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Articles 1 - 13 of 13
Full-Text Articles in Life Sciences
Breeding Bird Census To Compare Long-Term Changes In The Avifauna Of The Spruce-Fir Forest On Mount Guyot, Great Smoky Mountains National Park 1967-2015, Kevin C. Brooks
Breeding Bird Census To Compare Long-Term Changes In The Avifauna Of The Spruce-Fir Forest On Mount Guyot, Great Smoky Mountains National Park 1967-2015, Kevin C. Brooks
Electronic Theses and Dissertations
The high-elevation forests of the Southern Appalachian Mountains have been impacted and rearranged by a tiny introduced pest from Europe, known as the Balsam Woolly Adelgid (Adelges piceaea), creating a concern for conservation. Breeding bird censuses, along with botanical surveys, have been conducted periodically on an established 60-acre plot since 1967 on the virgin forested slopes of Mount Guyot, Great Smoky Mountains National Park, with the last census being completed in 2015. Breeding bird populations are shown to rise and fall in response to the forest’s changes over the last 48 years. Comparisons are made between all studies in order …
Influence Of Lactobacillus Rhamnosus Isolated From “Amabere Amaruranu” Cultured Milk On Adipogenesis, Justin E. Kotala
Influence Of Lactobacillus Rhamnosus Isolated From “Amabere Amaruranu” Cultured Milk On Adipogenesis, Justin E. Kotala
Electronic Theses and Dissertations
This study was performed to test the in vitro effects of a Lactobacillus rhamnosus isolate from “amabere amaruranu”, a traditional Kenyan cultured milk, on 3T3-L1 and Caco-2 cell lines. Cultures of fully mature 3T3-L1 adipocytes were treated with bacterial isolate cell extract (CE), filtered spent broth (FSB) from overnight bacterial culture, or with a PBS control. Expression levels of PPAR³1 and 2, C/EBP±, and ATGL proteins in 3T3-L1 cells were upregulated by FSB treatment. CE treatment did not affect protein expression levels. Expression of MTTP and SREBP-1c proteins in Caco-2 cells showed no change with either treatment. Optical density measurements …
Effects Of Complex Formation Of Dna With Positively Charged Polyamines And Polypeptides On The Products Of Oxidative Damage To Dna 2-Deoxyribose By Hydroxyl Radicals, Modeste N. Tegomoh
Effects Of Complex Formation Of Dna With Positively Charged Polyamines And Polypeptides On The Products Of Oxidative Damage To Dna 2-Deoxyribose By Hydroxyl Radicals, Modeste N. Tegomoh
Electronic Theses and Dissertations
It is known that histones and other DNA-binding polycations protect DNA from radiation damage mediated by hydroxyl radicals. Until recently, this protection of DNA has mainly been attributed to compaction and aggregation. It was hypothesized that chemical repair of DNA sugar radicals by donation of hydrogen atom from polycations also significantly contributes to DNA protection. To test this hypothesis, the relative yields of low-molecular weight characteristic products of oxidation of DNA sugar were compared in X-irradiated samples of naked DNA and DNA complexes with a number of polycations by using an HPLC-based method of DNA damage product quantification. The variation …
Characterization Of Sbip68: A Putative Tobacco Glucosyltransferase Protein And Its Role In Plant Defense Mechanisms, Abdulkareem O. Odesina
Characterization Of Sbip68: A Putative Tobacco Glucosyltransferase Protein And Its Role In Plant Defense Mechanisms, Abdulkareem O. Odesina
Electronic Theses and Dissertations
Plant secondary metabolites are essential for normal growth and development in plants ultimately affecting crop yield. They play roles ranging from appearance of the plants to defending against pathogen attack and herbivory. They have been used by humans for medicinal and recreational purposes amongst others. Glycosyltransferases catalyze the transfer of sugars from donor substrates to acceptors. Glucosyltransferases are a specific type of glycosyltransferases known to transfer glucose molecules from a glucose donor to a glucose acceptor (aglycone) producing the corresponding glucose secondary metabolite or glycone, in this case glucosides. It was hypothesized that SBIP68, a tobacco putative glucosyltransferase-like protein glucosylated …
