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Full-Text Articles in Life Sciences
Reversible Deficiencies In Flagellar Beating And Axonemal Assembly, Mei Wei
Reversible Deficiencies In Flagellar Beating And Axonemal Assembly, Mei Wei
Dissertations (1934 -)
Axonemal complexes in flagella are largely prepackaged in the cell body. As such, one mutation often results in the absence of the co-assembled components and permanent motility deficiencies. For example, a Chlamydomonas mutant defective in RSP4 in the radial spoke, which is critical for bend propagation, has paralyzed flagella that also lack the paralogue RSP6 and three additional radial spoke proteins. Intriguingly, recent studies showed that several mutant strains contain a mixed population of swimmers and paralyzed cells despite their identical genetic background. Here we report a cause underlying these variations. Two new mutants lacking RSP6 swim processively and other …
Role Of Dpy-30 Proteins In Flagella, Radhika Gopal
Role Of Dpy-30 Proteins In Flagella, Radhika Gopal
Dissertations (1934 -)
Cilia and flagella are similar organelles found in eukaryotic cells. A microtubule-based axonemal cytoskeleton supports these organelles that project into the extracellular environment to detect various stimuli and propel fluid. These functions enable cells to sense and respond to the changing environment. Because of their importance, the fundamental mechanisms have been conserved throughout evolution. However, it remains largely unknown how axonemes are assembled and how the motility is regulated. This thesis investigated two molecules, RSP2 and RSP23, positioned in the axonemes within a T-shaped complex, the Radial Spoke (RS). Among several axonemal complexes, flagella lacking the evolutionarily conserved RS are …