Life Sciences Commons^™

Rickettsia Felis In Cat Fleas, Ctenocephalides Felis Parasitizing Opossums, San Bernardino County, California, Kyle F. Abramowicz, J. W. Wekesa, C. N. Nwadike, Maria L. Zambrano, Sandor E. Karpathy, D. Cecil, J. Burns, R. Hu, Marina E. Eremeeva

Environmental Health Sciences Faculty Publications

Los Angeles and Orange Counties are known endemic areas for murine typhus in California; however, no recent reports of flea-borne rickettsioses are known from adjacent San Bernardino County. Sixty-five opossums (Didelphis virginiana) were trapped in the suburban residential and industrial zones of the southwestern part of San Bernardino County in 2007. Sixty out of 65 opossums were infested with fleas, primarily cat fleas, Ctenocephalides felis (Bouché, 1835). The flea minimum infection rate with Rickettsia felis was 13.3% in pooled samples and the prevalence was 23.7% in single fleas, with two gltA genotypes detected. In spite of historic records of murine …

Expression Of Fused In Sarcoma Mutations In Mice Recapitulates The Neuropathology Of Fus Proteinopathies And Provides Insight Into Disease Pathogenesis, Christophe Verbeeck, Mariely Dejesus-Hernandez, Carolina Ceballos-Diaz, Jannet Kocerha, Todd Golde, Pritam Das, Rosa Rademakers, Dennis W. Dickson, Thomas Kukar

Jannet Kocerha

Background: Mutations in the gene encoding the RNA-binding protein fused in sarcoma (FUS) can cause familial and sporadic amyotrophic lateral sclerosis (ALS) and rarely frontotemproal dementia (FTD). FUS accumulates in neuronal cytoplasmic inclusions (NCIs) in ALS patients with FUS mutations. FUS is also a major pathologic marker for a group of less common forms of frontotemporal lobar degeneration (FTLD), which includes atypical FTLD with ubiquitinated inclusions (aFTLD-U), neuronal intermediate filament inclusion disease (NIFID) and basophilic inclusion body disease (BIBD). These diseases are now called FUS proteinopathies, because they share this disease marker. It is unknown how FUS mutations cause disease …

The Effects Of Locomotor Posture On Kinematics, Performance And Behavior During Obstacle Negotiation In Lizards, Jessica Self

Electronic Theses and Dissertations

The ability to efficiently move over uneven terrain is critical for most terrestrial animals. Bipedal running is common in lizard species, however the biological advantage of a bipedal running posture remains uncertain. I examined the hypothesis that a bipedal posture is advantageous when crossing obstacles. Particularly, I determined whether kinematic adjustments differ among four focal species with contrasting body forms and ecology. I also examined how sprint speed changed when crossing obstacles with a quadrupedal versus a bipedal posture. I quantified kinematics from high-speed video (300 frames/second) of lizards running down a 3m runway both with and without the presence …

Predation Risk And Colony Structure In The Pea Aphid, Acyrthosiphon Pisum, Carl Nicolas Keiser

Electronic Theses and Dissertations

Many organisms live in transient or permanent aggregations to reduce individual predation risk. Hamilton's "Selfish Herd" theory states that an individual should assume a central position within a group to decrease individual predation risk relative to that of its neighbors (i.e., individuals should be selfish). This theory, however, cannot predict the spatial distribution of individuals within clonal aggregations, that is, when individuals are genetically identical (the "evolutionary self"). As aphids (small, herbivorous insects) are parthenogenetic, emit alarm signals, and have high levels of phenotypic plasticity to cope with environmental stressors like predation risk, they are a model organism for investigating …

Role Of Provisions And Season On Foraging And Female Dominance Behavior In Ring-Tailed Lemurs (Lemur Catta) On St. Catherine's Island, Timothy Moore

Electronic Theses and Dissertations

Madagascar is undergoing habitat destruction and degradation that is threatening its unique species, including ring-tailed lemurs. Ring-tailed lemurs (Lemur catta) live in matriarchal groups composed of a dominant female, adult males, subadult males and females, and young. Due to their dominance, it has been suggested that female ringtailed lemurs have access to the highest quality resources and can monopolize food patches. Lemurs on St. Catherine's are free ranging and have unlimited access to natural food sources, but they are also provisioned with supplementary food to ensure a balanced diet. My study aimed to determine the impact of provisions on female …

Patterns Of Chemosensory Behavior In A Closed Population Of Wild African Elephants (Loxodonta Africana), Russell W. Blogg

