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Beta-Hydroxybutyrate Alters The Mrna Cytokine Profile From Mouse Macrophages Challenged With Streptococcus Uberis, T. H. Swartz, L. K. Mamedova, B. J. Bradford
Beta-Hydroxybutyrate Alters The Mrna Cytokine Profile From Mouse Macrophages Challenged With Streptococcus Uberis, T. H. Swartz, L. K. Mamedova, B. J. Bradford
Kansas Agricultural Experiment Station Research Reports
The objective of this study was to determine if β-hydroxybutyrate (BHB) altered inflammatory responses in macrophages challenged with a common mastitis pathogen,Streptococcus uberis. Mouse macrophages (RAW 264.7 line) were cultured either in the presence or absence of BHB for 24 h, and then challenged or not withS. uberis. Relative transcript abundance of cell membrane receptors (TLR2 and GPR109a), cytokines (IL-1β, IL-10, TNFα, and TGFβ), and chemokines (CXCL2 and CCL5) were determined using quantitative real-time polymerase chain reaction (qPCR) and normalized against the geometric mean of HPRT and B2M.Streptococcus uberisactivated the macrophages, noted by greater …
Effects Of Dietary Supplementation Of Scutellaria Baicalensis Extract During Early Lactation On Milk Production Of Dairy Cattle, K. Olagaray, Michael Brouk, F. Robert, E. Dupuis, B. Bradford
Effects Of Dietary Supplementation Of Scutellaria Baicalensis Extract During Early Lactation On Milk Production Of Dairy Cattle, K. Olagaray, Michael Brouk, F. Robert, E. Dupuis, B. Bradford
Kansas Agricultural Experiment Station Research Reports
Multiparous Holstein cows (n = 122) were used in a randomized block design to determine the effect of short-term and long-term postpartum administration ofScutellaria baicalensisextract (SBE) on 305-day milk yield, 120-day milk component yield, and early lactation milk markers of inflammation and metabolic function. Treatments were (1) control, (2) short-term (5-day) administration of the SBE (SBE5), and (3) longterm (60-day) administration of the SBE (SBE60). Treatments were included in a treatment pellet that was identical to the control pellet in ingredient source and composition except for the extract, and both pellets were provided via an automated milking system. …