Algr Directly Controls Rsma In Pseudomonas Aeruginosa, Tyler Speaks
Algr Directly Controls Rsma In Pseudomonas Aeruginosa, Tyler Speaks
Electronic Theses and Dissertations
Pseudomonas aeruginosa is a bacterial pathogen that can infect any human tissue. The lungs of cystic fibrosis patients become chronically infected with Pseudomonas aeruginosa. Virulence factor gene expression is under elaborate regulatory control that remains poorly characterized. Understanding the regulatory hierarchy involved during infection is essential for identifying novel drug targets. RsmA is a post-transcriptional regulatory protein that controls expression of several virulence factors. Previous studies demonstrated alginate regulatory components AlgU and AlgR as regulators of rsmA expression. The aim of this study was to determine how AlgR controls rsmA expression. Western blot analysis of HA-tagged RsmA confirmed lower …
Identification Of Genes Required To Synthesize An Antibiotic-Like Compound From The Soil Bacterium Rhodococcus Sp. Mtm3w5.2, Amber L. Ward
Identification Of Genes Required To Synthesize An Antibiotic-Like Compound From The Soil Bacterium Rhodococcus Sp. Mtm3w5.2, Amber L. Ward
Electronic Theses and Dissertations
Rhodococcus is a soil bacterium, member of the Actinobacteria, and a close relative of the prolific small molecule producer Streptomyces. Recent interest in Rhodococcus as an under investigated source of possible bioactive secondary metabolites is sparked by the discovery of many polyketide synthase and non-ribosomal peptide synthetase genes of unknown function from sequenced Rhodococcus genomes. Rhodococcus species strain MTM3W5.2 was recently shown to produce a strong inhibitory compound with activity against most strains of Rhodococcus and closely related genera. A goal of this investigation is to discover the gene(s) required to synthesize this inhibitory molecule. The engineered Rhodococcus …
An Investigation Of The Relationship Between A Static Jump Protocol And Squat Strength: A Potential Protocol For Collegiate Strength And Explosive Athlete Monitoring, Cody Haun
Electronic Theses and Dissertations
The purpose of this study was to examine the relationship between estimated absolute and relative squat strength and a static jump protocol with potential to provide desirable training adaptation data to practitioners in the field of collegiate strength and conditioning. Forty-one young (20.80 ± 2.44 years), healthy volunteers reported estimated back squat 1RM’s based on the most recent training block and completed a static jump protocol. Males (n=19, est. 1RM 141.29 ± 32.02kg) and female (n=22, est. 1RM 71.56 ± 19.64kg) jump data revealed large to very large correlations between squat strength, mean jump heights of jumps and other calculated …
The Effect Of Various Body Positions On Performance Of The Isometric Mid-Thigh Pull, George K. Beckham
The Effect Of Various Body Positions On Performance Of The Isometric Mid-Thigh Pull, George K. Beckham
Electronic Theses and Dissertations
The purpose of this dissertation was to evaluate the effects of changing body position on the execution of the isometric mid-thigh pull (IMTP). Furthermore, while there is evidence to suggest that there is an effect of familiarization on performance of maximal strength tests, there has been no known research evaluating the effect of learning on the IMTP. The effect of familiarization was assessed by evaluating changes in variables obtained from the IMTP. Subjects did not statistically improve over the five IMTP testing sessions, regardless of the body position used, or if subjects had previous experience with weightlifting derivatives. This may …
An Analysis Of The Interaction Of Methylphenidate And Nicotine In Adolescent Rats: Effects On Bdnf, Elizabeth D. Freeman
An Analysis Of The Interaction Of Methylphenidate And Nicotine In Adolescent Rats: Effects On Bdnf, Elizabeth D. Freeman
Electronic Theses and Dissertations