Electronic Theses and Dissertations

Chemosensory behaviors are used by many mammalian species to assess chemical signals in the environment. These chemical signals may contain important information about reproductive state, identity, status, or location of conspecifics. Elephants are a long-lived species and males reproduce at a much later age than females, which provides a protracted developmental period for males. This study examined chemosensory behaviors in a population of African elephants living in Addo Elephant National Park, South Africa and demonstrated that patterns emerge as elephants develop and approach sexual maturity. Older pubescent males (15-19 year olds) performed more chemosensory behaviors than younger pubescent males (10-14 …

Spatial And Temporal Immune Response In House Flies In Response To Ingestion Of Bacillus Cereus And Eschericha Coli 0157-H7, Adam Fleming

Electronic Theses and Dissertations

House flies (Musca domestica L.) feed and breed on septic substrates, putting them in direct contact with a multitude of disease causing agents and can act as a bridge for those agents to humans. The house fly has previously been shown to carry many different species of bacteria that are pathogenic. Escherichia coli O157:H7 is a pathogenic enterohemorrhagic serotype of E. coli that can be vectored by the house fly. Bacillus cereus is a foodborne pathogen that has also been isolated from the house fly in previous studies. To examine vector potential for these pathogens, house flies were fed green …

Bird Use Of Cumberland Island's Freshwater Wetlands, Lisa Dlugolecki

Electronic Theses and Dissertations

Cumberland Island is the southernmost barrier island off the coast of Georgia. Its freshwater wetlands are an important, rare habitat to have on a barrier island surrounded by saltmarsh and ocean. Many species of birds require freshwater wetlands as feeding, roosting and nesting grounds. However, the freshwater wetlands on Cumberland Island have been impacted by humans for centuries causing birds to abandon their historic nesting ground. Known land use histories of Cumberland Island's freshwater wetlands were gathered to try and determine how the wetlands changed over time Wetlands were analyzed for presence of wetland-dependent birds and recorded. Thirty-six species of …

Endurance Tests Are The Most Reliable Core Stability Related Measurements, Andy Waldhelm, Li Li

Li Li

Purpose: To determine the intra-tester reliability of clinical measurements that assess five components related to core stability: strength, endurance, flexibility, motor control, and function.
Methods: Participants were 15 college-aged males who had not suffered any orthopedic injury in the past year. Core strength measurements included eight isometric tests and a sit-up test. The four core endurance tests were the trunk flexor test, trunk extensor test, and bilateral side bridge tests. Flexibility tests included the sit-and-reach test and active range of the trunk and hip joint motions. Proprioception via passive reposition tests of the hips and a single limb balance test …

Endurance Tests Are The Most Reliable Core Stability Related Measurements, Andy Waldhelm, Li Li

Department of Health Sciences and Kinesiology Faculty Publications

Purpose: To determine the intra-tester reliability of clinical measurements that assess five components related to core stability: strength, endurance, flexibility, motor control, and function. Methods: Participants were 15 college-aged males who had not suffered any orthopedic injury in the past year. Core strength measurements included eight isometric tests and a sit-up test. The four core endurance tests were the trunk flexor test, trunk extensor test, and bilateral side bridge tests. Flexibility tests included the sit-and-reach test and active range of the trunk and hip joint motions. Proprioception via passive reposition tests of the hips and a single limb balance test …

Rickettsia Felis In Ctenocephalides Felis From Guatemala And Costa Rica, Adriana Troyo, Danilo Alvarez, Lizeth Taylor, Gabriela Abdalla, Olger Calderon-Arguedas, Maria L. Zambrano, Gregory A. Dasch, Kim A. Lindblade, Laya Hun, Marina Eremeeva, Alejandra Estevez

Environmental Health Sciences Faculty Publications

Rickettsia felis is an emerging human pathogen associated primarily with the cat flea Ctenocephalides felis. In this study, we investigated the presence of Rickettsia felis in C. felis from Guatemala and Costa Rica. Ctenocephalides felis were collected directly from dogs and cats, and analyzed by polymerase chain reaction for Rickettsia-specific fragments of 17-kDa protein, OmpA, and citrate synthase genes. Rickettsia DNA was detected in 64% (55 of 86) and 58% (47 of 81) of flea pools in Guatemala and Costa Rica, respectively. Sequencing of gltA fragments identified R. felis genotype URRWXCal2 in samples from both countries, and genotype Rf2125 in …

The Path To Microrna Therapeutics In Psychiatric And Neurodegenerative Disorders, Anthony W. S. Chan, Jannet Kocerha

