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Full-Text Articles in Life Sciences

Cloning And Characterization Of Farnesyl Diphosphate Synthase Gene Involved In Triterpenoids Biosynthesis From Poria Cocos, Jianrong Wang, Yangyuan Li, Danni Liu Dec 2014

Cloning And Characterization Of Farnesyl Diphosphate Synthase Gene Involved In Triterpenoids Biosynthesis From Poria Cocos, Jianrong Wang, Yangyuan Li, Danni Liu

Biomedical Engineering and Sciences Faculty Publications

Poria cocos (P. cocos) has long been used as traditional Chinese medicine and triterpenoids are the most important pharmacologically active constituents of this fungus. Farnesyl pyrophosphate synthase (FPS) is a key enzyme of triterpenoids biosynthesis. The gene encoding FPS was cloned from P. cocos by degenerate PCR, inverse PCR and cassette PCR. The open reading frame of the gene is 1086 bp in length, corresponding to a predicted polypeptide of 361 amino acid residues with a molecular weight of 41.2 kDa. Comparison of the P. cocos FPS deduced amino acid sequence with other species showed the highest identity with Ganoderma …


Metamorphosis Of Limulus Polyphemus Trilobite Larvae: Role Of Chemical And Structural Cues, Competency, And The Cost Of Delayed Metamorphosis, Stephanie Marie Kronstadt Dec 2014

Metamorphosis Of Limulus Polyphemus Trilobite Larvae: Role Of Chemical And Structural Cues, Competency, And The Cost Of Delayed Metamorphosis, Stephanie Marie Kronstadt

Theses and Dissertations

Many marine and estuarine arthropods exhibit complex life cycles that include planktonic larval stages and benthic juvenile and adult phases. Chemical and structural cues associated with juvenile habitats often induce settlement and metamorphosis, thereby shortening the duration of the larval phase. These cues can trigger metamorphosis only after larvae reach competency, or developmental maturity. The point at which larvae reach this competency period and the ability to retain competency is highly species specific. Once competency is attained, a decrease in the time to metamorphosis (TTM) can decrease dispersal potential but may increase the chance of settling in a suitable habitat. …


Effects Of Ocean Acidification On The Dissolution Rates Of Reef-Coral Skeletons, Robert Van Woesik, Kelly Van Woesik, Liana Van Woesik, Sandra Van Woesik Nov 2014

Effects Of Ocean Acidification On The Dissolution Rates Of Reef-Coral Skeletons, Robert Van Woesik, Kelly Van Woesik, Liana Van Woesik, Sandra Van Woesik

Ocean Engineering and Marine Sciences Faculty Publications

Ocean acidification threatens the foundation of tropical coral reefs. This study investigated three aspects of ocean acidification: (i) the rates at which perforate and imperforate coral-colony skeletons passively dissolve when pH is 7.8, which is predicted to occur globally by 2100, (ii) the rates of passive dissolution of corals with respect to coral-colony surface areas, and (iii) the comparative rates of a vertical reef-growth model, incorporating passive dissolution rates, and predicted sea-level rise. By 2100, when the ocean pH is expected to be 7.8, perforate Montipora coral skeletons will lose on average 15 kg CaCO3 m􀀀2 y􀀀1, which is approximately …


Genetic Susceptibility, Colony Size, And Water Temperature Drive White-Pox Disease On The Coral Acropora Palmata, Erinn M. Muller, Robert Van Woesik Nov 2014

Genetic Susceptibility, Colony Size, And Water Temperature Drive White-Pox Disease On The Coral Acropora Palmata, Erinn M. Muller, Robert Van Woesik

Ocean Engineering and Marine Sciences Faculty Publications

Outbreaks of coral diseases are one of the greatest threats to reef corals in the Caribbean, yet the mechanisms that lead to coral diseases are still largely unknown. Here we examined the spatial-temporal dynamics of white-pox disease on Acropora palmata coral colonies of known genotypes. We took a Bayesian approach, using Integrated Nested Laplace Approximation algorithms, to examine which covariates influenced the presence of white-pox disease over seven years. We showed that colony size, genetic susceptibility of the coral host, and high-water temperatures were the primary tested variables that were positively associated with the presence of white-pox disease on A. …