This investigation was an analysis of the interaction of adolescent exposure to methylphenidate (MPH; trade name: Ritalin) on nicotine sensitization and conditioned place preference (CPP) in a rodent model and underlying mechanisms of this effect. Animals were treated IP with 1 mg/kg MPH or saline using a ―school day‖ regimen of five days on, two days off, from postnatal day (P) 28-50. During the final two weeks of MPH treatment, animals were either behaviorally sensitized to nicotine (0.5 mg/kg free base) or saline for 10 days, or conditioned to nicotine or saline using the CPP behavioral paradigm. In addition, three …
Move, Interact, And Connect Personally Barter Theatre’S Project Real Gets Implicit In Order To Learn, Megan E. Atkinson
Move, Interact, And Connect Personally Barter Theatre’S Project Real Gets Implicit In Order To Learn, Megan E. Atkinson
Electronic Theses and Dissertations
Body movement, hands-on activity, embodiment, social interaction, emotions, and self-reflection allow teaching artists of Barter’s Theatre’s Project REAL to conduct a lesson with an implicit learning experience as the focus. Barter Theatre’s Project REAL exists as a theatre for education program that collaborates with regular classroom teachers on delivering the curriculum through specific theatre exercises in order to connect the material personally to the students’ lives. Theatre tools provide a human experience that enhances learning for the student by use of kinesthetic movement, social learning, emotions and interpersonal skills. To understand the effects of Barter Theatre’s Project REAL, the director …
Characterization Of Putative Exbb And Exbd Leads To The Identification Of A Potential Tol-Pal System In Rhizobium Leguminosarum Atcc 14479, Valeria Barisic
Characterization Of Putative Exbb And Exbd Leads To The Identification Of A Potential Tol-Pal System In Rhizobium Leguminosarum Atcc 14479, Valeria Barisic
Electronic Theses and Dissertations
Rhizobium leguminosarum is a Gram negative nitrogen-fixing soil bacterium. Due to the limited bioavailability of iron, bacteria utilize siderophores that scavenge and bind available iron. The transport of iron-siderophore complexes is achieved by the TonB-ExbB-ExbD complex. We have previously shown that a functional TonB protein is necessary for iron transport by creating ΔtonB mutants and assessing their growth and 55Fe-siderophore transport ability. We attempted to identify and characterize the roles of putative exbB and exbD genes using a similar approach. Growth curves and sequence analyses suggest putative exbB and exbD may be the tolpal-associated genes tolQ and tolR. Phenotypic and …
A Hierarchical Graph For Nucleotide Binding Domain 2, Samuel Kakraba
A Hierarchical Graph For Nucleotide Binding Domain 2, Samuel Kakraba
Electronic Theses and Dissertations
One of the most prevalent inherited diseases is cystic fibrosis. This disease is caused by a mutation in a membrane protein, the cystic fibrosis transmembrane conductance regulator (CFTR). CFTR is known to function as a chloride channel that regulates the viscosity of mucus that lines the ducts of a number of organs. Generally, most of the prevalent mutations of CFTR are located in one of two nucleotide binding domains, namely, the nucleotide binding domain 1 (NBD1). However, some mutations in nucleotide binding domain 2 (NBD2) can equally cause cystic fibrosis. In this work, a hierarchical graph is built for NBD2. …
A Systematic Review Of The Soricimorph Eulipotyphla (Soricidae: Mammalia) From The Gray Fossil Site (Hemphillian), Tennessee, Joshua Doby
Electronic Theses and Dissertations
Due in part to the incompleteness of the Cenozoic fossil record in the eastern U.S., the evolution and immigration of shrews (Soricidae) is not well understood. A rich soricid fauna from the Gray Fossil Site (GFS), Washington County, TN, has enabled many new inferences to be made. There are 7 new species in 6 genera: Paenelimnoecus, “Blarinella”, Petenyia, Tregosorex, Crusafontina, and Gen et sp. nov. GFS species of the genera Paenelimnoecus, “Blarinella”, and Petenyia are the first occurrence of each genus in the New World. Tregosorex, Crusafontina, and the N.A. taxon Limnoecus all have their latest documented occurrence at the …