Life Sciences Commons^™

Analysis Of Plant Analytes Using Capillary Electrophoresis And High Performance Liquid Chromatography, Umme Kalsoom

Theses: Doctorates and Masters

Plants contain an enormous array of organic and inorganic components, the analysis for which may involve a wide range of methods. The focus of this study was to develop high performance liquid chromatography and capillary electrophoresis methods for the analysis of three classes of analytes: osmoregulants, minerals and amino acids.

Firstly, this study explored the potential of capillary electrophoresis for the analysis of three very common osmoregulants (proline, glycine betaine and mannitol). A diverse array of methods has been reported for determining each of these analytes, however, the literature on osmoregulants and their analysis is quite disjointed and traverses both …

Development And Optimisation Of A Micellar Electrokinetic Capillary Chromatography Method For The Separation And Identification Of Phenolics From Eucalypt Species, Andrew J. Tromans

Theses : Honours

Several buffers were examined for their ability to separate a complex mixture of phenolic compounds using micellar electrokinetic capillary chromatography (MEKC). The phenolic mixture included simple phenols, phenolic acids and coumarins. Of the different buffers examined, 6 mM borate, 10 mM phosphate, 100 mM SDS, pH 8.3 gave the best separation. The ability of organic modifiers, complexation agents such as α cyclodextrin, β cyclodextrin and mixed SDS I Brij 35 micelles to improve the separation and peak retention time reproducibility of the buffer system was investigated. Baseline separation of the 18 phenolics was achieved by the 6 mM borate, 10 …