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Full-Text Articles in Life Sciences
Kinetic Characterization Of Listeria Monocytogenes 2-C- Methyl-D-Erythritol 4-Phosphate Cytidylyltransferase ( Cms ) Enzymes Using High Performance Liquid Chromatography ( Hplc ), Mark Oblazny
Theses and Dissertations
Infectious diseases, with increasing prevalence of antibiotic resistant bacteria, coupled with the declining rate in discovery of antimicrobial agents, impose one of the most significant threats to human health. Here we identify 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (CMS) as a valid target for antibiotic development which is an enzyme in pathogenic organisms that leads to the biosynthesis of isoprene precursor molecules. Isoprene molecules are one of nature’s most common building blocks that are vital to many biological metabolic processes and are synthesized via the mevalonic acid dependent (MVA), or methylerythritol phosphate (MEP) pathway. Vertebrates utilize the MVA pathway, while many pathogenic bacteria …
Î?- Lactam-Induced Changes In Cell Wall Composition And Autolytic Properties Of Daptomycin Resistant Staphylococcus Aureus, Michael Krzyskowski
Î?- Lactam-Induced Changes In Cell Wall Composition And Autolytic Properties Of Daptomycin Resistant Staphylococcus Aureus, Michael Krzyskowski
Theses and Dissertations
Staphylococcus aureus is a major human pathogen that is well known for its ability to develop resistance to antimicrobial therapies. Recent studies have identified a combination of β-lactam antibiotic and daptomycin (DAP) to be highly effective at killing single and multi-drug-resistant S. aureus. Of particular interest is this drug combinationâ??s efficiency with which it kills strains classified as DAP-resistant methicillin-resistant Staphylococcus aureus (DAP-R MRSA). The goals of the present study were two-fold: to provide a physiological profile of a DAP-R S. aureus strain undergoing beta-lactam treatment by observing changes in autolytic rate, susceptibility to lytic enzymes, levels of peptidoglycan O-acetylation, …