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Full-Text Articles in Life Sciences

Antimicrobial Producing Bacteria Isolated From Petroleum-Laced Hypersaline Soil, Joanna L. Fay May 2013

Antimicrobial Producing Bacteria Isolated From Petroleum-Laced Hypersaline Soil, Joanna L. Fay

Master's Theses

Given the alternative functions of “antibiotics” as communication molecules or participants in metabolism, it seems probable that production might be influenced by factors such as nutrient availability, interactions with neighboring microbes, and/or colony or community structure and maturity. With this in mind, the present study aimed to broaden the scope of the search for novel antibiotics by experimenting with the following parameters: source of bacterial isolation, growth and assay media, and culturing techniques. Bacteria for this study were isolated from two categories of soil (petroleum-contaminated or uncontaminated) to compare diversity and antimicrobial activity. Compared to the uncontaminated soil, isolates of …


Influence Of Wind Turbines On Mammalian Occupancy Patterns, Brian P. Tanis May 2013

Influence Of Wind Turbines On Mammalian Occupancy Patterns, Brian P. Tanis

Master's Theses

Wind energy is among the most rapidly growing energy industries in the United States, with support for development coming from both state and federal governments. While the industry depicts an environmentally friendly image, the addition of infrastructure associated with wind farms alters landscapes in novel ways. Numerous studies have documented impacts wind turbines have on bird and bat mortality; however, far less attention has been directed towards responses of non-volant, terrestrial organisms. Mammalian mesocarnivores are model organisms to assess the alteration of communities surrounding wind turbines as they respond to addition of turbines and human activity, addition and improvement of …


Effects Of Long-Term Exposure To Kanamycin And/Or Ampicillin On Resistance Genes On An E. Coli Plasmid, Justin W.R. Kerby Ii May 2013

Effects Of Long-Term Exposure To Kanamycin And/Or Ampicillin On Resistance Genes On An E. Coli Plasmid, Justin W.R. Kerby Ii

Master's Theses

Multi-drug-resistant bacteria are a major cause of hospital-acquired infections and antibiotic resistance in these organisms is often plasmid mediated, which has become a growing concern. Plasmids conferring resistance to multiple antibiotics are increasingly becoming a common source of antibiotic resistance. The behavior of these plasmids under and in the absence of selective pressure is not yet fully understood. Therefore, to determine the behavior of a multiple-resistance plasmid under selective pressure pCR 2.1-TOPO, a commercial plasmid, was inserted into an Escherichia coli host and grown in a continuous culture under four conditions: broth with 1) kanamycin alone, 2) ampicillin alone, 3) …


Stable Isotope Analysis Of Two Mephitidae Species Reflects Population Trends And Landscape Structure, Amanda E. Cheeseman May 2013

Stable Isotope Analysis Of Two Mephitidae Species Reflects Population Trends And Landscape Structure, Amanda E. Cheeseman

Master's Theses

The eastern spotted skunk (Spilogale putorius) is a small omnivorous Carnivora similar in much of its natural history to the commonly found striped skunk (Mephitis mephitis). Spilogale putorius has experienced drastic population declines over a large portion of its geographic range. Many hypotheses for the decline of S. putorius have been proposed. δ13C and δ15N isotope analysis provides a unique opportunity to examine diet over an expanded time span. Thus was used on hair sampled from natural history collections, teaching collections, and road kills to examine dietary change for both S. putorius and M. mephitis from 1852 to 2012. Because …