Jannet Kocerha

The microRNA (miRNA) class of non-coding RNAs exhibit a diverse range of regulatory roles in neuronal functions that are conserved from lower vertebrates to primates. Disruption of miRNA expression has compellingly been linked to pathogenesis in neuropsychiatric and neurodegenerative disorders, such as schizophrenia, Alzheimer’s disease, and autism. The list of transcript targets governed by a single miRNA provide a molecular paradigm applicable for therapeutic intervention. Indeed, reports have shown that specific manipulation of a miRNA in cell or animal models can significantly alter phenotypes linked with neurological disease. Here, we review how a diverse range of biological systems, including Drosophila, …

Muscular Activity Characteristics Associated With Preparation For Gait Transition, Li Li, Lorna L. Ogden

Department of Health Sciences and Kinesiology Faculty Publications

Purpose: The purpose of this study was to investigate lower extremity neuromuscular activity patterns during gait transitions with continuously changing locomotion speeds.

Methods: Muscular activities related to gait transitions (walk to run and run to walk) induced by changing treadmill speed were compared to muscular activities during walk and run at constant speeds. All transition and constant speed conditions were conducted in similar speed range. Surface electromyographic activities of gluteus maximus (GM), rectus femoris (RF), vastus lateralis (VL), biceps femoris long head (BFL), tibialis anterior (TA), gastrocnemius (GA), and soleus (SL) were collected and analyzed. The influences of speed and …

Muscular Activity Characteristics Associated With Preparation For Gait Transition, Li Li, Lorna L. Ogden

Li Li

Purpose: The purpose of this study was to investigate lower extremity neuromuscular activity patterns during gait transitions with continuously changing locomotion speeds.
Methods: Muscular activities related to gait transitions (walk to run and run to walk) induced by changing treadmill speed were compared to muscular activities during walk and run at constant speeds. All transition and constant speed conditions were conducted in similar speed range. Surface electromyographic activities of gluteus maximus (GM), rectus femoris (RF), vastus lateralis (VL), biceps femoris long head (BFL), tibialis anterior (TA), gastrocnemius (GA), and soleus (SL) were collected and analyzed. The influences of speed and …

People Can Live Longer By Having More Physical Activity, Li Li

Department of Health Sciences and Kinesiology Faculty Publications

We all know being physically active is good for you. But do we know how good? People can live up to 3 years longer, even with as little as 15 min of physical activity a day, according to last October's report by Wen and his colleagues¹at the China Medical University in Taiwan, China.

Many researchers in the field of sport and health sciences know being physically active can bring many benefits to one's life. But this message has sometimes been disseminated using a negative tone. So much so, Bortz² of California, USA, has coined the word “inactivity” …

Characterization Of Aquaporin 4 Protein Expression And Localization In Tissues Of The Dogfish (Squalus Acanthias), Christopher P. Cutler, Sheena Harmon, Jonathan Walsh, Kia Burch

Department of Biology Faculty Publications

The role of aquaporin water channels such as aquaporin 4 (Aqp4) in elasmobranchs such as the dogfish Squalus acanthias is completely unknown. This investigation set out to determine the expression and cellular and sub-cellular localization of Aqp4 protein in dogfish tissues. Two polyclonal antibodies were generated (AQP4/1 and AQP4/2) and these showed somewhat different characteristics in Western blotting and immunohistochemistry. Western blots using the AQP4/1 antibody showed two bands (35.5 and 49.5 kDa) in most tissues in a similar fashion to mammals. Liver had an additional band of 57 kDa and rectal gland two further faint bands of 37.5 and …

Aquaporin 4 Is A Ubiquitously Expressed Isoform In The Dogfish (Squalus Acanthias) Shark, Christopher P. Cutler, Bryce Maciver, Gordon Cramb, Mark L. Zeidel

Department of Biology Faculty Publications

The dogfish ortholog of aquaporin 4 (AQP4) was amplified from cDNA using degenerate PCR followed by cloning and sequencing. The complete coding region was then obtained using 5′ and 3′ RACE techniques. Alignment of the sequence with AQP4 amino acid sequences from other species showed that dogfish AQP4 has high levels (up to 65.3%) of homology with higher vertebrate sequences but lower levels of homology to Agnathan (38.2%) or teleost (57.5%) fish sequences. Northern blotting indicated that the dogfish mRNA was approximately 3.2 kb and was highly expressed in the rectal gland (a shark fluid secretory organ). Semi-quantitative PCR further …

Calcium Montmorillonite Clay Reduces Urinary Biomarkers Of Fumonisin B₁ Exposure In Rats And Humans, Abraham Robinson, Natalie M. Johnson, A. Strey, John F. Taylor, Alicia Marroquin-Cardona, N. J. Mitchell, Evans Afriyie-Gyawu, Nii-Ayi Ankrah, Jonathan H. Williams, Jia-Sheng Wang, Pauline E. Jolly, R. J. Nachman, Timothy D. Phillips

