Open Access. Powered by Scholars. Published by Universities.®

Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

Edith Cowan University

Research outputs 2022 to 2026

Series

Environment

Articles 1 - 3 of 3

Full-Text Articles in Life Sciences

Environmental Contamination With Clostridioides (Clostridium) Difficile In Vietnam, Peng A. Khun, Long D. Phi, Huong T. T. Bui, Nguyen T. Bui, Quyen T. H. Vu, Luong D. Trinh, Deirdre A. Collins, Thomas V. Riley Jun 2023

Environmental Contamination With Clostridioides (Clostridium) Difficile In Vietnam, Peng A. Khun, Long D. Phi, Huong T. T. Bui, Nguyen T. Bui, Quyen T. H. Vu, Luong D. Trinh, Deirdre A. Collins, Thomas V. Riley

Research outputs 2022 to 2026

AIMS: To investigate the prevalence, molecular type, and antimicrobial susceptibility of Clostridioides difficile in the environment in Vietnam, where little is known about C. difficile. METHODS AND RESULTS: Samples of pig faeces, soils from pig farms, potatoes, and the hospital environment were cultured for C. difficile. Isolates were identified and typed by polymerase chain reaction (PCR) ribotyping. The overall prevalence of C. difficile contamination was 24.5% (68/278). Clostridioides difficile was detected mainly in soils from pig farms and hospital soils, with 70%-100% prevalence. Clostridioides difficile was isolated from 3.4% of pig faecal samples and 5% of potato surfaces. The four …


Complete Genome Sequences Of Evolutionary Clade C-Iii Strains Of Clostridioides (Clostridium) Difficile Isolated From The Environment In Western Australia, Nirajmohan Shivaperumal, Natasza M. R. Hain-Saunders, Barbara J. Chang, Thomas V. Riley, Daniel R. Knight May 2023

Complete Genome Sequences Of Evolutionary Clade C-Iii Strains Of Clostridioides (Clostridium) Difficile Isolated From The Environment In Western Australia, Nirajmohan Shivaperumal, Natasza M. R. Hain-Saunders, Barbara J. Chang, Thomas V. Riley, Daniel R. Knight

Research outputs 2022 to 2026

Clostridioides (Clostridium) difficile in the environment is thought to contribute to C. difficile infection in community settings. Here, we provide complete genome assemblies for two esculin hydrolysis-negative strains of C. difficile that were isolated from soils in Western Australia; the strains produce white colonies on chromogenic media and belong to evolutionarily divergent clade C-III.


Esculin Hydrolysis Negative And Tcda-Only Producing Strains Of Clostridium (Clostridioides) Difficile From The Environment In Western Australia, Nirajmohan Shivaperumal, Daniel R. Knight, Korakrit Imwattana, Grace O. Androga, Barbara J. Chang, Thomas V. Riley Sep 2022

Esculin Hydrolysis Negative And Tcda-Only Producing Strains Of Clostridium (Clostridioides) Difficile From The Environment In Western Australia, Nirajmohan Shivaperumal, Daniel R. Knight, Korakrit Imwattana, Grace O. Androga, Barbara J. Chang, Thomas V. Riley

Research outputs 2022 to 2026

Background and Aims: Clostridium (Clostridiodes) difficile clade 3 ribotype (RT) 023 strains that fail to produce black colonies on bioMérieux ChromID agar have been reported, as well as variant strains of C. difficile that produce only toxin A. We have recently isolated strains of C. difficile from the environment in Western Australia (WA) with similar characteristics. The objective of this study was to characterize these strains. It was hypothesized that a putative β-glucosidase gene was lacking in these strains of C. difficile, including RT 023, leading to white colonies. Methods and Results: A total of 17 environmental isolates of C. …