Open Access. Powered by Scholars. Published by Universities.®
- Discipline
Articles 1 - 6 of 6
Full-Text Articles in Life Sciences
Quantitative Dissection Of Clone-Specific Growth Rates In Cultured Malaria Parasites, Heather B. Reilly Ayala, Hongjian Wang, John A. Steuter, Anastasia M. Marx, Michael T. Ferdig
Quantitative Dissection Of Clone-Specific Growth Rates In Cultured Malaria Parasites, Heather B. Reilly Ayala, Hongjian Wang, John A. Steuter, Anastasia M. Marx, Michael T. Ferdig
Faculty Publications - Department of Biological & Molecular Science
Measurement of parasite proliferation in cultured red blood cells underpins many facets of malaria research, from drug sensitivity assays to assessing the impact of experimentally altered genes on parasite growth, virulence, and fitness. Pioneering efforts to grow Plasmodium falciparum in cultured red blood cells revolutionized malaria research and spurred the development of semi-high throughput growth assays using radio-labeled hypoxanthine, an essential nucleic acid precursor, as a reporter of whole-cycle proliferation (Trager and Jensen, 1976; Desjardins et al., 1979). Use of hypoxanthine (Hx) and other surrogate readouts of whole-cycle proliferation remains the dominant choice in malaria research. While amenable to high-throughput …
Pyruvate Transport In Isolated Cardiac Mitochondria From Two Species Of Amphibian Exhibiting Dissimilar Aerobic Scope: Bufo Marinus And Rana Catesbeiana, Jeff Duerr, Kristina Tucker
Pyruvate Transport In Isolated Cardiac Mitochondria From Two Species Of Amphibian Exhibiting Dissimilar Aerobic Scope: Bufo Marinus And Rana Catesbeiana, Jeff Duerr, Kristina Tucker
Faculty Publications - Department of Biological & Molecular Science
Cardiac mitochondria were isolated from Bufo marinus and Rana catesbeiana, two species of amphibian whose cardiovascular systems are adapted to either predominantly aerobic or glycolytic modes of locomotion. Mitochondrial oxidative capacity was compared using VO2 max and respiratory control ratios in the presence of a variety of substrates including pyruvate, lactate, oxaloacetate, b-hydroxybutyrate, and octanoyl-carnitine. B. marinus cardiac mitochondria exhibited VO2 max values twice that of R. catesbeiana cardiac mitochondria when oxidizing carbohydrate substrates. Pyruvate transport was measured via a radiolabeled-tracer assay in isolated B. marinus and R. catesbeiana cardiac mitochondria. Time-course experiments described both a-cyano-4- hydroxycinnamate-sensitive (MCT-like) and phenylsuccinate-sensitive …
Expression And Localization Of An Aquaporin-1 Homologue In The Avian Kidney And Lower Intestinal Tract, G Casotti, T Waldron, G Misquith, Donald R. Powers, L Slusher
Expression And Localization Of An Aquaporin-1 Homologue In The Avian Kidney And Lower Intestinal Tract, G Casotti, T Waldron, G Misquith, Donald R. Powers, L Slusher
Faculty Publications - Department of Biological & Molecular Science
No abstract provided.
The Aerodynamics Of Hummingbird Flight, Douglas R. Warrick, Bret W. Tobalske, Donald R. Powers, Michael H. Dickenson
The Aerodynamics Of Hummingbird Flight, Douglas R. Warrick, Bret W. Tobalske, Donald R. Powers, Michael H. Dickenson
Faculty Publications - Department of Biological & Molecular Science
Hummingbirds fly with their wings almost fully extended during their entire wingbeat. This pattern, associated with having proportionally short humeral bones, long distal wing elements, and assumed to be an adaptation for extended hovering flight, has lead to predictions that the aerodynamic mechanisms exploited by hummingbirds during hovering should be similar to those observed in insects. To test these predictions, we flew rufous hummingbirds (Selasphorus rufus, 3.3 g, n = 6) in a variable–speed wind tunnel (0-12 ms-1) and measured wake structure and dynamics using digital particle image velocimetry (DPIV). Unlike hovering insects, hummingbirds produced 75% of their weight support …
Three-Dimensional Kinematics Of Hummingbird Flight, Bret W. Tobalske, Douglas R. Warrick, Christopher J. Clark, Donald R. Powers, Tyson L. Hendrick, Gabriel A. Hyder, Andrew A. Biewener
Three-Dimensional Kinematics Of Hummingbird Flight, Bret W. Tobalske, Douglas R. Warrick, Christopher J. Clark, Donald R. Powers, Tyson L. Hendrick, Gabriel A. Hyder, Andrew A. Biewener
Faculty Publications - Department of Biological & Molecular Science
Hummingbirds are specialized for hovering flight, and substantial research has explored this behavior. Forward flight is also important to hummingbirds, but the manner in which they perform forward flight is not well documented. Previous research suggests that hummingbirds increase flight velocity by simultaneously tilting their body angle and stroke-plane angle of the wings, without varying wingbeat frequency and upstroke: downstroke span ratio. We hypothesized that other wing kinematics besides stroke-plane angle would vary in hummingbirds. To test this, we used synchronized highspeed (500·Hz) video cameras and measured the threedimensional wing and body kinematics of rufous hummingbirds (Selasphorus rufus, 3·g, N=5) …
“Carbachol Regulation Of Erk And The Transcription Factor Elk-1 In Mcf-7 Breast Cancer Cells, John M. Schmitt, Andrea Wagner
“Carbachol Regulation Of Erk And The Transcription Factor Elk-1 In Mcf-7 Breast Cancer Cells, John M. Schmitt, Andrea Wagner
Faculty Publications - Department of Biological & Molecular Science
Abstract Cancer has numerous molecular, biochemical and physiological hallmarks including uncontrolled cell growth and proliferation. Previous studies on MCF-7 breast cancer cells have shown that both intracellular calcium levels and the extracellular signal-regulated protein kinase (ERK) is activated downstream of the G-protein coupled receptor (GPCR) agonist, carbachol. Calcium/calmodulin regulate the calcium/calmodulin-dependent kinase (CaM Ks) family of proteins that have been proposed to regulate ERK and transcription. Our goal was to determine the mechanism of carbachol activation on ERK and the transcription factor Elk-1 in MCF-7 cells. Our results suggest that 10 μM carbachol treatment of MCF-7 cells triggers ERK1/2 phosphorylation …