Life Sciences Commons^™

The Biobehavioral Effects Of Embryonic Exposure To Neural Inflammation And Oxidative Stress In Zebrafish, Dalton J. Anderson

Honors College Theses

The purpose of this research is to improve understanding of the neurodevelopmental effects of embryonic exposure to elevated inflammation and oxidative stress induced by the antipyretic drug acetaminophen (APAP). Our study was the first to examine the interactive effects of APAP and inflammation in zebrafish embryos and how the treatments affect brain development and larval behavior. Experimental groups of zebrafish larvae were exposed to lipopolysaccharide (LPS) to induce inflammation, APAP, or LPS + APAP and larval behavior was analyzed using Ethovision automated behavioral tracking software. We also measured changes in whole-brain Glycogen Synthase Kinase 3 Beta (GSK3B) and GSK3B phosphorylation, …

The Effects Of Cucurbitacin B And Silmitasertib On Metastasis And Itga6 Using The Zebrafish Tumor-Xenograft, Alexandra Griffis

Electronic Theses and Dissertations

Ninety percent of cancer deaths are resultant from the metastasis of cancer cells. When cancer cells translocate through blood vessels or the lymphatic system, they may form tumors outside of their primary site. The processes of metastasis can begin quickly; after onset, metastasis is unforgiving, as it does not participate with the body’s physiological systems in an orderly way. In the past, our lab produced results indicating that a cell adhesion molecule, Integrin Alpha-6, may contribute to cancer cells' ability to metastasize. Integrins mediate interactions between the cell and the Extracellular Matrix (ECM), regulating cell attachment and cell migration. With …

Molecular Analysis Of Mchm Toxicity In Zebrafish, Cassandra Pelton

Honors College Theses

In early 2014 Freedom Industries, located in Charleston, WV, leaked approximately 10,000 gallons of 4-methylcyclohexane methanol into the Elk River. This river serviced approximately 300,000 people as a source of municipal water. Its effects on the people and surrounding wildlife is largely unknown.

Chemicals in surface runoff have the potential to induce aquatic animal trait changes, such as altered movement and feeding, that can ultimately alter predator-prey dynamics in aquatic systems. However, chemicals with high potential to enter aquatic systems, like 4-methylcyclohexane methanol (MCHM) used to clean coal, were tested using only short-term, single-species studies prior to use. Data from …

The Influence Of Autism Linked Gene Topoisomerase 3b (Top3b) On Neural Development In Zebrafish, Sydney Doolittle

Honors College Theses

Autism Spectrum Disorder is a class of developmental disabilities characterized by a spectrum of social, communication, and behavioral impairments in affected individuals. Studies have shown these defects stem from abnormal brain development during critical periods during early development. The underlying genetic cause of these impairments is not well understood but is believed to be a combination of a complex pairing of genetic and environmental factors. One of the genetic factors that has been recognized to influence the phenotypic symptoms of ASD is the enzyme topoisomerase 3β (top3β.) Topoisomerases are responsible for the prevention of supercoiling during DNA replication. Top3β is …

Using Zebrafish (Danio Rerio) To Evaluate The Use Of Food Waste Based Feed In Sustainable Small-Scale Aquaculture, Caitlin May

Electronic Theses and Dissertations

Food waste is rich in nutrients and a valuable resource, but currently its primary method of disposal in the United States (US) is unsustainable. In 2018 the US Environmental Protection Agency (EPA) estimated that the commercial, institutional, and residential sectors of the US economy wasted 63 million metric tons of food, most of which was sent to landfills. Instead, food waste could be recycled and used to supplement or replace commercial feed in aquaculture to sustainably stimulate production of fish. Zebrafish (Danio rerio) are a well characterized animal model closely related to other fish species that are important …

Evaluating The Effects Of Antibody-Conjugated Multi-Walled Carbon Nanotubes In Combination With Microwave Irradiation, Amy Chall

Electronic Theses and Dissertations

Cancer remains one of the largest public health concerns of our day, particularly in developed countries where technological advances have allowed populations to live well into their eighth decade. In America, those in their 80’s have a 1 in 2 chance of developing cancer in their lifetime. Prostate cancer, specifically is the second leading cause of cancer deaths in males. Traditional cancer therapies cause high levels of toxicity to the patient due to mechanisms of action that often attack cancer cells and healthy cells alike. The holy grail of cancer research is to find a treatment that targets the cancer …

The Role Of Integrin Alpha 6 On Tumor Metastasis, Shauntell N. Luke Ms.

