Life Sciences Commons^™

Quantification Of Neuronal Specific Responses To Depletion Of The Nascent Polypeptide-Associated Complex In Caenorhabditis Elegans, Harrison Hall

Senior Honors Projects, 2010-2019

Cells experiencing misfolded protein stress can become debilitated and die, consistent with this stress being linked to numerous diseases. When misfolded proteins accumulate in the endoplasmic reticulum (ER), the unfolded protein response (UPR) initiates mechanisms that resolve this stress or trigger apoptosis, dependent on the severity and/or duration of the stress. The nascent polypeptide-associated complex (NAC) is a heterodimeric chaperone that mediates proper protein folding and localization during translation; depletion of the NAC promotes misfolded protein stress in the ER, resulting in the initiation of the UPR in affected cells. The relationship between the NAC and the UPR is not …

The Effect Of An Acute Bout Of Exercise On Postprandial Metabolic Responses In Younger And Older Adults, Hannah Frick

Masters Theses, 2010-2019

Over 370,000 Americans, primarily between the ages of 60 and 79, die annually as a result of cardiovascular disease caused by physical inactivity and a diet that is calorically dense and high in fat. Current findings in young and middle-aged adults suggest that acute exercise with energy expenditure in excess of 500 kcals, may attenuate the deleterious health effects caused by consuming the typical American meal. Purpose:The purpose of this study was to examine the effects of acute exercise and aging on postprandial lipemia (PPL) and postprandial glycemia (PPG). We hypothesized that aerobic exercise performed prior to the consumption …

Effects Of A 12-Week Vigorous Interval Training Intervention On Pro-Inflammatory Markers In Post-Operative Bariatric And Obese Participants, Brittany Rood

Masters Theses, 2010-2019

Increase prevalence of cardiovascular and other chronic diseases linked to inflammation has led to research on pro-inflammatory cytokines, like tumor necrosis factor - alpha (TNF-α) and lipoprotein-associated phospholipase A₂ (Lp-PLA₂), in attempt to define the relationship between chronic disease, inflammation, and obesity. PURPOSE: Examine the effect of twelve weeks of vigorous intensity interval training on pro-inflammatory markers in post-operative bariatric and obese participants. METHODS: 9 post-operative bariatric individuals [1 man, 8 women; Body mass index (BMI) = 34.23 ± 2.54 kg/m²], 12 obese individuals [5 men, 7 women; BMI=35.77 ± 1.67 kg/m^{2 …}

Decrypting Female Attractivity In Garter Snakes, Holly Rucker

Senior Honors Projects, 2010-2019

Pheromones are utilized by many species as sexual signals driving mate choice, and pheromone production in vertebrates hinges on sex hormone action. Female red-sided garter snakes (Thamnophis sirtalis parietalis) produce a skin-based sex pheromone used by males for mate detection and selection. Estradiol is necessary for pheromone production, yet the specific mechanisms within the skin are unknown. Central to this is the metabolism of testosterone to estradiol via the enzyme aromatase. It is hypothesized that female garter snakes synthesize estradiol locally in the skin and maintain pheromone production via tissue-specific regulation of aromatase. Further, I hypothesize that female …

A Feasibility Study Of Industrial Hemp On Virginia Commercial Poultry Production, Aaron Sloss

Senior Honors Projects, 2010-2019

This project sought to characterize industrial hemp as a potential competitive agricultural commodity for the state of Virginia. Specifically, industrial hempseed was evaluated as a feedstock for the commercial broiler (meat-chickens) industry in Virginia.

Data were gathered to identify both the advantages and disadvantages of industrial hempseed as a nutritional resource for broilers. Key nutritional compositions were identified, quantified, and compared to several common feed ingredients, such as soybean, wheat, corn, and sorghum. Hempseed (whole seed) was found to have a considerably high amino acid content, protein, fiber, and metabolizable energy – making it an attractive alternative to conventional feedstuffs. …

Increasing Food Security: Developing And Testing A Nutrition Education Curriculum For A Mobile Food Pantry, Alexandra Lepecha, Kathleen M. Kraft

Senior Honors Projects, 2010-2019

Background: The Neighborhood Produce Market (NPM) is a food distribution model similar to a mobile food pantry. NPM stakeholders observed a lack of community engagement and familiarity with produce offered.

