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Full-Text Articles in Life Sciences
Sperm Fate Is Promoted By The Mir-44 Microrna Family In The Caenorhabditis Elegans Hermaphrodite Germline, Katherine Ann Maniates, Benjamin S. Olson, Allison L. Abbott
Sperm Fate Is Promoted By The Mir-44 Microrna Family In The Caenorhabditis Elegans Hermaphrodite Germline, Katherine Ann Maniates, Benjamin S. Olson, Allison L. Abbott
Biological Sciences Faculty Research and Publications
Posttranscriptional regulation of gene expression, typically effected by RNA-binding proteins, microRNAs (miRNAs), and translation initiation factors, is essential for normal germ cell function. Numerous miRNAs have been detected in the germline; however, the functions of specific miRNAs remain largely unknown. Functions of miRNAs have been difficult to determine as miRNAs often modestly repress target mRNAs and are suggested to sculpt or fine tune gene expression to allow for the robust expression of cell fates. In Caenorhabditis elegans hermaphrodites, cell fate decisions are made for germline sex determination during larval development when sperm are generated in a short window before the …
Functional Analysis Of Microrna Pathway Genes In The Somatic Gonad And Germ Cells During Ovulation In C. Elegans, Carmela Rios, David Warren, Benjamin Olson, Allison L. Abbott
Functional Analysis Of Microrna Pathway Genes In The Somatic Gonad And Germ Cells During Ovulation In C. Elegans, Carmela Rios, David Warren, Benjamin Olson, Allison L. Abbott
Biological Sciences Faculty Research and Publications
MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression that play critical roles in animal development and physiology, though functions for most miRNAs remain unknown. Worms with reduced miRNA biogenesis due to loss of Drosha or Pasha/DGCR8 activity are sterile and fail to ovulate, indicating that miRNAs are required for the process of oocyte maturation and ovulation. Starting with this penetrant sterile phenotype and using new strains created to perform tissue specific RNAi, we characterized the roles of the C. elegans Pasha, pash-1, and two miRNA-specific Argonautes, alg-1 and alg-2, in somatic gonad cells and in germ cells in the regulation …
Stage-Specific Timing Of The Microrna Regulation Of Lin-28 By The Heterochronic Gene Lin-14 In Caenorhabditis Elegans, Jennifer Tsialikas, Mitchell A. Romens, Allison L. Abbott, Eric G. Moss
Stage-Specific Timing Of The Microrna Regulation Of Lin-28 By The Heterochronic Gene Lin-14 In Caenorhabditis Elegans, Jennifer Tsialikas, Mitchell A. Romens, Allison L. Abbott, Eric G. Moss
Biological Sciences Faculty Research and Publications
In normal development, the order and synchrony of diverse developmental events must be explicitly controlled. In the nematode Caenorhabditis elegans, the timing of larval events is regulated by hierarchy of proteins and microRNAs (miRNAs) known as the heterochronic pathway. These regulators are organized in feedforward and feedback interactions to form a robust mechanism for specifying the timing and execution of cell fates at successive stages. One member of this pathway is the RNA binding protein LIN-28, which promotes pluripotency and cell fate decisions in successive stages. Two genetic circuits control LIN-28 abundance: it is negatively regulated by the miRNA …
Most Caenorhabditis Elegans Micrornas Are Individually Not Essential For Development Or Viability, Eric A. Miska, Ezequiel Alvarez-Saavedra, Allison L. Abbott, Nelson C. Lau, Andrew B. Hellman, Shannon M. Mcgonagle, David P. Bartel, Victor R. Ambros, H. Robert Horvitz
Most Caenorhabditis Elegans Micrornas Are Individually Not Essential For Development Or Viability, Eric A. Miska, Ezequiel Alvarez-Saavedra, Allison L. Abbott, Nelson C. Lau, Andrew B. Hellman, Shannon M. Mcgonagle, David P. Bartel, Victor R. Ambros, H. Robert Horvitz
Biological Sciences Faculty Research and Publications
MicroRNAs (miRNAs), a large class of short noncoding RNAs found in many plants and animals, often act to post-transcriptionally inhibit gene expression. We report the generation of deletion mutations in 87 miRNA genes in Caenorhabditis elegans, expanding the number of mutated miRNA genes to 95, or 83% of known C. elegans miRNAs. We find that the majority of miRNAs are not essential for the viability or development of C. elegans, and mutations in most miRNA genes do not result in grossly abnormal phenotypes. These observations are consistent with the hypothesis that there is significant functional redundancy among miRNAs …