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Articles 1 - 11 of 11
Full-Text Articles in Life Sciences
Survey Of The Great Kanawha River, West Virginia, For Virulence Related Gene Markers Stx1, Stx2, And Eaea, Christina Carole Johnson
Survey Of The Great Kanawha River, West Virginia, For Virulence Related Gene Markers Stx1, Stx2, And Eaea, Christina Carole Johnson
Theses, Dissertations and Capstones
Microbial surveying using antibiotic resistant bacteria, fecal coliforms, and virulence genes is an approach not previously tested on the Great Kanawha River. Research objectives were to test antibiotic resistant and fecal coliform bacteria as bioindicators of water quality, and develop a multiplex- polymerase chain reaction (mPCR) system for identification of stx1, stx2, and eaeA DNA sequences in isolated fecal coliforms (FC). Fecal indicator populations and antibiotic resistant populations were demonstrated to be independent. Bacterial populations were assigned impact score(s) (IS) values for each sample site based on data set percent ranks. Population scores were summed per sample site to generate …
Chmp1 Is Implicated In The Development Of Pancreatic Tumor Via The Retinoic Acid Signaling Pathway, Juliana Adedayo Akinsete
Chmp1 Is Implicated In The Development Of Pancreatic Tumor Via The Retinoic Acid Signaling Pathway, Juliana Adedayo Akinsete
Theses, Dissertations and Capstones
In the present study, we investigated the involvement of Chmp1 (Chromatin ModifyingProtein 1/Charged Multivesicular body Protein 1) in the development of mousepancreatic acinar tumor cell line. CRL 2151 cell line was transfected with Chmp1/CS2vector to compare growth, morphology and expression of Chmp1, p53 and pp53 (ser37) with control-transfected cells. CRL 2151 cells were treated with all-trans retinoicacid (ATRA) to compare growth, morphology and expression of Chmp1 and p53 withcontrol-treated cells. Strabismus was used as control. Results showed inhibition ofgrowth but no morphological change in transfected cells. Western blot analysis showedthat Chmp1 transfection upregulated the expression of p53, pp53 and Stbm …
The Breeding Ecology And Natural History Of Ambystomatid Salamanders In An Ephemeral Wetland In Mason County, West Virginia, S. Douglas Kaylor
The Breeding Ecology And Natural History Of Ambystomatid Salamanders In An Ephemeral Wetland In Mason County, West Virginia, S. Douglas Kaylor
Theses, Dissertations and Capstones
A forested ephemeral wetland in Mason County, WV, documented to contain 4 of 5 Ambystoma species found in the state, was studied to learn about population sizes, breeding cycles, and habitat use. Minnow traps were placed along three drift fences with additional traps placed throughout the study area. From February 5 to March 27, 2005, 85 captured adults were identified to species, marked by elastomer injection for mark-recapture analysis, and measured for morphometrics. Larval salamanders were identified, staged, measured, and returned. Egg clutches were mapped and counted. Mark-recapture analysis suggests the A. texanum population size is between 635 and 735 …
The Zucker Rat As A Model Of Obesity-Hypertension, Ryan Morrison
The Zucker Rat As A Model Of Obesity-Hypertension, Ryan Morrison
Theses, Dissertations and Capstones
Hypertension is a serious health problem that affects approximately 1 in 4 American adults. Most cases are diagnosed as essential hypertension, meaning that the exact cause is unknown. In most patients, however, excess weight is a major contributory factor to the development of essential hypertension. The role of obesity in promoting hypertension is now well documented and has become the foundation for an entire field of research known alternately as obesity-hypertension, obesity-induced hypertension, or obesity-associated hypertension. In this field, rapid advances are being made in our understanding of how obesity and hypertension are linked. A plethora of related risk factors, …
Antibiotic Resistant And Coliform Bacteria In The Ohio River; 2002 To 2004, Lisa Marie Smith
Antibiotic Resistant And Coliform Bacteria In The Ohio River; 2002 To 2004, Lisa Marie Smith
Theses, Dissertations and Capstones
During 2002 and 2003 samples, collected every five miles along the Ohio River, were analyzed for total cultivable bacteria, ciprofloxacin resistant bacteria, erythromycin resistant bacteria, tetracycline resistant bacteria, and fecal coliforms. During 2004 samples were analyzed for total cultivable bacteria, ciprofloxacin resistant bacteria, sulfamethizole resistant bacteria, tetracycline resistant bacteria, Virginiamycin resistant bacteria, total coliforms, and Escherichia coli. The objectives of this study were to systematically collect data on fecal coliforms, E. coli, and antibiotic resistant bacteria in the Ohio River and its major tributaries; to determine if antibiotic resistance populations are correlated to each other or to coliforms; and …
Occurrence And Distribution Of Multi-Antibiotic Resistant Bacteria From The Great Kanawha River, West Virginia, April Dawn Keenan
Occurrence And Distribution Of Multi-Antibiotic Resistant Bacteria From The Great Kanawha River, West Virginia, April Dawn Keenan
Theses, Dissertations and Capstones