Project Description: The objective was to develop a nutrition education curriculum for NPM volunteers to better engage families and community members by providing food samples, recipes, and nutrition education. A stakeholder steering committee guided the needs assessment, curriculum development, volunteer training, and pilot test. The curriculum consisted of food safety, cultural considerations, and nutritional information for nine produce items and food tasting procedures and recipes. Seven student volunteers were trained …

Analyzing The Effect Of Apoptotic Mutations On The State Of The Nascent-Polypeptide Associated Complex In Caenorhabditis Elegans, Monica Gerber

Senior Honors Projects, 2010-2019

Cells experiencing misfolded protein stress can become debilitated and die, contributing to the onset of disease. The nascent polypeptide-associated complex (NAC) is a heterodimeric translational chaperone that protects against misfolded protein stress by mediating proper protein folding and localization during translation. Depletion of this complex results in misfolded protein accumulation in the endoplasmic reticulum (ER). To determine the importance of the NAC to proteostasis, we have previously depleted the complex in C.elegans via RNA interference and observed numerous dose-dependent effects, including apoptosis of neuronal cells and changes in gene expression of hypodermal cells. While we have observed these cell-specific responses …

Investigating The Role Of Integrin Beta 3 In Dendritic Arborization In The Supragranular Developing Cerebral Cortex, Zachary Logan Holley

Senior Honors Projects, 2010-2019

Integrin subunits have been implicated in axonal and dendritic outgrowth. In particular, a strong positive association has been found between mutations in integrin beta 3 (Itgb3) and autism spectrum disorder, but little is known about neuronal Itgb3 function in vivo. Many forms of autism spectrum disorder are thought to arise from dysfunctional dendritic arborization and synaptic pruning. Global knockout of Itgb3 in mice leads to autistic-like behaviors. Itgb3^-/- mice also have reduced callosal volume, a key neuroanatomical correlate of autism. Here, we test the hypothesis that Itgb3 is required for normal dendritic arborization in layer II/III pyramidal …

Detecting The Cold: Do Innexins Function In Cold Nociception?, Rachel Barborek

Senior Honors Projects, 2010-2019

Nociception is the perception of and response to harmful stimuli. Nociception is essential for minimizing tissue damage, but aberrant nociceptive pathways can result in chronic pain. Chronic pain in the U.S. is commonly managed with wide-acting opioids, and precisely defining the components of nociceptive pathways could uncover novel targets for pain therapies. I hypothesize that the vitally quick process of nociception would utilize electrical synapses because they transmit signals between neurons more quickly than chemical synapses do. This study, therefore, aims to uncover the potential role of the eight Drosophila melanogaster gap junction proteins, the Innexins, in cold nociception. Wild …

Examining The Effect Of Interstitial Space On Eastern Oysters (Crassostrea Virginica): Applications Of Photogrammetry And Three-Dimensional Modeling, Bailie Lavan

Masters Theses, 2010-2019

Global oyster populations have decreased by as much as 99% in the past century. Oysters are known ecosystem engineers, providing benthic habitat for macrofauna, linking benthic and pelagic food webs, improving water quality, and mitigating shoreline erosion. Restoration efforts are critical in re-establishing native oyster populations. In the Chesapeake Bay, where oyster loss is primarily due to severe over harvest, artificial substrates with geometric shapes are widely used in restoration efforts. However, natural oyster reefs form emergent shapes with a high degree of aggregation and many interstitial spaces (three-dimensional volumetric spaces between oysters within a reef). The lack of interstitial …

The Microcystis Microbiome: Interactions With Heterotrophic Bacteria In The Microcystis Phycosphere, Alexa Hoke

