Life Sciences Commons^™

Sequencing An Inserted Concatemer In Caenorhabditis Elegans Strain Hlh-29::Gfp, Simon T. Anderson

Senior Honors Projects, 2020-current

Hairy/Enhancer of Split (HES) proteins and their homologs play vital roles in the development of animals, and in the regulation of cancer and other diseases. HLH-29 is an REF-1 family protein and a Caenorhabditis elegans homolog of human HES proteins. A transgenic animal model has been developed to facilitate analyses of this protein, containing an inserted concatemer of the green fluorescent protein reporter gene, regulated by the hlh-29 promoter, and a mutant rol-6 allele. Though the strain has been used previously, the location and structure of the insertion has been unknown. Sequencing of the whole hlh-29::gfp genome with high-molecular weight …

Narrative Review: Food Image Use For Machine Learnings’ Function In Dietary Assessment And Real Time Nutrition Feedback And Education, Jason Fee

Masters Theses, 2020-current

Technology has played a key role in advancing the health and agriculture sectors to improve obesity rates, diseasecontrol, food waste, and overall health disparities. However, these health and lifestyle determinants continue to plague theUnited States population. While new technologies have been and are currently being developed to address these concerns, they may not be practical for the general population. Utilizing machine learning advancement in food recognition using smartphone technology may be a means to improve the dietary component of nutrition assessments while providing valuable nutrition feedback. This narrative review was conducted to assess the current state of the literature on …

Pre-Pulse Inhibition In Mutated Mice: Studying Compromised Microglial Cells To Discover New Genetic Connections To Autism, Bailey R. Kramarik

Senior Honors Projects, 2020-current

Microglial cells “play a pivotal role in refining neural networks during early critical periods” (Gabriele & Gray, 2020, p. 1). A disturbance in the functioning of these microglial cells contribute to specific characteristics of some neurodevelopmental disorders- including autism spectrum disorder. In this study, we used a mouse model to study disruptions in cell activity, as understanding the biological and genetic background of autism spectrum disorder could help us provide better treatment and therapy options to those diagnosed.

The mutated mice in this experiment have microglial cells with “compromised fractalkine signaling” (Gabriele & Gray, 2020, p. 4-5). We studied multimodal …

The Effect Of Acute Caffeine Withdrawal On Exercise Performance In Habitual Caffeine Users, Timothy D. Griest

Masters Theses, 2020-current

Purpose: The purpose of this study was to investigate how exercise performance is impacted by acute withdrawal from caffeine (CAF) in habitual users and if CAF ingestion will reverse the negative impact of withdrawal or have a net positive benefit. Methods: Ten recreational cyclists (age 39.1 ± 14.9 y; VO_2max 54.2 ± 6.2 mL/kg/min) who were habitual CAF consumers (394 ± 146 mg/d) completed four trials, each consisting of peak isokinetic torque testing and a 10-km time trial (TT). On each trial day, subjects consumed either 1.5 mg/kg CAF to prevent withdrawal or placebo (PLA) 8 hours before their …

The Effect Of Creatine Supplementation On Body Composition And Total Body Water Measured By Multi-Frequency Bioelectrical Impedance, Emily A. Buck

Masters Theses, 2020-current

Background: Acute fluid ingestion causes an increase in estimated body fat percentage (BF%) measurements by single frequency (SF-BIA) and multi-frequency bioelectrical impedance (MF-BIA). However, it is unknown if MF-BIA accurately measures total body water (TBW) and BF% after chronic fluid retention. Creatine supplementation causes fluid retention, and resultant increases in TBW and body mass. Research is needed to determine if MF-BIA is capable of detecting fluid retention secondary to creatine supplementation. Methods: 13 male and 14 female subjects (18-22 y) completed one week of creatine monohydrate or maltodextrin supplementation at a dose of 0.3 g/kg body weight. Subjects completed pre-supplementation …

The Effect Of Dietary Nitrate Supplementation On Physical And Cognitive Performance During Load Carriage In Military Cadets, Nicholas C. Bordonie

Masters Theses, 2020-current

Purpose: The purpose of this study was to determine the effects of dietary nitrate supplementation on physical and cognitive performance during exercise conducted under heavy pack load in military cadets. Methods: Ten college-aged males (VO_2max 56.2 ± 3.7 ml/kg/min) consumed 140mL/d of concentrated beetroot juice (BRJ; containing 12.8 mmol of dietary nitrate) or a placebo (PL; flavor, color, energy, and texture-matched with no dietary nitrate) for six days preceding an exercise trial. The trial consisted of 45 min of constant-load exercise on a treadmill at 4.83 km/h and 1.5% grade, followed immediately by a 1.6-km time-trial completed at maximal …

The Impact Of Iaa And Tryptophan On Microcystis Bloom Community Dynamics, Hunter R. Baylous

Masters Theses, 2020-current

Microcystis aeruginosa is a freshwater cyanobacterial species that degrades freshwater and brackish ecosystems due to its capacity to form toxic cyanobacterial harmful algal blooms (cyanoHABs). There both abiotic and the biotic factors that exacerbate the presence of cyanoHABs. Current research is looking at the microbial interactions that occur between microbes and phytoplankton. Microbes interact with cyanobacteria in the phycosphere, where nutrients are exchanged between the two. Understanding the chemical currencies exchanged can help to show interactions that are beneficial for cyanoHAB formation A key player in the growth promotion of cyanoHABs is hypothesized to be auxins, which are synthesized by …

Pilfering Passerines? Inter-Class Competition: A New Direction In Foraging Ecology, Shannon Gillen