Environmental Health Sciences Faculty Publications

Fumonisin B1 (FB1) is often a co-contaminant with aflatoxin (AF) in grains and may enhance AF’s carcinogenicity by acting as a cancer promoter. Calcium montmorillonite (i.e. NovaSil, NS) is a possible dietary intervention to help decrease chronic aflatoxin exposure where populations are at risk. Previous studies show that an oral dose of NS clay was able to reduce AF exposure in a Ghanaian population. In vitro analyses from our laboratory indicated that FB1 (like aflatoxin) could also be sorbed onto the surfaces of NS. Hence, our objectives were to evaluate the efficacy of NS clay to reduce urinary FB1 in …

People Can Live Longer By Having More Physical Activity, Li Li

Li Li

We all know being physically active is good for you. But do we know how good? People can live up to 3 years longer, even with as little as 15 min of physical activity a day, according to last October's report by Wen and his colleagues1 at the China Medical University in Taiwan, China.
Many researchers in the field of sport and health sciences know being physically active can bring many benefits to one's life. But this message has sometimes been disseminated using a negative tone. So much so, Bortz2 of California, USA, has coined the word “inactivity” …

Comparative Analysis Of Microsatellite And Mitochondrial Genetic Variation In Ixodes Scapularis, Cynthia Tak Wan Chan

Electronic Theses and Dissertations

Ixodes scapularis, the black legged tick, is a species endemic to North America with a range including most of the eastern-half of the United States and portions of Canada and Mexico. The tick is an important vector of diseases transmitted to humans and animals. Since its first description in 1821, the taxonomy of the species has been controversial. Biological differences have been identified in the northern and southern populations, yet no consensus exists on population structure and the causes of this disparity. Earlier molecular studies utilizing nuclear and mitochondrial genetic markers have revealed the occurrence of two distinct lineages: …

Botanical Garden News

Botanical Garden News

No abstract provided.

Frankenfoods: Are We Bioengineering Our Children And What Can Curriculum Do?, Patra Rickman

Electronic Theses and Dissertations

In this dissertation I explore the effect of biotechnology and food production on children’s physical and mental health. What we eat affects our well-being and unknowingly, we have little control of what exactly we are consuming. Children have even less control and feel the effects of additives and chemicals more than adults. Many of the effects of bioengineered foods are unknown at this time. My dissertation will be a theoretical exploration of biotechnology in food using a bioethical critical perspective. I use my personal experience and research to examine the effects of processed and genetically engineered foods on people, especially …

Kinematic Analysis Of Four Plyometric Push-Up Variations, Laura H. Moore, Michael J. Tankovich, Bryan L. Riemann, George J. Davies

Department of Health Sciences and Kinesiology Faculty Publications

Plyometric research in the upper extremity is limited, with the effects of open-chain plyometric exercises being studied most. Kinematic and ground reaction force data concerning closed-chain upper extremity plyometrics has yet to be examined. Twenty-one recreationally active male subjects performed four variations of plyometric push-ups in a counterbalanced order. These included box drop push-ups from 3.8 cm, 7.6 cm, 11.4 cm heights, and clap push-ups. Kinematics of the trunk, dominant extremity and both hands were collected to examine peak flight, elbow flexion at ground contact, elbow displacement, and hand separation. Additionally peak vertical ground reaction force was measured under the …

Development Of High-Yielding Sweetgum Plantation Systems For Bioenergy Production In The Southeastern United States, Donald J. Kaczmarek, Brian C. Wachelka, Jeff Wright, Victor C. Steel, Doug P. Aubrey, David R. Coyle, Mark D. Coleman

Department of Biology Faculty Presentations

No abstract provided.

Evaluating Relationships Between Mercury Concentrations In Air And In Spanish Moss (Tillandsia Usneoides L.), Kathryn T. Sutton

Electronic Theses and Dissertations

Mercury is a potent neurotoxin that is transported globally in vapor form. A major source of mercury contamination to soil, water, and biota is atmospheric deposition. Therefore, comprehensive monitoring of atmospheric concentrations is important. Limitations of conventional atmospheric measurement techniques include high cost and lack of temporal or spatial integration. Bioindicators, however, may serve as an integrative tool to add to conventional mercury measurement techniques. Spanish moss (Tillandsia usneoides L.) is a potential bioindicator of atmospheric mercury concentration in the southeastern United States because it is an abundant epiphyte that absorbs and accumulates atmospheric pollutants. A study was conducted in …