Masters Theses, 2010-2019

Harmful algal blooms are a growing problem globally in both freshwater and marine systems. Cyanobacterial harmful algal blooms (cHABs) can have numerous environmental and economic impacts. cHABs can reduce oxygen levels in the water column, ultimately leading to the death of larger organisms, and reduce light penetration, impacting aquatic vegetation. cHABs can also release toxins into the water and efforts to prevent toxins from entering drinking water can cost cities millions of dollars in added filtration. Microcystis is a freshwater cyanobacterium that is globally distributed. While many of the abiotic factors that can impact bloom formation are known, biotic factors, …

Expiratory Flow Limitation In Elite Youth Cyclists, Katherine R. Smith

Masters Theses, 2010-2019

Expiratory flow limitation (EFL) has been reported in pre-pubescent adolescents and elite, adult endurance athletes; however, the occurrence of EFL in elite adolescent endurance athletes has not been determined. Purpose: To determine incidence and severity of EFL in elite youth male cyclists of adolescent age. We hypothesized that elite, endurance trained youth will experience a higher prevalence and severity of EFL and dyspnea compared to an untrained control group. Pulmonary function will be similar pre- to post-exercise with bronchodilation occurring in both groups. Methods: 12 elite endurance- trained (ET) youth male cyclists (16.3 ± 1.0 years; 176.5 ± …

Influence Of Post-Exercise Nutrient Intake On Recovery And Subsequent Exercise Performance In Youth Cyclists, Andrew Law

Masters Theses, 2010-2019

Purpose: The purpose of this study was to determine if chocolate milk (CM) consumption after high-intensity cycling exercise affects post-exercise recovery and subsequent exercise performance in youth cyclists, compared to a carbohydrate-only (CHO) and a placebo (PL) beverage. Methods: Eight youth cyclists (15-18 y, VO_2peak = 61.8 ± 7.7 mL·kg^-1·min^-1) performed an exercise/recovery protocol consisting of 2 bouts of exercise, on 3 separate occasions, in a randomly counterbalanced crossover design. The first exercise bout (EX1) consisted of 30 min of constant-load cycling (40-60% W_max), and 60 min of high-intensity intervals (alternating 2 min …

Anti-Crispr Vs. Crispr: The Evolutionary Arms Race Between Microorganisms, Rachael M. St. Jacques

Masters Theses, 2010-2019

CRISPR arrays are a defense mechanism employed by bacteria against viral invaders. Cas proteins do the work in detecting, capturing, and integrating the viral DNA into the CRISPR array (Barrangou et al., 2007). Anti-CRISPR proteins are produced by phages, viruses that infect bacteria, to stop the bacterial host’s CRISPR-Cas complex from interrupting the phage life cycle (Bondy-Denomy, et al., 2015).

SEA-PHAGES is a course-based bacteriophage research network composed of 120 colleges and known at James Madison University as Viral Discovery. JMU uses the unsequenced Streptomyces griseus ATCC10137 as a host for bacteriophage discovery and propagation, and in this study we …

Does Acute Preprandial Exercise Modify Postprandial Inflammation After A High Fat Meal In Young And Older Adults?, William Wisseman

Masters Theses, 2010-2019

A single high-fat meal (HFM) can increase systemic inflammation (postprandial inflammation; PPI) and may be attenuated by recent exercise. However, the effect of preprandial exercise on PPI in older adults (OA) is not clear. Purpose: To determine if preprandial exercise attenuates PPI in YA and OA. Methods: 12 YA (23.3±3.9 years n= 5 M/7 F) and 12 OA (67.7±6 years, n= 8 M/4 F) completed two HFM challenges in a randomized order. During HFM alone, participants abstained from exercise 48 hours prior to the HFM session (except for the exercise session) and adhered to a 12 hour overnight …

How Successful High School Boys Soccer Coaches Perceive And Develop Cultural Competency: A Grounded Theory Approach, Lauren Jefferson