Pre-Stimulus Neural Activity Predicts Successful Encoding Of Inter-Item Associations, Richard J. Addante, Marianne De Chastelaine, Michael D. Rugg Oct 2014

Pre-Stimulus Neural Activity Predicts Successful Encoding Of Inter-Item Associations, Richard J. Addante, Marianne De Chastelaine, Michael D. Rugg

Psychology Faculty Publications

fMRI was employed to investigate the relationship between pre-stimulus neural activity and associative encoding of words and pictures in humans. While undergoing scanning, subjects studied randomly interleaved word or picture pairs. A pre-stimulus cue preceded the presentation of each study pair and signaled whether it would comprise words or pictures. Memory for the study pairs was later tested with an associative recognition test, which comprised word or picture pairs presented either in the same (intact) or a different (rearranged) pairing as at study, along with pairs of new items. The critical fMRI contrast was between study activity associated with pairs …


Persistence And Change In Community Composition Of Reef Corals Through Present, Past, And Future Climates, Robert Van Woesik, Peter J. Edmunds, Mehdi Adjeroud, Marissa L. Baskett, Iliana B. Baums, Ann F. Budd, Robert C.C. Carpenter, Nicholas S. Fabina, Tungyung Fan, Eric C. Franklin Oct 2014

Persistence And Change In Community Composition Of Reef Corals Through Present, Past, And Future Climates, Robert Van Woesik, Peter J. Edmunds, Mehdi Adjeroud, Marissa L. Baskett, Iliana B. Baums, Ann F. Budd, Robert C.C. Carpenter, Nicholas S. Fabina, Tungyung Fan, Eric C. Franklin

Ocean Engineering and Marine Sciences Faculty Publications

The reduction in coral cover on many contemporary tropical reefs suggests a different set of coral community assemblages will dominate future reefs. To evaluate the capacity of reef corals to persist over various time scales, we examined coral community dynamics in contemporary, fossil, and simulated future coral reef ecosystems. Based on studies between 1987 and 2012 at two locations in the Caribbean, and between 1981 and 2013 at five locations in the Indo-Pacific, we show that many coral genera declined in abundance, some showed no change in abundance, and a few coral genera increased in abundance. Whether the abundance of …


Genetic Variations In Two Seahorse Species (Hippocampus Mohnikei And Hippocampus Trimaculatus): Evidence For Middle Pleistocene Population Expansion, Yanhong Zhang, Nancy Kim Pham, Huixian Zhang, Junda Lin, Qiang Lin Aug 2014

Genetic Variations In Two Seahorse Species (Hippocampus Mohnikei And Hippocampus Trimaculatus): Evidence For Middle Pleistocene Population Expansion, Yanhong Zhang, Nancy Kim Pham, Huixian Zhang, Junda Lin, Qiang Lin

Ocean Engineering and Marine Sciences Faculty Publications

Population genetic of seahorses is confidently influenced by their species-specific ecological requirements and life-history traits. In the present study, partial sequences of mitochondrial cytochrome b (cytb) and control region (CR) were obtained from 50 Hippocampus mohnikei and 92 H. trimaculatus from four zoogeographical zones. A total of 780 base pairs of cytb gene were sequenced to characterize mitochondrial DNA (mtDNA) diversity. The mtDNA marker revealed high haplotype diversity, low nucleotide diversity, and a lack of population structure across both populations of H. mohnikei and H. trimaculatus. A neighbour-joining (NJ) tree of cytb gene sequences showed that H. mohnikei haplotypes formed …


Human Influences On Anuran Distribution Changes In The Southeastern U.S.A., Michelle Diane Luce Jul 2014

Human Influences On Anuran Distribution Changes In The Southeastern U.S.A., Michelle Diane Luce

Theses and Dissertations

As human populations continue to expand and grow, ecosystems and the species that inhabit them face ever-increasing stress. To conserve these ecosystems, scientists use indicator species to try to measure the impact of human-induced pressures on the environment. Anurans are exceptional indicator species because they are often the first vertebrates to respond to the direct and indirect effects of land use by humans. Sparse historical data have made it difficult to map the ‘natural’ distribution of many species and, therefore, to map any changes in distribution range as a result of human expansion. This study sought to address the lack …