Honors College Theses

Cancer research is a large topic in science, because over 14 million people are diagnosed worldwide, and of those, 8 million will die annually. Traditional therapies are the mainstays of treatment strategies, but a cure less invasive or with less side effects have not yet been identified. In order to develop a better cure, we need to understand the tumor and molecules present in its tissues. Integrins, cell surface proteins that aid in cell communication, have been found in the tumor tissues of several types of cancers, including prostate, lung and bone. Integrin alpha-6 (ITGA6), in particular, has been found …

The Behavioral, Biochemical And Genetic Effects Of Sleep Deprivation In Zebrafish (Danio Rerio), Jade Catherine Boykin

Electronic Theses and Dissertations

Vertebrate sleep is a universal phenomenon and encompasses an array of conserved behavioral and physiological characteristics. Studies of sleep in humans have shown that sleep deprivation leads to adverse effects to human health and additional stress. Teleosts have emerged as a useful model species to study the vertebrate stress response, given its stress axis has high levels of homology with the mammals, including humans. For this study, the behavior of zebrafish (Danio rerio) subjected to acute (24 hours) and chronic (20 days) extended light exposure was monitored and whole-body cortisol was analyzed as an indication of activation of …

Developmental Toxicity Of Tetrakis (Hydromethyl) Phosphonium Chloride (Thpc) In Zebrafish (Danio Rerio) Embryos, Lan L. Nguyen

Honors College Theses

Tetrakis (hydromethyl) phosphonium chloride (THPC) is a tetrakis (hydromethyl) phosphonium salt commonly used by the textile industry and is polymerized onto cotton fabrics to provide a flame-retardant finish. Traces of THPC were found in the Ogeechee River, a 294 mile-long black river in Georgia, and could have been a toxin contributing to the mass killing of aquatic animals in May 2011. THPC has been known to be carcinogenic and cause other systemic toxicity in various mammalian animals. However given that the chemical is mostly discharged in natural waters, the environmental impact of the discharge on water quality and there by …

Myosinx Is Required For Craniofacial Development In Danio Rerio, Cole Yancey

Electronic Theses and Dissertations

Craniofacial development is the process of laying early cartilage and bone patterns in the anterior region of the embryo, which ultimately results in shaping the structure of the face and head of an organism. Craniofacial abnormalities in humans, such as cleft lip and palate, are among the most common of all birth defects. Therefore, investigating the molecular mechanisms involved in craniofacial development will help us understand both evolutionary processes and genetic diseases. Craniofacial cartilage and bone structures are almost entirely derived from neural crest cells. Neural crest are a pluripotent migratory stream of cells that originate from the early developing …

Myosin 10 Is Required For Spinal Motor Axon Growth And Guidance In Zebrafish Embryos, Crystal Ivey

Electronic Theses and Dissertations

Neurodevelopmental disorders are disabilities caused by malfunctioning mechanisms within the developing nervous tissue. These abnormalities often result in conditions such as autism spectrum disorders, Attention Deficient Hyperactivity Disorder (ADHD), motor dysfunctions, learning disabilities and mental retardation. Recent surveys indicate that there will be a 12% increase of children in the United States alone who are affected by neurodevelopmental disorders. Thus, it is important to understand both the normal and abnormal mechanisms of neural development. Neural development involves specification of new neurons and formation of neural circuits that connect the nervous system to every organ of the developing embryo. Neural circuits …