Masters Theses, 2010-2019

The purpose of this study was to examine how high school athletics coaches conceptualize the knowledge, values, and skills of cultural competence, with specific attention to learning processes and influences. In order to serve the increasingly diverse U.S. student population equitably and to the full holistic potential of extracurricular programming, high school coaches must develop a greater comfort with and capacity for exercising cultural competency. A qualitative approach using a grounded theory was applied. Seven coaches and one athletic director were recruited by purposive sampling. The research suggests a process-oriented, chronological model of how experienced coaches begin to work with …

Reproductive Character Displacement In Calopteryx Aequabilis And C. Maculata: Improving Species Recognition Through The Divergence Of Male Mating Preferences, Melissa Encinias

Masters Theses, 2010-2019

An ongoing evolutionary question is how co-occurring species maintain reproductive barriers when they are morphologically, behaviorally, and ecologically similar. Without geographic isolation, traits involved in species recognition may be under selection to enhance reproductive barriers. Exaggerated trait differences between species in sympatric populations may reflect selection to reduce misdirected mating between species, or reproductive character displacement. While this phenomenon is widely recognized as an important stage in the speciation process, there is little direct evidence of this process in nature. In two North American damselfly species, Calopteryx aequabilis and C. maculata, wing pigmentation is sexually dimorphic and also shows …

Assessing Populations Of Eastern Brook Trout (Salvelinus Fontinalis) Above And Below Waterfalls In Mountain Streams Of Virginia, Hannah Eisemann Macmillan

Masters Theses, 2010-2019

Anthropogenically driven factors, such as increasing temperature and sediment in valley streams, acidification of mountain streams, and the introduction of non-native trout, are restricting habitat suitable for healthy populations of eastern brook trout (Salvelinus fontinalis) throughout their native Appalachian range. Brook trout are important as predators of insects in mountain streams and as a favorite of anglers. It is crucial that remaining populations in sustainable habitats be identified and preserved. Waterfalls are geologic knickpoints preventing base-level lowering that create unique, stable landscapes above them, which may alleviate the temperature-productivity/acidity “habitat squeeze” for populations of brook trout and could …

Herpetological Assemblages In Tropical Dry Forests Of The Azuero Peninsula, Panama: An Evaluation Of Reforestation, Tyler Kovacs

Masters Theses, 2010-2019

Tropical dry forests are considered one of the most endangered tropical ecosystems making reforestation increasingly necessary to restore Panama’s unique ecoregion. The isolated dry ecoregion surrounding the Bay of Parita in Panama has a long history of deforestation and cattle grazing. Successful reforestation of this land is important to restore ecosystem health and biodiversity. In Panama, reforestation ranges from monocultures of exotic teak (Tectona grandis) to passive regeneration. Faunal recovery within these reforestation systems may vary due to different habitat characteristics. In this study, amphibian and reptile communities were compared in two types of reforestation systems and protected riparian forests …

In Vitro Propagation Of A Critically Endangered African Violets (Saintpaulia Rupicola B.L. Burtt), Thomas W. Lowry Iii

Masters Theses, 2010-2019

The in vitro propagation is a promising method for the production of plants in species that are endangered, commercially valuable, and/or otherwise difficult to propagate through traditional horticultural methods. Leaf and petiole explants of critically endangered African violets (Saintpaulia rupicola B.L. Burtt) were cultured on a chemically defined medium that was supplemented with six different concentrations of thidiazuron (TDZ). The concentrations were used for either an induction medium on which the explants remained throughout development, or an induction medium on which the explants remained for 10 days followed by a basal growth medium for the remainder of the 12 …

Urban Stream Restoration: An Evaluation Of Material Processing And Conveyance Channels, Madeline Berg

Masters Theses, 2010-2019

Stream restoration is gaining popularity in the Mid - Atlantic region to offset impacts from urbanization. Increased levels of impervious surfaces, decreased vegetation along banks, and changes in water flow patterns make urban stream ecosystems flashy and result in high erosion rates, increasing the amount of nutrients entering the Chesapeake Bay. Different restoration practices can play a large role in the amount of nutrients and organic matter leaving a stream and the amount of habitat that is present in-stream.

