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Structural And Functional Analysis Of Grapefruit Flavonol-Specific-3-O-Gt Mutant P145t, Sangam Kandel Mr
Structural And Functional Analysis Of Grapefruit Flavonol-Specific-3-O-Gt Mutant P145t, Sangam Kandel Mr
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This research is focused on the study of the effect of mutating proline 145 to threonine on the substrate and regiospecificity of flavonol specific 3-O-glucosyltransferase (Cp3GT). While the mutant P145T enzyme did not glucosylate anthocyanidins, it did glucosylate flavanones and flavones in addition to retaining activity with flavonols. HPLC was used for product identification and showed mutant P145T glucosylated naringenin at the 7-OH position forming naringenin-7-O-glucoside and flavonols at the 3-OH position. Homology modeling and docking was done to predict the acceptor substrate recognition pattern and models were validated by experimental results. In other related work, …