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Eccentric Contractions Disrupt Fkbp12 Content In Mouse Skeletal Muscle, Cory W. Baumann, Russell George Rogers Iii, Nidhi Gahlot, Chris Ingalls
Eccentric Contractions Disrupt Fkbp12 Content In Mouse Skeletal Muscle, Cory W. Baumann, Russell George Rogers Iii, Nidhi Gahlot, Chris Ingalls
Biology Faculty Publications
Strength deficits associated with eccentric contraction-induced muscle injury stem, in part, from impaired voltage-gated sarcoplasmic reticulum (SR) Ca2+ release. FKBP12 is a 12-kD immunophilin known to bind to the SR Ca2+ release channel (ryanodine receptor, RyR1) and plays an important role in excitation-contraction coupling. To assess the effects of eccentric contractions on FKBP12 content, we measured anterior crural muscle (tibialis anterior [TA], extensor digitorum longus [EDL], extensor hallucis longus muscles) strength and FKBP12 content in pellet and supernatant fractions after centrifugation via immunoblotting from mice before and after a single bout of either 150 eccentric or concentric contractions. There were …