Due to the recent interest in stream restoration as a tool to help the health of the Chesapeake Bay, this study …

Forest Structure Of Long-Term Conserved Areas Utilizing Different Strategies On A Continental, Glacial Moraine Formed Island, Christopher Coggin

Masters Theses, 2010-2019

Understanding patterns of forest succession can help advise management plans within New England nature preserves. This study took place on Block Island, 13 miles off the coast of Rhode Island. The island has greater than 200+ years of farming practices. After 1960, conservation groups began reforesting the island using different strategies, such as actively planting with native and exotic tree species, mowing, and preventing further development. In 2018, woody vegetation was inventoried along transects within four reforested sites. Sites were characterized as the following: actively planted with exotic and native tree species and mowed (AP-M), actively planted with exotic and …

Impact Of One Night Sleep Restriction On Autonomic Function And Heart Rate Variability In Recreational Cyclists, Amanda J. Becker

Senior Honors Projects, 2010-2019

Introduction: Sleep quality has been shown to influence autonomic function. The autonomic nervous system (ANS) responds to stressors by regulating key functions such as heart rate. Autonomic function can be monitored via heart rate variability (HRV). Athletes are prone to poor sleep quality due to psychological and physical stresses of training and competing. Our objective was to investigate the function of the ANS at rest and during exercise following a night of normal sleep (SLP+) and a night of restricted sleep (SLP-).

Methods: Subjects (n=9) completed a familiarization trial and two identical experimental trials under different sleep conditions: SLP- (3-hour …

The One Health Initiative: The Intersection Of Human, Animal, And Environmental Health, Mary Evelyn Pearsall

Senior Honors Projects, 2010-2019

The One Health concept is a global strategy for strengthening interdisciplinary collaborations among health care for humans, animals, and the environment. The purpose of this honors capstone project is to study the One Health Initiative and create a comprehensive thesis on what the initiative is and how it is relevant to different sectors of health. An overview of prevalent zoonotic diseases will be provided including how they are transmitted and their global relevance. Case studies on zoonotic diseases will be used in which the effectivity of a One Health approach can be analyzed. In addition, relevant sections on One Health …

Structure/Function Analysis Of Fega And Fhua In Bradyrhizobium Sp., Alexander James Herd

Senior Honors Projects, 2010-2019

Bradyrhizobium japonicum is a Gram-negative soil bacterium commonly known for its agriculturally significant mutualistic relationship with soybean. In this symbiosis, the bacteria and plant undergo complex molecular signaling characterized by sent and received signals resulting in the formation of infection threads and root nodules. This research aimed to compare two related bacterial outer membrane proteins, FegA and FhuA, associated with the molecular signaling between the bacteria and plant. Previous work has led to the hypothesis that the N-terminal domain (NTD) of FegA in B. japonicum is needed for a functional symbiosis to occur. Recombinant bacterial strains expressing altered FegA proteins …

Assessing The Prevalence Of Multidrug Resistant Salmonella Enterica From Stream Sediment And Poultry Litter In The Shenandoah Valley Of Virginia, Noah Greenman

Masters Theses, 2010-2019

Multidrug resistant Salmonella enterica present in stream sediment and poultry litter represent a critical health concern. A small number of S. enterica serotypes are responsible for most lab-confirmed infections in the US each year. To assess the prevalence of these significant strains, we isolated 88 S. enterica from stream sediment and poultry litter. Sequence data for all isolates were generated using an Illumina® sequencing platform, with long-reads for some isolates from the Oxford Nanopore MinION™ used in a hybrid genome assembly approach. Isolates were typed according to their serotype and multi-locus sequence type using SeqSero/SISTR and Enterobase respectively. Antibiotic resistance …