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Screening For Volatile Fatty Acids In Agricultural Air Using Solid Phase Microextraction And Gas Chromatography – Mass Spectrometry, Jarett P. Spinhirne, Jacek A. Koziel, David B. Parker, Darren L. Williams, N. Andy Cole, John M. Sweeten
Screening For Volatile Fatty Acids In Agricultural Air Using Solid Phase Microextraction And Gas Chromatography – Mass Spectrometry, Jarett P. Spinhirne, Jacek A. Koziel, David B. Parker, Darren L. Williams, N. Andy Cole, John M. Sweeten
Jacek A. Koziel
Volatile fatty acids (VFAs) are a major component of odorous gases associated with agricultural sources. Because of typically low VFA air concentrations, conventional air sampling methods including sorbent tubes and vacuum canisters are often not sensitive enough to detect them. Solid phase microextraction (SPME) was used in this research because it is very sensitive, reusable, fast, and combines sampling and sample preparation, allowing for rapid detection of low concentrations of organics in air. This research focused on 7 VFAs: acetic, propionic, isobutyric, butyric, isovaleric, valeric, and hexanoic acids. Field experiments were conducted to test this method for air sampling of …
Noninvasive Sampling And Analysis Of Bovine Breath Using Solid-Phase Microextraction And Gas Chromatography-Mass Spectrometry: A Feasibility Study, Jacek A. Koziel, Jarett P. Spinhirne, Norbert K. Chirase
Noninvasive Sampling And Analysis Of Bovine Breath Using Solid-Phase Microextraction And Gas Chromatography-Mass Spectrometry: A Feasibility Study, Jacek A. Koziel, Jarett P. Spinhirne, Norbert K. Chirase
Jacek A. Koziel
An experiment was conducted to examine the feasibility of detecting unique volatile organic biomarkers exhaled by healthy and morbid heifer calves on-site using noninvasive sampling with solid phase microextraction (SPME) and analysis with gas chromatography-mass spectrometry (GC-MS). Two heifers were selected on the bases of health status and were sampled from 5 to 15 min with a special facemask sampling system using DVB/Carboxen/PDMS 50/30 µ m and PDMS 100 µ m SPME fibers. The samples were sealed and transported to the laboratory and analyzed with ion trap GC-MS. Acetone, methyl ethyl ketone, toluene, pentadecane, and nonanal were detected in cattle …