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Stimulation Of Pheromone Biosynthesis In The Moth Helicoverpa Zea: Action Of A Brain Hormone On Pheromone Glands Involves Ca2+ And Camp As Second Messengers, Russell A. Jurenka, Emmanuelle Jacquin, Wendell L. Roelofs
Stimulation Of Pheromone Biosynthesis In The Moth Helicoverpa Zea: Action Of A Brain Hormone On Pheromone Glands Involves Ca2+ And Camp As Second Messengers, Russell A. Jurenka, Emmanuelle Jacquin, Wendell L. Roelofs
Russell A. Jurenka
Isolated abdomen and pheromone gland bioassays were utilized to determine the physiological action of the pheromone-biosynthesis-activating neuropeptide (PBAN) in the corn earworm moth Helicoverpa (= Heliothis) zea. An isolated pheromone gland bioassay showed that synthetic PBAN was active at 0.02 pmol, with maximal activity occurring at 0.5 pmol and 60 min of incubation. Second-messenger studies demonstrated that extracellular Ca2+ is necessary for PBAN activity on isolated pheromone glands. The Ca2+ ionophore A23187 stimulated pheromone biosynthesis alone, whereas the Ca2+ channel blockers La3+ and Mn2+ inhibited PBAN activity. However, the organic Ca2+ channel blockers verapamil and nifedipine did not inhibit PBAN …