the development and function of neurons. It is synthesized by many types of tissue, including muscle. BDNF actions are mediated via binding to its receptor, tyrosine receptor kinase B (TrkB). The BDNF-TrkB complex is endocytosed into a specialized vesicle, which induces downstream signaling cascades locally in the dendrites, or, more often, is delivered to the cell soma via retrograde axonal transport, where it modulates gene expression. BDNF activation of TrkB is critical for the initiation of axonal transport, and this cellular process relies on the interaction of numerous adaptor …
The Role Of Skeletal Muscle-Synthesized Brain-Derived Neurotrophic Factor In Retrograde Transport Along Motorneuron Axons, Rebecca L. Dangremond
The Role Of Skeletal Muscle-Synthesized Brain-Derived Neurotrophic Factor In Retrograde Transport Along Motorneuron Axons, Rebecca L. Dangremond
All NMU Master's Theses
Brain-derived neurotrophic factor (BDNF) is a neurotrophin required for the differentiation, growth, and survival of neurons. BDNF is synthesized in several tissue types including skeletal muscle. Neuromuscular diseases (NMDs) are characterized by the degeneration of motorneurons and/or associated skeletal muscles, and reduced BDNF is implicated in NMDs. Using Cre-Lox gene recombination technology our lab generated transgenic mice in which the BDNF gene was knocked out of skeletal muscle. We hypothesized that skeletal muscle-BDNF is required for normal retrograde transport of proteins and mitochondria along gastrocnemius-associated motorneuron axons. We first measured accumulation of the retrogradely transported phosphorylated neurofilament-H (NF-H-P) protein at …