During the spring and summer of 2004 subsurface mid-channel samples were collected from the Kanawha River and its five primary tributaries (New, Gauley, Elk, Coal and Pocatalico Rivers). The first two objectives of this study were to enumerate bacteria resistant to ciprofloxacin, erythromycin or tetracycline, and test them for multiple resistance to seven commonly used antibiotics. The third objective was to determine the Minimum Inhibitory Concentration (MIC) for seven antibiotics starting at concentrations 20 times the published working concentrations for Gram-negative bacteria. The final objective of this study was to determine if a novel Impact Scoring system incorporating a current …
Characterization Of Vinyl Chloride Degrading Bacteria From A Serial Anaerobic-Aerobic Chloroethene Bioreactor, Fredrick Heath Damron
Characterization Of Vinyl Chloride Degrading Bacteria From A Serial Anaerobic-Aerobic Chloroethene Bioreactor, Fredrick Heath Damron
Theses, Dissertations and Capstones
Chloroethenes are among the most common groundwater contaminants in the United States. In anoxic groundwater, chloroethenes can be reduced to vinyl chloride (VC), a known carcinogen. The goal of this research was to characterize bacteria that could mineralize VC from a microbial community within a serial aerobic-anaerobic chloroethene bioreactor. Isolations for organisms capable of VC degradation were performed. The ability to degrade VC was first indicated by accumulation of chloride. Gas chromatography assay showed that VC was degraded in cultures capable of chloride accumulation. The epoxyalkane:coenzyme M transferase (EaCoMT) gene which codes for an enzyme previously shown to function in …
Natural History And Distribution Of The Upland Chorus Frog, Pseudacris Feriarum Baird, In West Virginia, Jaime Sias
Natural History And Distribution Of The Upland Chorus Frog, Pseudacris Feriarum Baird, In West Virginia, Jaime Sias
Theses, Dissertations and Capstones
Chapter 1 is a literature review of Pseudacris feriarum, largely based on published P. triseriata Complex species accounts. Chapter 2 presents the natural history of P. feriarum in West Virginia and compares some parameters with other Pseudacris species. Chapter 3 looks at the phenology of a wetland in eastern West Virginia. Chapter 4 examines the current range of P. feriarum and compares it with the historical range in the state. Finally, hypotheses are given as to why Upland Chorus Frogs have declined in West Virginia. All information obtained from this study should be used to create a management plan for …
The Natural History And Thermal Ecology Of A Population Of Spotted Turtles (Clemmys Guttata) And Wood Turtles (Glyptemys Insculpta) In West Virginia, Ariana N. Breisch
The Natural History And Thermal Ecology Of A Population Of Spotted Turtles (Clemmys Guttata) And Wood Turtles (Glyptemys Insculpta) In West Virginia, Ariana N. Breisch
Theses, Dissertations and Capstones
An ecological study was conducted from 19 March 2001 to 2 April 2003 on a population of Spotted Turtles (Clemmys guttata) and Wood Turtles (Glyptemys insculpta) in West Virginia. Live-trapping, mark-recapture, radio telemetry and temperature data logging techniques were used to gather information on morphometrics, capture success, movements, home range, behavior, and thermal regime during activity and over-wintering seasons. Twenty-one Spotted Turtles were captured 260 times. Population structure was 42.9% juvenile, 38.1% male, and 19.0% female. Mean home range was 0.52 ha. Fifty Wood Turtles were captured 230 times. Juveniles were 36%, and males and females were 32% each. Mean …
Constructed Ponds As Mitigated Habitat For The Wood Frog (Rana Sylvatica Leconte) And The Spotted Salamander (Ambystoma Maculatum Shaw) In West Virginia, Celeste Dawn Good
Constructed Ponds As Mitigated Habitat For The Wood Frog (Rana Sylvatica Leconte) And The Spotted Salamander (Ambystoma Maculatum Shaw) In West Virginia, Celeste Dawn Good
Theses, Dissertations and Capstones
Many forest dwelling amphibians depend upon aquatic breeding habitats, making them susceptible to habitat changes. To determine if amphibian use of temporary pools occurred, 9 ponds were constructed in 3 forested areas on the MeadWestvaco Wildlife and Ecosystem Research Forest. Studies were conducted in 6 ponds during 2004, and all 9 in 2005 using drift fences. Trapped amphibians were measured and given a pond specific mark with visible implant elastomers. A significant difference was found between low and high elevation sites for juvenile R. sylvatica snout-to-vent length. No significant differences were found for soil, air or water temperatures between sites …
Characterization Of The Dna-Binding Properties Of Silent Information Regulator 3 Protein, Cotteka Nichisha Johnson
Characterization Of The Dna-Binding Properties Of Silent Information Regulator 3 Protein, Cotteka Nichisha Johnson
Theses, Dissertations and Capstones
The eukaryotic genome is organized into distinct domains with discrete regulatory potential, influenced by the variation in local chromatin structure. An example of chromatin-mediated consequences on gene expression is mating type determination of yeast Saccharomyces cerevisiae, which depends on silencing of specific gene cassettes. Silencing in yeast involves key proteins, amongst which the Silent Information Regulator (Sir) proteins are essential. Sir1p, Sir2p, and Sir4p are recruited to nucleate silencing events, while Sir3p serves as the pivotal factor in spreading the silent state. This investigation characterizes the DNA binding properties of Sir3p using DNA fragments with varying degrees of inherent flexibility, …