Masters Theses, 2020-current

Life in the epigeal layer below leaf litterfall often goes unexplored. Shrews (Soricidae, 1910) have foraged in this microhabitat for millions of years, carving out an existence in harsh habitats around the world, ranging from the tropics to subarctic boreal tundra. Two shrew species that inhabit the Shenandoah Valley of Virginia, the least shrew (Cryptotis parva) and northern short-tailed shrew (Blarina brevicauda), were the focus of this study. Shrews make epigeal foraging trails while they continually forage for food, and caching is a way to save this food for times of low and sporadic food availability. …

The Effect Of Intensity And Duration Of Verification Stages On The Efficacy Of Vo2max Testing, Andrew Foster

Masters Theses, 2020-current

Purpose: To determine if changing the length of the recovery stage influences the effectiveness, or ability to confirm maximal oxygen consumption (VO_2max), of the verification phase and if the initial intensity of the stage has an impact on its effectiveness. Methods: 27 subjects (20 males and 7 females) performed four separate VO_2max tests separated by at least 48 hours. For each initial graded exercise test, starting speed was 3.0 mph and increased by 0.5 mph every minute until 6.0 mph was reached. After this point, elevation was increased by 3.0% every minute until volitional exhaustion. VO_{2 …}

Does A Bout Of Acute Resistance Exercise Attenuate The Post-Prandial Lipemic Response To A High Fat Meal In 50-80 Year-Old Adults, Summer Simulcik

Masters Theses, 2020-current

Background: A single high-fat meal (HFM) is known to elevate blood lipid levels above what is normal, resulting in postprandial lipemia (PPL). Chronic hyperlipemia has been associated with increased risk for cardiovascular disease (CVD) and type-2 diabetes mellitus (T2DM) development. Current physical activity (PA) guidelines recommend at least one set of 8-12 repetitions of each exercise to attenuate this risk. Adults older than 50 (OF) years (y) are at an increased risk for cardiometabolic disease compared to younger adults (YA). Aerobic exercise (AE) performed in the pre-prandial period has been observed to attenuate PPL in both OF and YA. However, …

Urban Habitats Impact The Song And Reproductive Success Of A Migratory Bird, The Gray Catbird (Dumetella Carolinensis), Sasha Ewing

Masters Theses, 2020-current

Urban environments are relatively recent on an evolutionary timescale, and, as such, they create novel selection pressures that may influence fitness and mate choice. Many ecologically significant factors change with increasing levels of urbanization, such as increasing anthropogenic noise, increasing impervious surface, and decreasing forest cover. There is growing empirical evidence that anthropogenic noise affects features of birdsong and, separately, that avian nesting success varies across an urban gradient, but rarely do studies examine both – a necessary step to elucidate the evolutionary potential of these novel pressures. I investigated song features, body morphometrics, and reproductive success in male gray …

Assessing Red Spruce (Picea Rubens) Restoration Potential Under Current And Future Predicted Climate Change In Virginia, Christian Brown

Masters Theses, 2020-current

Global climate change threatens many species across the planet. High-elevation species, such as red spruce (Picea rubens), face significant and immediate threats from climate change. Red spruce has faced anthropogenic disturbances for over a century and is only recently beginning to regenerate across its range, making it an ideal restoration candidate. Ecological niche modeling has become a common method of identifying the suitable habitat of a species, providing vital information to land managers carrying out restoration efforts. In this study ecological niche models were used in a novel way, predicting distribution and habitat suitability separately to determine the …

Urbanization Drives Changes In Avian Communities, Which Are Reflected In The Song Of A Vocal Mimic, Jaclyn Tolchin

Masters Theses, 2020-current

Urban landscapes present problems for wildlife including highly modified habitat, anthropogenic noise pollution, and competition for suitable habitat. These novel selection pressures can filter species present in urban habitats or result in changes to behavior. Recent studies show that some bird species sing differently (louder, higher pitch, faster) in noisier, urban areas compared to quieter, more rural areas, and that anthropogenic noise alone can exclude species. I investigated how the level of urbanization affects 1) bird species composition, and 2) the assemblage of species that a songbird mimics from the local bird community. I studied gray catbirds, Dumetella carolinensis, across …

Identifying Key Stream Restoration Variables In An Agriculturally Impaired Chesapeake Bay Watershed, Julia Portmann

Masters Theses, 2020-current

The Chesapeake Bay watershed spans several states, supports diverse ecosystems, and is crucial to local economies. However, agricultural practices in this region impair water quality. The Smith Creek watershed, within the Shenandoah Valley, was designated a showcase watershed in 2010 by the United States Department of Agriculture to demonstrate the efficacy of implementing restoration projects. We sampled fifteen farms ranging from unrestored to thirty-six years since restoration. At each site, we conducted a kick-net survey for macroinvertebrates, measured canopy cover, algal density, substrate size, and bank height and angle. We identified macroinvertebrates to family and calculated the Chesapeake Basin-wide Index …

Trauma Healing With The Neurosequential Model Of Therapy And Bal-A-Vis-X, Becky Johnston

Educational Specialist, 2020-current

Stigma related to childhood trauma is shifting with the help of advancements in the understanding of neurobiology and interventions that are proving to be effective for healing. There are immense costs and consequences for survivors of childhood trauma and their loved ones that were not so long ago considered irrelevant and the notion that kids bounce back from adversity was previously popular in the psychological community (Perry & Szalavitz, 2017). The broad strokes of Dr. Bruce Perry’s clinical intervention model, The Neurosequential Model of Therapy (NMT) describes a trauma-sensitive, sequential approach to changing the stress response within mental health counseling. …