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Full-Text Articles in Life Sciences
Effect Of Thermal Mitigation On Porcine Epidemic Diarrhea Virus (Pedv)- Contaminated Feed, R. A. Cochrane, L. L. Schumacher, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, Qi Chen, Jianqiang Zhang, Phillip Charles Gauger, Rodger G. Main, C. K. Jones
Effect Of Thermal Mitigation On Porcine Epidemic Diarrhea Virus (Pedv)- Contaminated Feed, R. A. Cochrane, L. L. Schumacher, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, Qi Chen, Jianqiang Zhang, Phillip Charles Gauger, Rodger G. Main, C. K. Jones
Kansas Agricultural Experiment Station Research Reports
Porcine Epidemic Diarrhea Virus (PEDV) is primarily transmitted by fecal-oral contamination. However, epidemiological evidence has shown that swine feed and ingredients may serve as potential vectors of transmission. Since it is known that PEDV is a heat-sensitive virus, we hypothesized that a conditioner and pellet mill mimicking commercial thermal processing would mitigate PEDV infectivity. To test this hypothesis, two experiments were designed to determine if different pellet mill conditioner retention times or temperatures would impact PEDV infectivity determined by polymerase chain reaction (PCR) analysis and bioassay. For the first study, a 3×3×2 factorial was utilized, with three pelleting temperatures (155, …
Determining The Minimum Infectious Dose Of Porcine Epidemic Diarrhea Virus (Pedv) In A Feed Matrix, L. L. Schumacher, J. C. Woodworth, C. R. Stark, C. K. Jones, R. A. Hesse, Rodger G. Main, Jianqiang Zhang, Phillip Charles Gauger, S. S. Dritz, M. D. Tokach
Determining The Minimum Infectious Dose Of Porcine Epidemic Diarrhea Virus (Pedv) In A Feed Matrix, L. L. Schumacher, J. C. Woodworth, C. R. Stark, C. K. Jones, R. A. Hesse, Rodger G. Main, Jianqiang Zhang, Phillip Charles Gauger, S. S. Dritz, M. D. Tokach
Kansas Agricultural Experiment Station Research Reports
Understanding the magnitude of transmissible risk Porcine Epidemic Diarrhea Virus (PEDV)-infected feed imposes and establishing the minimum infectious dose of PEDV in a feed matrix are important components in strengthening virus prevention and control methods. In this study, an experiment was performed involving 30 crossbred, 10-d-old pigs that were used as a bioassay model for the minimum infectious dose of PEDV in feed. The PEDV was first diluted using tissue culture media to form 8 serial 10-fold dilutions. An aliquot of the original stock virus at 5.6 x 105tissue culture infectious dose/ml (TCID50/ml), each serial PEDV …
Evaluating Chemical Mitigation Of Porcine Epidemic Diarrhea Virus (Pedv) In Swine Feed And Ingredients, R. A. Cochrane, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, R. A. Hesse, Jianqiang Zhang, M. D. Tokach, J. Bai, C. K. Jones
Evaluating Chemical Mitigation Of Porcine Epidemic Diarrhea Virus (Pedv) In Swine Feed And Ingredients, R. A. Cochrane, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, R. A. Hesse, Jianqiang Zhang, M. D. Tokach, J. Bai, C. K. Jones
Kansas Agricultural Experiment Station Research Reports
Porcine Epidemic Diarrhea Virus (PEDV) is primarily transmitted by fecal-oral contamination. Research has confirmed swine feed or ingredients as potential vectors of transmission, so strategies are needed to mitigate PEDV in feed. The objective of this experiment was to evaluate the effectiveness of various chemical additives to prevent or mitigate post-processing PEDV contamination in swine feed and ingredients. Treatments were arranged in a 7 × 4 factorial with seven chemical treatments and four feed matrices. The chemical treatments included: negative control with no chemical addition, 0.3% commercial formaldehyde product, 1% sodium bisulfate, 1% sodium chlorate, 3% custom organic acid blend …
Evaluating The Effect Of Manufacturing Porcine Epidemic Diarrhea Virus (Pedv)-Contaminated Feed On Subsequent Feed Mill Environmental Surface Contamination, L. L. Schumacher, R. A. Cochrane, C. E. Evans, J. R. Kalivoda, J. C. Woodworth, C. R. Stark, C. K. Jones, Rodger G. Main, Jianqiang Zhang, S. S. Dritz, Phillip Charles Gauger
Evaluating The Effect Of Manufacturing Porcine Epidemic Diarrhea Virus (Pedv)-Contaminated Feed On Subsequent Feed Mill Environmental Surface Contamination, L. L. Schumacher, R. A. Cochrane, C. E. Evans, J. R. Kalivoda, J. C. Woodworth, C. R. Stark, C. K. Jones, Rodger G. Main, Jianqiang Zhang, S. S. Dritz, Phillip Charles Gauger
Kansas Agricultural Experiment Station Research Reports
This study aimed to utilize the only known pilot feed mill facility approved for pathogenic feed agent use in the United States to evaluate the effect of manufacturing Porcine Epidemic Diarrhea Virus (PEDV)-contaminated feed on subsequent feed mill environmental surface contamination. In this study, PEDV inoculated feed was manufactured and conveyed on equipment along with four subsequent batches of PEDV-free feed. Equipment and environmental surfaces were sampled using swabs and analyzed for the presence of PEDV RNA by PCR. The experiment was replicated three times with decontamination of the feed mill and all equipment between replications. Overall, environmental swabs indicated …
Utilizing Feed Sequencing To Decrease The Risk Of Porcine Epidemic Diarrhea Virus (Pedv) Cross-Contamination During Feed Manufacturing, L. L. Schumacher, R. A. Cochrane, J. C. Woodworth, C. R. Stark, C. K. Jones, Rodger G. Main, Jianqiang Zhang, Phillip Charles Gauger, S. S. Dritz, M. D. Tokach
Utilizing Feed Sequencing To Decrease The Risk Of Porcine Epidemic Diarrhea Virus (Pedv) Cross-Contamination During Feed Manufacturing, L. L. Schumacher, R. A. Cochrane, J. C. Woodworth, C. R. Stark, C. K. Jones, Rodger G. Main, Jianqiang Zhang, Phillip Charles Gauger, S. S. Dritz, M. D. Tokach
Kansas Agricultural Experiment Station Research Reports
Understanding key points of potential cross-contamination during the feed manufacturing process is important to developing efficacious methods to control or prevent transmission of pathogens into swine diets. In this study, an experiment was conducted involving 30 crossbred 10-d-old pigs that were used as a bioassay model for Porcine Epidemic Diarrhea Virus (PEDV) to determine the effects of feed batch sequencing on PEDV cross-contamination and subsequent infectivity. PEDV with a PCR cycle threshold value (Ct) of 11 was uniformly mixed into 4.5 kg of swine diet using a stainless steel bench top mixer validated for mixing efficiency. The inoculated feed was …