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Kansas Agricultural Experiment Station Research Reports
1998; Beef; Cytosine; Protein degradability; Microbial growth
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Development Of An In Vitro Procedure To Determine Ruminal Availability Of Protein, C.K. Schoenholz, James S. Drouillard, C.K. Armendariz, Evan C. Titgemeyer
Development Of An In Vitro Procedure To Determine Ruminal Availability Of Protein, C.K. Schoenholz, James S. Drouillard, C.K. Armendariz, Evan C. Titgemeyer
Kansas Agricultural Experiment Station Research Reports
A series of in vitro experiments was conducted to determine the ruminal availability of protein from grains. Procedures were based on assumptions that 1) ruminal availability of protein is first-limiting to microbial growth, 2) accumulation of microbial cells accurately predicts ruminal protein availability,3) cytosine can be used to accurately estimate microbial cell mass, and 4) cytosine is present in microorganisms but not in feeds. Cytosine content of in vitro cultures was measured by high performance liquid chromatography. Early experiments determined that adding 0.75 g soluble starch provided enough energy that culture growth depended on available protein. In the final experiment, …