Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Life Sciences
Fragmentation And Characteristics Of Recombinant Endochitinase Expressed In Escherichia Coli, Wei-Ming Chen, Gen-Hung Chen, Shann-Tzong Jiang
Fragmentation And Characteristics Of Recombinant Endochitinase Expressed In Escherichia Coli, Wei-Ming Chen, Gen-Hung Chen, Shann-Tzong Jiang
Journal of Marine Science and Technology
A recombinant endochitinase fused with hexahistidine (rEC-H) was expressed and characterized. After Ni affinity chromatography, the recovery, purification-fold and specific activity of rEC-H were 88.8%, 13.4 and 142.1 U/mg, respectively. The purified rEC-H had optimal pH and temperature at pH 7.5 and 60°C, respectively and was stable at pH 4.0-9.0 and